Dynamics of Transgenic Enterobacter cloacae Expressing Green Fluorescent Protein-Defensin (GFP-D) in Anopheles stephensi under Laboratory Condition
Background: Enterobacter cloacae bacterium is a known symbiont of the most Anopheles gut microflora and nominated as a good candidate for paratransgenic control of malaria. However, the population dynamics of this bacterium within An. stephensi and its introduction methods to the mosquitoes have no...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2017-12-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/778 |
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| author | Hossein Dehghan Mohammad Ali Oshaghi Seyed Hassan Moosa-Kazemi Bagher Yakhchali Hassan Vatandoost Naseh Maleki-Ravasan Yavar Rassi Habib Mohammadzadeh Mohammad Reza Abai Fatemeh Mohtarami |
| author_facet | Hossein Dehghan Mohammad Ali Oshaghi Seyed Hassan Moosa-Kazemi Bagher Yakhchali Hassan Vatandoost Naseh Maleki-Ravasan Yavar Rassi Habib Mohammadzadeh Mohammad Reza Abai Fatemeh Mohtarami |
| author_sort | Hossein Dehghan |
| collection | DOAJ |
| description | Background: Enterobacter cloacae bacterium is a known symbiont of the most Anopheles gut microflora and nominated as a good candidate for paratransgenic control of malaria. However, the population dynamics of this bacterium within An. stephensi and its introduction methods to the mosquitoes have not yet been explored.
Methods: Enterobacter cloacae subsp. dissolvens expressing green fluorescent protein and defensin (GFP-D) was used to study transstadial transmission and the course of time, larval habitat, sugar, and blood meal on dynamics of the bacterium in the mosquito life stages in the laboratory condition. The bacterial quantities were measured by plating samples and counting GFP expressing colonies on the Tet-BHI agar medium.
Results: The E. cloacae population remained stable in sugar bait at least for eleven days whereas it was lowered in the insectary larval habitat where the bacteria inadequately recycled. The bacterium was weakly transmitted transstadially from larval to adult stage. The bacterial populations increased smoothly and then dramatically in the guts of An. stephensi following sugar and blood meal respectively followed by a gradual reduction over the time.
Conclusion: Enterobacter cloacae was highly stable in sugar bait and increased tremendously in the gut of female adult An. stephensi within 24h post blood meal. Sugar bait stations can be used for introduction of the transgenic bacteria in a paratransgenic approach. It is recommended to evaluate the attraction of sugar bait in combination with attractive kairomones as well as its stability and survival rate in the semi-field or field conditions. |
| first_indexed | 2024-12-10T18:55:18Z |
| format | Article |
| id | doaj.art-650fe001e23948078aebeb5ca4e82874 |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-10T18:55:18Z |
| publishDate | 2017-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-650fe001e23948078aebeb5ca4e828742022-12-22T01:37:11ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712017-12-01114367Dynamics of Transgenic Enterobacter cloacae Expressing Green Fluorescent Protein-Defensin (GFP-D) in Anopheles stephensi under Laboratory ConditionHossein Dehghan0Mohammad Ali Oshaghi1Seyed Hassan Moosa-Kazemi2Bagher Yakhchali3Hassan Vatandoost4Naseh Maleki-Ravasan5Yavar Rassi6Habib Mohammadzadeh7Mohammad Reza Abai8Fatemeh Mohtarami9Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, IranDepartment Industrial and of Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Iran Department of Chemical Pollutants and Pesticides, Institute for Environmental Research, Tehran University of Medical Sciences, IranMalaria and Vector Research Group, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, IranDepartment of Parasitology and Mycology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Iran Department of Chemical Pollutants and Pesticides, Institute for Environmental Research, Tehran University of Medical Sciences, IranDepartment of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, IranBackground: Enterobacter cloacae bacterium is a known symbiont of the most Anopheles gut microflora and nominated as a good candidate for paratransgenic control of malaria. However, the population dynamics of this bacterium within An. stephensi and its introduction methods to the mosquitoes have not yet been explored. Methods: Enterobacter cloacae subsp. dissolvens expressing green fluorescent protein and defensin (GFP-D) was used to study transstadial transmission and the course of time, larval habitat, sugar, and blood meal on dynamics of the bacterium in the mosquito life stages in the laboratory condition. The bacterial quantities were measured by plating samples and counting GFP expressing colonies on the Tet-BHI agar medium. Results: The E. cloacae population remained stable in sugar bait at least for eleven days whereas it was lowered in the insectary larval habitat where the bacteria inadequately recycled. The bacterium was weakly transmitted transstadially from larval to adult stage. The bacterial populations increased smoothly and then dramatically in the guts of An. stephensi following sugar and blood meal respectively followed by a gradual reduction over the time. Conclusion: Enterobacter cloacae was highly stable in sugar bait and increased tremendously in the gut of female adult An. stephensi within 24h post blood meal. Sugar bait stations can be used for introduction of the transgenic bacteria in a paratransgenic approach. It is recommended to evaluate the attraction of sugar bait in combination with attractive kairomones as well as its stability and survival rate in the semi-field or field conditions.https://jad.tums.ac.ir/index.php/jad/article/view/778Bacterial dynamicEnterobacter cloacaeAnopheles stephensiParatransgenesis |
| spellingShingle | Hossein Dehghan Mohammad Ali Oshaghi Seyed Hassan Moosa-Kazemi Bagher Yakhchali Hassan Vatandoost Naseh Maleki-Ravasan Yavar Rassi Habib Mohammadzadeh Mohammad Reza Abai Fatemeh Mohtarami Dynamics of Transgenic Enterobacter cloacae Expressing Green Fluorescent Protein-Defensin (GFP-D) in Anopheles stephensi under Laboratory Condition Journal of Arthropod-Borne Diseases Bacterial dynamic Enterobacter cloacae Anopheles stephensi Paratransgenesis |
| title | Dynamics of Transgenic Enterobacter cloacae Expressing Green Fluorescent Protein-Defensin (GFP-D) in Anopheles stephensi under Laboratory Condition |
| title_full | Dynamics of Transgenic Enterobacter cloacae Expressing Green Fluorescent Protein-Defensin (GFP-D) in Anopheles stephensi under Laboratory Condition |
| title_fullStr | Dynamics of Transgenic Enterobacter cloacae Expressing Green Fluorescent Protein-Defensin (GFP-D) in Anopheles stephensi under Laboratory Condition |
| title_full_unstemmed | Dynamics of Transgenic Enterobacter cloacae Expressing Green Fluorescent Protein-Defensin (GFP-D) in Anopheles stephensi under Laboratory Condition |
| title_short | Dynamics of Transgenic Enterobacter cloacae Expressing Green Fluorescent Protein-Defensin (GFP-D) in Anopheles stephensi under Laboratory Condition |
| title_sort | dynamics of transgenic enterobacter cloacae expressing green fluorescent protein defensin gfp d in anopheles stephensi under laboratory condition |
| topic | Bacterial dynamic Enterobacter cloacae Anopheles stephensi Paratransgenesis |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/778 |
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