Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review
Plant viral diseases are the foremost threat to sustainable agriculture, leading to several billion dollars in losses every year. Many viruses infecting several crops have been described in the literature; however, new infectious viruses are emerging frequently through outbreaks. For the effective t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/3/412 |
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| author | Gajanan T. Mehetre Vincent Vineeth Leo Garima Singh Antonina Sorokan Igor Maksimov Mukesh Kumar Yadav Kalidas Upadhyaya Abeer Hashem Asma N. Alsaleh Turki M. Dawoud Khalid S. Almaary Bhim Pratap Singh |
| author_facet | Gajanan T. Mehetre Vincent Vineeth Leo Garima Singh Antonina Sorokan Igor Maksimov Mukesh Kumar Yadav Kalidas Upadhyaya Abeer Hashem Asma N. Alsaleh Turki M. Dawoud Khalid S. Almaary Bhim Pratap Singh |
| author_sort | Gajanan T. Mehetre |
| collection | DOAJ |
| description | Plant viral diseases are the foremost threat to sustainable agriculture, leading to several billion dollars in losses every year. Many viruses infecting several crops have been described in the literature; however, new infectious viruses are emerging frequently through outbreaks. For the effective treatment and prevention of viral diseases, there is great demand for new techniques that can provide accurate identification on the causative agents. With the advancements in biochemical and molecular biology techniques, several diagnostic methods with improved sensitivity and specificity for the detection of prevalent and/or unknown plant viruses are being continuously developed. Currently, serological and nucleic acid methods are the most widely used for plant viral diagnosis. Nucleic acid-based techniques that amplify target DNA/RNA have been evolved with many variants. However, there is growing interest in developing techniques that can be based in real-time and thus facilitate in-field diagnosis. Next-generation sequencing (NGS)-based innovative methods have shown great potential to detect multiple viruses simultaneously; however, such techniques are in the preliminary stages in plant viral disease diagnostics. This review discusses the recent progress in the use of NGS-based techniques for the detection, diagnosis, and identification of plant viral diseases. New portable devices and technologies that could provide real-time analyses in a relatively short period of time are prime important for in-field diagnostics. Current development and application of such tools and techniques along with their potential limitations in plant virology are likewise discussed in detail. |
| first_indexed | 2024-03-09T05:24:18Z |
| format | Article |
| id | doaj.art-6521750abb014af29ffcb4ca5269d4c9 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T05:24:18Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-6521750abb014af29ffcb4ca5269d4c92023-12-03T12:38:12ZengMDPI AGViruses1999-49152021-03-0113341210.3390/v13030412Current Developments and Challenges in Plant Viral Diagnostics: A Systematic ReviewGajanan T. Mehetre0Vincent Vineeth Leo1Garima Singh2Antonina Sorokan3Igor Maksimov4Mukesh Kumar Yadav5Kalidas Upadhyaya6Abeer Hashem7Asma N. Alsaleh8Turki M. Dawoud9Khalid S. Almaary10Bhim Pratap Singh11Department of Biotechnology, Mizoram University, Aizawl, Mizoram 796004, IndiaDepartment of Biotechnology, Mizoram University, Aizawl, Mizoram 796004, IndiaDepartment of Botany, Pachhunga University College, Aizawl, Mizoram 796001, IndiaInstitute of Biochemistry and Genetics, Ufa Federal Research Center of the Russian Academy of Sciences, pr. Oktyabrya 71, 450054 Ufa, RussiaInstitute of Biochemistry and Genetics, Ufa Federal Research Center of the Russian Academy of Sciences, pr. Oktyabrya 71, 450054 Ufa, RussiaDepartment of Biotechnology, Pachhunga University College, Aizawl, Mizoram 796001, IndiaDepartment of Forestry, Mizoram University, Aizawl, Mizoram 796004, IndiaBotany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi ArabiaDepartment of Agriculture and Environmental Sciences, National Institute of Food Technology Entrepreneurship & Management (NIFTEM), Industrial Estate, Kundli 131028, IndiaPlant viral diseases are the foremost threat to sustainable agriculture, leading to several billion dollars in losses every year. Many viruses infecting several crops have been described in the literature; however, new infectious viruses are emerging frequently through outbreaks. For the effective treatment and prevention of viral diseases, there is great demand for new techniques that can provide accurate identification on the causative agents. With the advancements in biochemical and molecular biology techniques, several diagnostic methods with improved sensitivity and specificity for the detection of prevalent and/or unknown plant viruses are being continuously developed. Currently, serological and nucleic acid methods are the most widely used for plant viral diagnosis. Nucleic acid-based techniques that amplify target DNA/RNA have been evolved with many variants. However, there is growing interest in developing techniques that can be based in real-time and thus facilitate in-field diagnosis. Next-generation sequencing (NGS)-based innovative methods have shown great potential to detect multiple viruses simultaneously; however, such techniques are in the preliminary stages in plant viral disease diagnostics. This review discusses the recent progress in the use of NGS-based techniques for the detection, diagnosis, and identification of plant viral diseases. New portable devices and technologies that could provide real-time analyses in a relatively short period of time are prime important for in-field diagnostics. Current development and application of such tools and techniques along with their potential limitations in plant virology are likewise discussed in detail.https://www.mdpi.com/1999-4915/13/3/412plant virusesdiagnosticsnext-generation sequencingomics technologiesnanopore sequencingquasi-species |
| spellingShingle | Gajanan T. Mehetre Vincent Vineeth Leo Garima Singh Antonina Sorokan Igor Maksimov Mukesh Kumar Yadav Kalidas Upadhyaya Abeer Hashem Asma N. Alsaleh Turki M. Dawoud Khalid S. Almaary Bhim Pratap Singh Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review Viruses plant viruses diagnostics next-generation sequencing omics technologies nanopore sequencing quasi-species |
| title | Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review |
| title_full | Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review |
| title_fullStr | Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review |
| title_full_unstemmed | Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review |
| title_short | Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review |
| title_sort | current developments and challenges in plant viral diagnostics a systematic review |
| topic | plant viruses diagnostics next-generation sequencing omics technologies nanopore sequencing quasi-species |
| url | https://www.mdpi.com/1999-4915/13/3/412 |
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