Decreased expression of CLCA2 and the correlating with immune infiltrates in patients with cervical squamous cell carcinoma: A bioinformatics analysis
Objective: Calcium-activated chloride channel 2 (CLCA2) is closely related to the invasion, metastasis, and prognosis of some common malignant tumors. The present study aimed to evaluate the role of CLCA2 in cervical squamous cell carcinoma (CESC) using bioinformatics analysis. Materials and methods...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-05-01
|
| Series: | Taiwanese Journal of Obstetrics & Gynecology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S102845592100067X |
| _version_ | 1818572402796265472 |
|---|---|
| author | Xin Yang Jin-Long Cao Feng-Na Yang Xiao-Feng Li Li-Mei Tao Fang Wang |
| author_facet | Xin Yang Jin-Long Cao Feng-Na Yang Xiao-Feng Li Li-Mei Tao Fang Wang |
| author_sort | Xin Yang |
| collection | DOAJ |
| description | Objective: Calcium-activated chloride channel 2 (CLCA2) is closely related to the invasion, metastasis, and prognosis of some common malignant tumors. The present study aimed to evaluate the role of CLCA2 in cervical squamous cell carcinoma (CESC) using bioinformatics analysis. Materials and methods: The mRNA sequencing data and the corresponding clinical data were obtained from Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA) database respectively. Then univariate analysis of variance was used to analyze the differential mRNA expression of CLCA2 between normal, cervical Intraepithelial neoplasia (CIN), and CESC tissues and clinicopathological characteristics. The Gene Expression Profiling Interactive Analysis (GEPIA) was used to assess the association between CLCA2 and Disease-Free Survival (DFS), overall survival (OS). The Gene Set Enrichment Analysis (GSEA) was used to explore the associated signaling pathways. The Tumor Immune Estimation Resource (TIMER) was used to predict the potential biological roles of CLCA2 in tumor-immune of CESC. Results: CLCA2 expression was significantly decreased in CESC tissues compared with normal and CIN tissues (P < 0.05). Meanwhile, obese patients had lower levels of CLCA2 expression than normal-weight CESC patients (P < 0.05). However, there was no significant difference in the expression level of CLCA2 in patients with different T stage, lymph node status, metastasis, and FIGO stage in CC(P > 0.05). The survival analysis indicated that for DFS, CESC with high CLCA2 expression was associated with better prognoses compared with those with low expression levels (P < 0.05). But for the OS, there was no difference. GSEA revealed that 4 pathways exhibited significant differential enrichment in the CLCA2 high-expression phenotype, including the P53 signaling pathway, the ERBB signaling pathway, the NOTCH signaling pathway, and the ubiquitin-mediated proteolysis. The TIMER reveals the expression of CLCA2 showed a significant inverse association with the number of B cells, Macrophage cells, and Dendritic Cell infiltration. Conclusion: The present study indicates that CLCA2 expression may be a potential prognostic marker for patients with CESC. |
| first_indexed | 2024-12-14T18:56:36Z |
| format | Article |
| id | doaj.art-65141845c3c2434293aec2631ebdf8bc |
| institution | Directory Open Access Journal |
| issn | 1028-4559 |
| language | English |
| last_indexed | 2024-12-14T18:56:36Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Taiwanese Journal of Obstetrics & Gynecology |
| spelling | doaj.art-65141845c3c2434293aec2631ebdf8bc2022-12-21T22:51:04ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592021-05-01603480486Decreased expression of CLCA2 and the correlating with immune infiltrates in patients with cervical squamous cell carcinoma: A bioinformatics analysisXin Yang0Jin-Long Cao1Feng-Na Yang2Xiao-Feng Li3Li-Mei Tao4Fang Wang5Lanzhou University Second Hospital, Lanzhou, ChinaLanzhou University Second Hospital, Lanzhou, ChinaLanzhou University Second Hospital, Lanzhou, ChinaLanzhou University Second Hospital, Lanzhou, ChinaLanzhou University Second Hospital, Lanzhou, ChinaCorresponding author. No.82, Cuiying Road, Chengguan District, Lanzhou, Gansu, China.; Lanzhou University Second Hospital, Lanzhou, ChinaObjective: Calcium-activated chloride channel 2 (CLCA2) is closely related to the invasion, metastasis, and prognosis of some common malignant tumors. The present study aimed to evaluate the role of CLCA2 in cervical squamous cell carcinoma (CESC) using bioinformatics analysis. Materials and methods: The mRNA sequencing data and the corresponding clinical data