Biodegradation of PBSA Films by Elite <i>Aspergillus</i> Isolates and Farmland Soil
Plastic films are widely used in current agricultural practices; however, most mulch films used are discarded and buried in the land after harvest, having adverse environmental impacts. To solve this environmental problem, the demand for biodegradable mulch has been increasing in recent years. Polyb...
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MDPI AG
2022-03-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/7/1320 |
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| author | Hsiao-Lin Chien Yi-Ting Tsai Wei-Sung Tseng Jin-An Wu Shin-Liang Kuo Sheng-Lung Chang Shu-Jiuan Huang Chi-Te Liu |
| author_facet | Hsiao-Lin Chien Yi-Ting Tsai Wei-Sung Tseng Jin-An Wu Shin-Liang Kuo Sheng-Lung Chang Shu-Jiuan Huang Chi-Te Liu |
| author_sort | Hsiao-Lin Chien |
| collection | DOAJ |
| description | Plastic films are widely used in current agricultural practices; however, most mulch films used are discarded and buried in the land after harvest, having adverse environmental impacts. To solve this environmental problem, the demand for biodegradable mulch has been increasing in recent years. Polybutylene succinate-co-adipate (PBSA) is a biodegradable polymer with good ductility and can be used for packaging and mulching. In this study, we isolated two elite fungal strains for PBSA degradation from farmlands, i.e., <i>Aspergillus fumigatus</i> L30 and <i>Aspergillus terreus</i> HC, and the latter showed better degradation ability than the former. It is noteworthy that biodegradation of PBSA by <i>A. terreus</i> is reported for the first time, which revealed unique characteristics. In the soil burial test, even the soil with relatively poor degradation ability could be improved by the addition of elite fungal mycelia. In substrate specificity analyses of soil samples, PBSA could induce the synthesis of lipolytic enzymes of indigenous microbes to degrade substrates with medium and long carbon chains in soil. Furthermore, PBSA residues or fungal mycelia supplementation in soils had no adverse effect on the seed germination rate, seedling growth, or mature plant weight of the test green leafy vegetable. Taken together, the results of this study not only advance our understanding of the biodegradation of PBSA films by filamentous fungi but also provide insight into improving the efficiency of biodegradation in soil environments. |
| first_indexed | 2024-03-09T11:30:33Z |
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| id | doaj.art-650eb6d398904b90911418421603f3e2 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T11:30:33Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-650eb6d398904b90911418421603f3e22023-11-30T23:52:50ZengMDPI AGPolymers2073-43602022-03-01147132010.3390/polym14071320Biodegradation of PBSA Films by Elite <i>Aspergillus</i> Isolates and Farmland SoilHsiao-Lin Chien0Yi-Ting Tsai1Wei-Sung Tseng2Jin-An Wu3Shin-Liang Kuo4Sheng-Lung Chang5Shu-Jiuan Huang6Chi-Te Liu7Institute of Biotechnology, National Taiwan University, Taipei 106, TaiwanInstitute of Biotechnology, National Taiwan University, Taipei 106, TaiwanInstitute of Biotechnology, National Taiwan University, Taipei 106, TaiwanMaterial and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu 300, TaiwanMaterial and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu 300, TaiwanMaterial and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu 300, TaiwanMaterial and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu 300, TaiwanInstitute of Biotechnology, National Taiwan University, Taipei 106, TaiwanPlastic films are widely used in current agricultural practices; however, most mulch films used are discarded and buried in the land after harvest, having adverse environmental impacts. To solve this environmental problem, the demand for biodegradable mulch has been increasing in recent years. Polybutylene succinate-co-adipate (PBSA) is a biodegradable polymer with good ductility and can be used for packaging and mulching. In this study, we isolated two elite fungal strains for PBSA degradation from farmlands, i.e., <i>Aspergillus fumigatus</i> L30 and <i>Aspergillus terreus</i> HC, and the latter showed better degradation ability than the former. It is noteworthy that biodegradation of PBSA by <i>A. terreus</i> is reported for the first time, which revealed unique characteristics. In the soil burial test, even the soil with relatively poor degradation ability could be improved by the addition of elite fungal mycelia. In substrate specificity analyses of soil samples, PBSA could induce the synthesis of lipolytic enzymes of indigenous microbes to degrade substrates with medium and long carbon chains in soil. Furthermore, PBSA residues or fungal mycelia supplementation in soils had no adverse effect on the seed germination rate, seedling growth, or mature plant weight of the test green leafy vegetable. Taken together, the results of this study not only advance our understanding of the biodegradation of PBSA films by filamentous fungi but also provide insight into improving the efficiency of biodegradation in soil environments.https://www.mdpi.com/2073-4360/14/7/1320polybutylene succinate-co-adipate (PBSA)biodegradation<i>Aspergillus</i>phytotoxicityecotoxicitylipolytic enzyme |
| spellingShingle | Hsiao-Lin Chien Yi-Ting Tsai Wei-Sung Tseng Jin-An Wu Shin-Liang Kuo Sheng-Lung Chang Shu-Jiuan Huang Chi-Te Liu Biodegradation of PBSA Films by Elite <i>Aspergillus</i> Isolates and Farmland Soil Polymers polybutylene succinate-co-adipate (PBSA) biodegradation <i>Aspergillus</i> phytotoxicity ecotoxicity lipolytic enzyme |
| title | Biodegradation of PBSA Films by Elite <i>Aspergillus</i> Isolates and Farmland Soil |
| title_full | Biodegradation of PBSA Films by Elite <i>Aspergillus</i> Isolates and Farmland Soil |
| title_fullStr | Biodegradation of PBSA Films by Elite <i>Aspergillus</i> Isolates and Farmland Soil |
| title_full_unstemmed | Biodegradation of PBSA Films by Elite <i>Aspergillus</i> Isolates and Farmland Soil |
| title_short | Biodegradation of PBSA Films by Elite <i>Aspergillus</i> Isolates and Farmland Soil |
| title_sort | biodegradation of pbsa films by elite i aspergillus i isolates and farmland soil |
| topic | polybutylene succinate-co-adipate (PBSA) biodegradation <i>Aspergillus</i> phytotoxicity ecotoxicity lipolytic enzyme |
| url | https://www.mdpi.com/2073-4360/14/7/1320 |
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