Description of Antimicrobial-Resistant <i>Escherichia coli</i> and Their Dissemination Mechanisms on Dairy Farms
Despite its importance in veterinary medicine, there is little information about antimicrobial resistance (AMR) and its transmission in dairy cattle. The aim of this work is to compare AMR phenotypes and genotypes in resistant <i>Escherichia coli</i> and to determine how the resistance g...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/4/242 |
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| author | Jonathan Massé Ghyslaine Vanier John M. Fairbrother Maud de Lagarde Julie Arsenault David Francoz Simon Dufour Marie Archambault |
| author_facet | Jonathan Massé Ghyslaine Vanier John M. Fairbrother Maud de Lagarde Julie Arsenault David Francoz Simon Dufour Marie Archambault |
| author_sort | Jonathan Massé |
| collection | DOAJ |
| description | Despite its importance in veterinary medicine, there is little information about antimicrobial resistance (AMR) and its transmission in dairy cattle. The aim of this work is to compare AMR phenotypes and genotypes in resistant <i>Escherichia coli</i> and to determine how the resistance genes spread among the <i>E. coli</i> population on dairy farms in Québec, Canada. From an existing culture collection of <i>E. coli</i> isolated from dairy manure, a convenient selection of the most resistant isolates (a high level of multidrug resistance or resistance to broad-spectrum β-lactams or fluoroquinolones) was analyzed (<i>n</i> = 118). An AMR phenotype profile was obtained for each isolate. Whole genome sequencing was used to determine the presence of resistance genes, point mutations, and mobile genetic elements. In addition, a subset of isolates from 86 farms was taken to investigate the phylogenetic relationship and geographic distribution of the isolates. The average agreement between AMR phenotypes and genotypes was 95%. A third-generation cephalosporin resistance gene (<i>bla<sub>CTX-M-15</sub></i>), a resistance gene conferring reduced susceptibility to fluoroquinolones (<i>qnrS</i>1), and an insertion sequence (ISKpn19) were detected in the vicinity of each other on the genome. These genes were harbored in one triplet of clonal isolates from three farms located >100 km apart. Our study reveals the dissemination of resistant <i>E. coli</i> clones between dairy farms. Furthermore, these clones are resistant to broad-spectrum β-lactam and fluoroquinolone antimicrobials. |
| first_indexed | 2024-03-11T04:27:08Z |
| format | Article |
| id | doaj.art-651f931ed9ed4c8099f441893525169c |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T04:27:08Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-651f931ed9ed4c8099f441893525169c2023-11-17T21:43:09ZengMDPI AGVeterinary Sciences2306-73812023-03-0110424210.3390/vetsci10040242Description of Antimicrobial-Resistant <i>Escherichia coli</i> and Their Dissemination Mechanisms on Dairy FarmsJonathan Massé0Ghyslaine Vanier1John M. Fairbrother2Maud de Lagarde3Julie Arsenault4David Francoz5Simon Dufour6Marie Archambault7Regroupement FRQNT Op+lait, Saint-Hyacinthe, QC J2S 2M2, CanadaGroupe de Recherche sur les Maladies Infectieuses en Production Animale (GREMIP), Saint-Hyacinthe, QC J2S 2M2, CanadaGroupe de Recherche sur les Maladies Infectieuses en Production Animale (GREMIP), Saint-Hyacinthe, QC J2S 2M2, CanadaRegroupement FRQNT Op+lait, Saint-Hyacinthe, QC J2S 2M2, CanadaDepartment of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, CanadaRegroupement FRQNT Op+lait, Saint-Hyacinthe, QC J2S 2M2, CanadaRegroupement FRQNT Op+lait, Saint-Hyacinthe, QC J2S 2M2, CanadaRegroupement FRQNT Op+lait, Saint-Hyacinthe, QC J2S 2M2, CanadaDespite its importance in veterinary medicine, there is little information about antimicrobial resistance (AMR) and its transmission in dairy cattle. The aim of this work is to compare AMR phenotypes and genotypes in resistant <i>Escherichia coli</i> and to determine how the resistance genes spread among the <i>E. coli</i> population on dairy farms in Québec, Canada. From an existing culture collection of <i>E. coli</i> isolated from dairy manure, a convenient selection of the most resistant isolates (a high level of multidrug resistance or resistance to broad-spectrum β-lactams or fluoroquinolones) was analyzed (<i>n</i> = 118). An AMR phenotype profile was obtained for each isolate. Whole genome sequencing was used to determine the presence of resistance genes, point mutations, and mobile genetic elements. In addition, a subset of isolates from 86 farms was taken to investigate the phylogenetic relationship and geographic distribution of the isolates. The average agreement between AMR phenotypes and genotypes was 95%. A third-generation cephalosporin resistance gene (<i>bla<sub>CTX-M-15</sub></i>), a resistance gene conferring reduced susceptibility to fluoroquinolones (<i>qnrS</i>1), and an insertion sequence (ISKpn19) were detected in the vicinity of each other on the genome. These genes were harbored in one triplet of clonal isolates from three farms located >100 km apart. Our study reveals the dissemination of resistant <i>E. coli</i> clones between dairy farms. Furthermore, these clones are resistant to broad-spectrum β-lactam and fluoroquinolone antimicrobials.https://www.mdpi.com/2306-7381/10/4/242antimicrobial resistancewhole genome sequencingdairy cattlefluoroquinolonethird-generation cephalosporinmultidrug resistance |
| spellingShingle | Jonathan Massé Ghyslaine Vanier John M. Fairbrother Maud de Lagarde Julie Arsenault David Francoz Simon Dufour Marie Archambault Description of Antimicrobial-Resistant <i>Escherichia coli</i> and Their Dissemination Mechanisms on Dairy Farms Veterinary Sciences antimicrobial resistance whole genome sequencing dairy cattle fluoroquinolone third-generation cephalosporin multidrug resistance |
| title | Description of Antimicrobial-Resistant <i>Escherichia coli</i> and Their Dissemination Mechanisms on Dairy Farms |
| title_full | Description of Antimicrobial-Resistant <i>Escherichia coli</i> and Their Dissemination Mechanisms on Dairy Farms |
| title_fullStr | Description of Antimicrobial-Resistant <i>Escherichia coli</i> and Their Dissemination Mechanisms on Dairy Farms |
| title_full_unstemmed | Description of Antimicrobial-Resistant <i>Escherichia coli</i> and Their Dissemination Mechanisms on Dairy Farms |
| title_short | Description of Antimicrobial-Resistant <i>Escherichia coli</i> and Their Dissemination Mechanisms on Dairy Farms |
| title_sort | description of antimicrobial resistant i escherichia coli i and their dissemination mechanisms on dairy farms |
| topic | antimicrobial resistance whole genome sequencing dairy cattle fluoroquinolone third-generation cephalosporin multidrug resistance |
| url | https://www.mdpi.com/2306-7381/10/4/242 |
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