The evolution of advanced molecular diagnostics for the detection and characterization of Mycoplasma pneumoniae
Over the past several years there have been significant advancements in the methods used for detecting and characterizing Mycoplasma pneumoniae, a common cause of respiratory illness and community-acquired pneumonia worldwide. The repertoire of available molecular diagnostics has greatly expanded fr...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-03-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00232/full |
| _version_ | 1818518467731521536 |
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| author | Maureen H. Diaz Jonas M. Winchell |
| author_facet | Maureen H. Diaz Jonas M. Winchell |
| author_sort | Maureen H. Diaz |
| collection | DOAJ |
| description | Over the past several years there have been significant advancements in the methods used for detecting and characterizing Mycoplasma pneumoniae, a common cause of respiratory illness and community-acquired pneumonia worldwide. The repertoire of available molecular diagnostics has greatly expanded from nucleic acid amplification techniques (NAATs) that encompass a variety of chemistries used for detection, to more sophisticated characterizing methods such as multi-locus variable-number tandem-repeat analysis and sequencing typing (MLVA and MLST, respectively), matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS), single nucleotide polymorphism (SNP) typing, and numerous macrolide susceptibility profiling methods, among others. These many molecular-based approaches have been developed and employed to continually increase the level of discrimination and characterization in order to better understand the epidemiology and biology of M. pneumoniae. This review will summarize recent molecular techniques and procedures and lend perspective to how each has enhanced the current understanding of this organism and will emphasize how Next Generation Sequencing may serve as a resource for researchers to gain a more comprehensive understanding of the genomic complexities of this insidious pathogen. |
| first_indexed | 2024-12-11T01:10:44Z |
| format | Article |
| id | doaj.art-6520bfec66024bb1a7de7694a15c50e4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T01:10:44Z |
| publishDate | 2016-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6520bfec66024bb1a7de7694a15c50e42022-12-22T01:26:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-03-01710.3389/fmicb.2016.00232186106The evolution of advanced molecular diagnostics for the detection and characterization of Mycoplasma pneumoniaeMaureen H. Diaz0Jonas M. Winchell1Centers for Disease Control and PreventionCenters for Disease Control and PreventionOver the past several years there have been significant advancements in the methods used for detecting and characterizing Mycoplasma pneumoniae, a common cause of respiratory illness and community-acquired pneumonia worldwide. The repertoire of available molecular diagnostics has greatly expanded from nucleic acid amplification techniques (NAATs) that encompass a variety of chemistries used for detection, to more sophisticated characterizing methods such as multi-locus variable-number tandem-repeat analysis and sequencing typing (MLVA and MLST, respectively), matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS), single nucleotide polymorphism (SNP) typing, and numerous macrolide susceptibility profiling methods, among others. These many molecular-based approaches have been developed and employed to continually increase the level of discrimination and characterization in order to better understand the epidemiology and biology of M. pneumoniae. This review will summarize recent molecular techniques and procedures and lend perspective to how each has enhanced the current understanding of this organism and will emphasize how Next Generation Sequencing may serve as a resource for researchers to gain a more comprehensive understanding of the genomic complexities of this insidious pathogen.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00232/fullMolecular EpidemiologyMycoplasma pneumoniaeMolecular diagnosticsMolecular Characteristicswhole genome sequencing |
| spellingShingle | Maureen H. Diaz Jonas M. Winchell The evolution of advanced molecular diagnostics for the detection and characterization of Mycoplasma pneumoniae Frontiers in Microbiology Molecular Epidemiology Mycoplasma pneumoniae Molecular diagnostics Molecular Characteristics whole genome sequencing |
| title | The evolution of advanced molecular diagnostics for the detection and characterization of Mycoplasma pneumoniae |
| title_full | The evolution of advanced molecular diagnostics for the detection and characterization of Mycoplasma pneumoniae |
| title_fullStr | The evolution of advanced molecular diagnostics for the detection and characterization of Mycoplasma pneumoniae |
| title_full_unstemmed | The evolution of advanced molecular diagnostics for the detection and characterization of Mycoplasma pneumoniae |
| title_short | The evolution of advanced molecular diagnostics for the detection and characterization of Mycoplasma pneumoniae |
| title_sort | evolution of advanced molecular diagnostics for the detection and characterization of mycoplasma pneumoniae |
| topic | Molecular Epidemiology Mycoplasma pneumoniae Molecular diagnostics Molecular Characteristics whole genome sequencing |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00232/full |
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