Experimental Murine Models for Colorectal Cancer Research
Colorectal cancer (CRC) is the third most prevalent malignancy worldwide and in both sexes. Numerous animal models for CRC have been established to study its biology, namely carcinogen-induced models (CIMs) and genetically engineered mouse models (GEMMs). CIMs are valuable for assessing colitis-rela...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/15/9/2570 |
| _version_ | 1797602912458768384 |
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| author | Íris Neto João Rocha Maria Manuela Gaspar Catarina P. Reis |
| author_facet | Íris Neto João Rocha Maria Manuela Gaspar Catarina P. Reis |
| author_sort | Íris Neto |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is the third most prevalent malignancy worldwide and in both sexes. Numerous animal models for CRC have been established to study its biology, namely carcinogen-induced models (CIMs) and genetically engineered mouse models (GEMMs). CIMs are valuable for assessing colitis-related carcinogenesis and studying chemoprevention. On the other hand, CRC GEMMs have proven to be useful for evaluating the tumor microenvironment and systemic immune responses, which have contributed to the discovery of novel therapeutic approaches. Although metastatic disease can be induced by orthotopic injection of CRC cell lines, the resulting models are not representative of the full genetic diversity of the disease due to the limited number of cell lines suitable for this purpose. On the other hand, patient-derived xenografts (PDX) are the most reliable for preclinical drug development due to their ability to retain pathological and molecular characteristics. In this review, the authors discuss the various murine CRC models with a focus on their clinical relevance, benefits, and drawbacks. From all models discussed, murine CRC models will continue to be an important tool in advancing our understanding and treatment of this disease, but additional research is required to find a model that can correctly reflect the pathophysiology of CRC. |
| first_indexed | 2024-03-11T04:22:14Z |
| format | Article |
| id | doaj.art-6511c2e5b00347279de5028c824f3aac |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T04:22:14Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-6511c2e5b00347279de5028c824f3aac2023-11-17T22:41:34ZengMDPI AGCancers2072-66942023-04-01159257010.3390/cancers15092570Experimental Murine Models for Colorectal Cancer ResearchÍris Neto0João Rocha1Maria Manuela Gaspar2Catarina P. Reis3Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugalResearch Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugalColorectal cancer (CRC) is the third most prevalent malignancy worldwide and in both sexes. Numerous animal models for CRC have been established to study its biology, namely carcinogen-induced models (CIMs) and genetically engineered mouse models (GEMMs). CIMs are valuable for assessing colitis-related carcinogenesis and studying chemoprevention. On the other hand, CRC GEMMs have proven to be useful for evaluating the tumor microenvironment and systemic immune responses, which have contributed to the discovery of novel therapeutic approaches. Although metastatic disease can be induced by orthotopic injection of CRC cell lines, the resulting models are not representative of the full genetic diversity of the disease due to the limited number of cell lines suitable for this purpose. On the other hand, patient-derived xenografts (PDX) are the most reliable for preclinical drug development due to their ability to retain pathological and molecular characteristics. In this review, the authors discuss the various murine CRC models with a focus on their clinical relevance, benefits, and drawbacks. From all models discussed, murine CRC models will continue to be an important tool in advancing our understanding and treatment of this disease, but additional research is required to find a model that can correctly reflect the pathophysiology of CRC.https://www.mdpi.com/2072-6694/15/9/2570murine modelcolorectal canceranimal modelpatient-derived xenograftscarcinogen-induced modelsgenetically engineered mouse models |
| spellingShingle | Íris Neto João Rocha Maria Manuela Gaspar Catarina P. Reis Experimental Murine Models for Colorectal Cancer Research Cancers murine model colorectal cancer animal model patient-derived xenografts carcinogen-induced models genetically engineered mouse models |
| title | Experimental Murine Models for Colorectal Cancer Research |
| title_full | Experimental Murine Models for Colorectal Cancer Research |
| title_fullStr | Experimental Murine Models for Colorectal Cancer Research |
| title_full_unstemmed | Experimental Murine Models for Colorectal Cancer Research |
| title_short | Experimental Murine Models for Colorectal Cancer Research |
| title_sort | experimental murine models for colorectal cancer research |
| topic | murine model colorectal cancer animal model patient-derived xenografts carcinogen-induced models genetically engineered mouse models |
| url | https://www.mdpi.com/2072-6694/15/9/2570 |
| work_keys_str_mv | AT irisneto experimentalmurinemodelsforcolorectalcancerresearch AT joaorocha experimentalmurinemodelsforcolorectalcancerresearch AT mariamanuelagaspar experimentalmurinemodelsforcolorectalcancerresearch AT catarinapreis experimentalmurinemodelsforcolorectalcancerresearch |