Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa
Abstract Background The selection and the spread of insecticide resistance in malaria vectors to the main classes of insecticides used in vector control tools are a major and ongoing challenge to malaria vector control programmes. This study aimed to determine the intensity of vector resistance to i...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-07-01
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| Series: | Malaria Journal |
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| Online Access: | https://doi.org/10.1186/s12936-021-03856-2 |
| _version_ | 1828914643614040064 |
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| author | Casimir Dossou Kpanou Hermann W. Sagbohan Fortuné Dagnon Germain G. Padonou Razaki Ossè Albert Sourou Salako Aboubakar Sidick Wilfried Sewadé André Sominahouin Patrick Condo Saadani Hassani Ahmed Daniel Impoinvil Martin Akogbéto |
| author_facet | Casimir Dossou Kpanou Hermann W. Sagbohan Fortuné Dagnon Germain G. Padonou Razaki Ossè Albert Sourou Salako Aboubakar Sidick Wilfried Sewadé André Sominahouin Patrick Condo Saadani Hassani Ahmed Daniel Impoinvil Martin Akogbéto |
| author_sort | Casimir Dossou Kpanou |
| collection | DOAJ |
| description | Abstract Background The selection and the spread of insecticide resistance in malaria vectors to the main classes of insecticides used in vector control tools are a major and ongoing challenge to malaria vector control programmes. This study aimed to determine the intensity of vector resistance to insecticides in three regions of Benin with different agro-ecological characteristics. Methods Larvae of Anopheles gambiae sensu lato (s.l.) were collected from September to November 2017 in different larval sites in three northern Benin communes: Parakou, Kandi and Malanville. Two to five-day-old, non-blood-fed, female mosquitoes were exposed to papers impregnated with deltamethrin, permethrin and bendiocarb at dosages of 1 × the diagnostic dose, 5 × and 10 × to determine the intensity of resistance in these vectors. Molecular frequencies of the kdr L1014F and ace-1R G119S insecticide resistance mutations and levels of detoxification enzymes were determined for mosquitoes sampled at each study site. Results Resistance to pyrethroids (permethrin and deltamethrin) was recorded in all three communes with mortality rates below 60% using the diagnostic dose (1x). The results obtained after exposure of An. gambiae to permethrin 10 × were 99% in Kandi, 98% in Malanville and 99% in Parakou. With deltamethrin 10x, mortality rates were 100% in Kandi, 96% in Malanville and 73% in Parakou. For the diagnostic dose of bendiocarb, suspected resistance was recorded in the communes of Malanville (97%) and Kandi (94%) while sensitivity was observed in Parakou (98%).Using the 10 × dose, mortality was 98% in Kandi, 100% in Malanville and 99% in Parakou. The frequencies of the kdr L1014F allele varied between 59 and 83% depending on the sites and species of the An. gambiae complex, while the frequency of the ace-1R G119S gene varied between 0 and 5%. Biochemical tests showed high levels of oxidase and esterase activity compared to the susceptible colony strain of An. gambiae sensu stricto (Kisumu strain). Conclusion Anopheles gambiae showed a generalized loss of susceptibility to permethrin and deltamethrin but also showed moderate to high intensity of resistance in different regions of Benin. This high intensity of resistance is a potential threat to the effectiveness of vector control. |
| first_indexed | 2024-12-13T19:59:28Z |
| format | Article |
| id | doaj.art-6514bdbc883646c9a25fcc4efa04a44b |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-13T19:59:28Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
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| series | Malaria Journal |
