Effects of sgRNA length and number on gene editing efficiency and predicted mutations generated in rice
CRISPR-Cas9 is a common tool for gene editing, and appropriate sgRNAs are the key factor for successful editing. In this study, the effect of sgRNA length and number on editing efficiency was analyzed in rice using CYP81A6 as the target gene. A series of CRISPR-Cas9 plant expression vectors containi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2022-04-01
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| Series: | Crop Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514121001537 |