were obtained from Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA) database respectively. Then univariate analysis of variance was used to analyze the differential mRNA expression of CLCA2 between normal, cervical Intraepithelial neoplasia (CIN), and CESC tissues and clinicopathological characteristics. The Gene Expression Profiling Interactive Analysis (GEPIA) was used to assess the association between CLCA2 and Disease-Free Survival (DFS), overall survival (OS). The Gene Set Enrichment Analysis (GSEA) was used to explore the associated signaling pathways. The Tumor Immune Estimation Resource (TIMER) was used to predict the potential biological roles of CLCA2 in tumor-immune of CESC. Results: CLCA2 expression was significantly decreased in CESC tissues compared with normal and CIN tissues (P < 0.05). Meanwhile, obese patients had lower levels of CLCA2 expression than normal-weight CESC patients (P < 0.05). However, there was no significant difference in the expression level of CLCA2 in patients with different T stage, lymph node status, metastasis, and FIGO stage in CC(P > 0.05). The survival analysis indicated that for DFS, CESC with high CLCA2 expression was associated with better prognoses compared with those with low expression levels (P < 0.05). But for the OS, there was no difference. GSEA revealed that 4 pathways exhibited significant differential enrichment in the CLCA2 high-expression phenotype, including the P53 signaling pathway, the ERBB signaling pathway, the NOTCH signaling pathway, and the ubiquitin-mediated proteolysis. The TIMER reveals the expression of CLCA2 showed a significant inverse association with the number of B cells, Macrophage cells, and Dendritic Cell infiltration. Conclusion: The present study indicates that CLCA2 expression may be a potential prognostic marker for patients with CESC.http://www.sciencedirect.com/science/article/pii/S102845592100067XBioinformatics analysisCervical squamous cell carcinoma (CESC)Calcium-activated chloride channel 2 (CLCA2)GEO databaseTCGA database |
| spellingShingle | Xin Yang Jin-Long Cao Feng-Na Yang Xiao-Feng Li Li-Mei Tao Fang Wang Decreased expression of CLCA2 and the correlating with immune infiltrates in patients with cervical squamous cell carcinoma: A bioinformatics analysis Taiwanese Journal of Obstetrics & Gynecology Bioinformatics analysis Cervical squamous cell carcinoma (CESC) Calcium-activated chloride channel 2 (CLCA2) GEO database TCGA database |
| title | Decreased expression of CLCA2 and the correlating with immune infiltrates in patients with cervical squamous cell carcinoma: A bioinformatics analysis |
| title_full | Decreased expression of CLCA2 and the correlating with immune infiltrates in patients with cervical squamous cell carcinoma: A bioinformatics analysis |
| title_fullStr | Decreased expression of CLCA2 and the correlating with immune infiltrates in patients with cervical squamous cell carcinoma: A bioinformatics analysis |
| title_full_unstemmed | Decreased expression of CLCA2 and the correlating with immune infiltrates in patients with cervical squamous cell carcinoma: A bioinformatics analysis |
| title_short | Decreased expression of CLCA2 and the correlating with immune infiltrates in patients with cervical squamous cell carcinoma: A bioinformatics analysis |
| title_sort | decreased expression of clca2 and the correlating with immune infiltrates in patients with cervical squamous cell carcinoma a bioinformatics analysis |
| topic | Bioinformatics analysis Cervical squamous cell carcinoma (CESC) Calcium-activated chloride channel 2 (CLCA2) GEO database TCGA database |
| url | http://www.sciencedirect.com/science/article/pii/S102845592100067X |
| work_keys_str_mv | AT xinyang decreasedexpressionofclca2andthecorrelatingwithimmuneinfiltratesinpatientswithcervicalsquamouscellcarcinomaabioinformaticsanalysis AT jinlongcao decreasedexpressionofclca2andthecorrelatingwithimmuneinfiltratesinpatientswithcervicalsquamouscellcarcinomaabioinformaticsanalysis AT fengnayang decreasedexpressionofclca2andthecorrelatingwithimmuneinfiltratesinpatientswithcervicalsquamouscellcarcinomaabioinformaticsanalysis AT xiaofengli decreasedexpressionofclca2andthecorrelatingwithimmuneinfiltratesinpatientswithcervicalsquamouscellcarcinomaabioinformaticsanalysis AT limeitao decreasedexpressionofclca2andthecorrelatingwithimmuneinfiltratesinpatientswithcervicalsquamouscellcarcinomaabioinformaticsanalysis AT fangwang decreasedexpressionofclca2andthecorrelatingwithimmuneinfiltratesinpatientswithcervicalsquamouscellcarcinomaabioinformaticsanalysis |