| spelling | doaj.art-6514bdbc883646c9a25fcc4efa04a44b2022-12-21T23:33:14ZengBMCMalaria Journal1475-28752021-07-0120111210.1186/s12936-021-03856-2Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West AfricaCasimir Dossou Kpanou0Hermann W. Sagbohan1Fortuné Dagnon2Germain G. Padonou3Razaki Ossè4Albert Sourou Salako5Aboubakar Sidick6Wilfried Sewadé7André Sominahouin8Patrick Condo9Saadani Hassani Ahmed10Daniel Impoinvil11Martin Akogbéto12Centre de Recherche entomologique de Cotonou (CREC)Centre de Recherche entomologique de Cotonou (CREC)US President’s Malaria Initiative, US Agency for International DevelopmentCentre de Recherche entomologique de Cotonou (CREC)Centre de Recherche entomologique de Cotonou (CREC)Centre de Recherche entomologique de Cotonou (CREC)Centre de Recherche entomologique de Cotonou (CREC)Centre de Recherche entomologique de Cotonou (CREC)Centre de Recherche entomologique de Cotonou (CREC)US President’s Malaria Initiative, US Agency for International DevelopmentUS President’s Malaria Initiative, US Agency for International DevelopmentUS President’s Malaria Initiative, Centers for Disease Control and Prevention for Disease Control (CDC)Centre de Recherche entomologique de Cotonou (CREC)Abstract Background The selection and the spread of insecticide resistance in malaria vectors to the main classes of insecticides used in vector control tools are a major and ongoing challenge to malaria vector control programmes. This study aimed to determine the intensity of vector resistance to insecticides in three regions of Benin with different agro-ecological characteristics. Methods Larvae of Anopheles gambiae sensu lato (s.l.) were collected from September to November 2017 in different larval sites in three northern Benin communes: Parakou, Kandi and Malanville. Two to five-day-old, non-blood-fed, female mosquitoes were exposed to papers impregnated with deltamethrin, permethrin and bendiocarb at dosages of 1 × the diagnostic dose, 5 × and 10 × to determine the intensity of resistance in these vectors. Molecular frequencies of the kdr L1014F and ace-1R G119S insecticide resistance mutations and levels of detoxification enzymes were determined for mosquitoes sampled at each study site. Results Resistance to pyrethroids (permethrin and deltamethrin) was recorded in all three communes with mortality rates below 60% using the diagnostic dose (1x). The results obtained after exposure of An. gambiae to permethrin 10 × were 99% in Kandi, 98% in Malanville and 99% in Parakou. With deltamethrin 10x, mortality rates were 100% in Kandi, 96% in Malanville and 73% in Parakou. For the diagnostic dose of bendiocarb, suspected resistance was recorded in the communes of Malanville (97%) and Kandi (94%) while sensitivity was observed in Parakou (98%).Using the 10 × dose, mortality was 98% in Kandi, 100% in Malanville and 99% in Parakou. The frequencies of the kdr L1014F allele varied between 59 and 83% depending on the sites and species of the An. gambiae complex, while the frequency of the ace-1R G119S gene varied between 0 and 5%. Biochemical tests showed high levels of oxidase and esterase activity compared to the susceptible colony strain of An. gambiae sensu stricto (Kisumu strain). Conclusion Anopheles gambiae showed a generalized loss of susceptibility to permethrin and deltamethrin but also showed moderate to high intensity of resistance in different regions of Benin. This high intensity of resistance is a potential threat to the effectiveness of vector control.https://doi.org/10.1186/s12936-021-03856-2Insecticide resistanceIntensityAnopheles gambiaePyrethroidsBendiocarb |
| spellingShingle | Casimir Dossou Kpanou Hermann W. Sagbohan Fortuné Dagnon Germain G. Padonou Razaki Ossè Albert Sourou Salako Aboubakar Sidick Wilfried Sewadé André Sominahouin Patrick Condo Saadani Hassani Ahmed Daniel Impoinvil Martin Akogbéto Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa Malaria Journal Insecticide resistance Intensity Anopheles gambiae Pyrethroids Bendiocarb |
| title | Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
| title_full | Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
| title_fullStr | Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
| title_full_unstemmed | Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
| title_short | Characterization of resistance profile (intensity and mechanisms) of Anopheles gambiae in three communes of northern Benin, West Africa |
| title_sort | characterization of resistance profile intensity and mechanisms of anopheles gambiae in three communes of northern benin west africa |
| topic | Insecticide resistance Intensity Anopheles gambiae Pyrethroids Bendiocarb |
| url | https://doi.org/10.1186/s12936-021-03856-2 |
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