Embedded Living HER2+ Cells in a 3D Gelatin–Alginate Hydrogel as an In Vitro Model for Immunotherapy Delivery for Breast Cancer
Epidermal growth factor receptor 2 (HER2) is the second target molecule most commonly used in breast cancer treatment. Both recurrence and metastasis are still deadly for HER2+ breast cancer patients. Hydrogels can be an option for developing three-dimensional (3D) cell culture systems that resemble...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/18/3726 |
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| author | G. Tonantzin De Dios-Figueroa Janette del Rocío Aguilera-Márquez Lorena García-Uriostegui Rodolfo Hernández-Gutiérrez Tanya A. Camacho-Villegas Pavel H. Lugo-Fabres |
| author_facet | G. Tonantzin De Dios-Figueroa Janette del Rocío Aguilera-Márquez Lorena García-Uriostegui Rodolfo Hernández-Gutiérrez Tanya A. Camacho-Villegas Pavel H. Lugo-Fabres |
| author_sort | G. Tonantzin De Dios-Figueroa |
| collection | DOAJ |
| description | Epidermal growth factor receptor 2 (HER2) is the second target molecule most commonly used in breast cancer treatment. Both recurrence and metastasis are still deadly for HER2+ breast cancer patients. Hydrogels can be an option for developing three-dimensional (3D) cell culture systems that resemble tumor features better than monolayer cultures and could be used for preclinical screening for new biotherapeutics. Biopolymers (gelatin and alginate) were used to develop a hydrogel capable of encapsulating living HER2+ breast cancer cells BT-474/GFP. The hydrogel was physicochemically characterized, and the viability of embedded cells was evaluated. The hydrogel developed had suitable physical properties, with swelling of 38% of its original mass at 20 h capacity and pore sizes between 20 and 125 µm that allowed cells to maintain their morphology in a 3D environment, in addition to being biocompatible and preserving 90% of cell viability at 10 days. Furthermore, encapsulated BT-474/GFP cells maintained HER2 expression that could be detected by the Trastuzumab-fluorescent antibody, so this hydrogel could be used to evaluate new HER2-targeted therapies. |
| first_indexed | 2024-03-10T22:11:21Z |
| format | Article |
| id | doaj.art-65107e9466644b24ac6d7ddd5a89be25 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T22:11:21Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-65107e9466644b24ac6d7ddd5a89be252023-11-19T12:34:56ZengMDPI AGPolymers2073-43602023-09-011518372610.3390/polym15183726Embedded Living HER2+ Cells in a 3D Gelatin–Alginate Hydrogel as an In Vitro Model for Immunotherapy Delivery for Breast CancerG. Tonantzin De Dios-Figueroa0Janette del Rocío Aguilera-Márquez1Lorena García-Uriostegui2Rodolfo Hernández-Gutiérrez3Tanya A. Camacho-Villegas4Pavel H. Lugo-Fabres5Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara 44270, Jalisco, MexicoUnidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara 44270, Jalisco, MexicoCONAHCYT—Departamento de Madera, Celulosa y Papel, Universidad de Guadalajara (UDG), Guadalajara 44100, Jalisco, MexicoUnidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara 44270, Jalisco, MexicoCONAHCYT—Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara 44270, Jalisco, MexicoCONAHCYT—Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Guadalajara 44270, Jalisco, MexicoEpidermal growth factor receptor 2 (HER2) is the second target molecule most commonly used in breast cancer treatment. Both recurrence and metastasis are still deadly for HER2+ breast cancer patients. Hydrogels can be an option for developing three-dimensional (3D) cell culture systems that resemble tumor features better than monolayer cultures and could be used for preclinical screening for new biotherapeutics. Biopolymers (gelatin and alginate) were used to develop a hydrogel capable of encapsulating living HER2+ breast cancer cells BT-474/GFP. The hydrogel was physicochemically characterized, and the viability of embedded cells was evaluated. The hydrogel developed had suitable physical properties, with swelling of 38% of its original mass at 20 h capacity and pore sizes between 20 and 125 µm that allowed cells to maintain their morphology in a 3D environment, in addition to being biocompatible and preserving 90% of cell viability at 10 days. Furthermore, encapsulated BT-474/GFP cells maintained HER2 expression that could be detected by the Trastuzumab-fluorescent antibody, so this hydrogel could be used to evaluate new HER2-targeted therapies.https://www.mdpi.com/2073-4360/15/18/3726hydrogelthree-dimensional (3D) cell cultureliving embedded cellsbreast cancerHER2+ |
| spellingShingle | G. Tonantzin De Dios-Figueroa Janette del Rocío Aguilera-Márquez Lorena García-Uriostegui Rodolfo Hernández-Gutiérrez Tanya A. Camacho-Villegas Pavel H. Lugo-Fabres Embedded Living HER2+ Cells in a 3D Gelatin–Alginate Hydrogel as an In Vitro Model for Immunotherapy Delivery for Breast Cancer Polymers hydrogel three-dimensional (3D) cell culture living embedded cells breast cancer HER2+ |
| title | Embedded Living HER2+ Cells in a 3D Gelatin–Alginate Hydrogel as an In Vitro Model for Immunotherapy Delivery for Breast Cancer |
| title_full | Embedded Living HER2+ Cells in a 3D Gelatin–Alginate Hydrogel as an In Vitro Model for Immunotherapy Delivery for Breast Cancer |
| title_fullStr | Embedded Living HER2+ Cells in a 3D Gelatin–Alginate Hydrogel as an In Vitro Model for Immunotherapy Delivery for Breast Cancer |
| title_full_unstemmed | Embedded Living HER2+ Cells in a 3D Gelatin–Alginate Hydrogel as an In Vitro Model for Immunotherapy Delivery for Breast Cancer |
| title_short | Embedded Living HER2+ Cells in a 3D Gelatin–Alginate Hydrogel as an In Vitro Model for Immunotherapy Delivery for Breast Cancer |
| title_sort | embedded living her2 cells in a 3d gelatin alginate hydrogel as an in vitro model for immunotherapy delivery for breast cancer |
| topic | hydrogel three-dimensional (3D) cell culture living embedded cells breast cancer HER2+ |
| url | https://www.mdpi.com/2073-4360/15/18/3726 |
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