Activation of telomerase activity and telomere elongation of host cells by Theileria annulata infection
Theileria annulata-transformed cells share many phenotypes with cancer cells, including uncontrolled proliferation, immortalization, and dissemination. Telomeres are DNA-protein complex at the end of eukaryotic chromosomes that function to maintain genome stability and cell replicative capacity. Tel...
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128433/full |
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| author | Junlong Liu Shuaiyang Zhao Zhi Li Zhigang Zhang Baocai Zhao Guiquan Guan Hong Yin Hong Yin Jianxun Luo |
| author_facet | Junlong Liu Shuaiyang Zhao Zhi Li Zhigang Zhang Baocai Zhao Guiquan Guan Hong Yin Hong Yin Jianxun Luo |
| author_sort | Junlong Liu |
| collection | DOAJ |
| description | Theileria annulata-transformed cells share many phenotypes with cancer cells, including uncontrolled proliferation, immortalization, and dissemination. Telomeres are DNA-protein complex at the end of eukaryotic chromosomes that function to maintain genome stability and cell replicative capacity. Telomere length maintenance is primarily dependent on telomerase activity. In up to 90% of human cancer cells, telomerase is reactivated through expression of its catalytic subunit TERT. However, the effect of T. annulata infection on telomere and telomerase activity in bovine cells has not yet been described. In the present study, we confirmed that telomere length and telomerase activity are upregulated after T. annulata infection in three types of cell lines. This change depends on the presence of parasites. After eliminating Theileria from cells with antitheilerial drug buparvaquone, telomerase activity and the expression level of bTERT were decreased. In addition, inhibition of bHSP90 by novobiocin led to decreased AKT phosphorylation levels and telomerase activity, indicating that the bHSP90-AKT complex is a potent factor modulates telomerase activity in T. annulata-infected cells. |
| first_indexed | 2024-04-10T07:54:13Z |
| format | Article |
| id | doaj.art-65218119d04b4c52b9de1cd70397f901 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T07:54:13Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-65218119d04b4c52b9de1cd70397f9012023-02-23T05:44:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.11284331128433Activation of telomerase activity and telomere elongation of host cells by Theileria annulata infectionJunlong Liu0Shuaiyang Zhao1Zhi Li2Zhigang Zhang3Baocai Zhao4Guiquan Guan5Hong Yin6Hong Yin7Jianxun Luo8State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, ChinaQinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Xining, Qinghai, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, ChinaJiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, ChinaTheileria annulata-transformed cells share many phenotypes with cancer cells, including uncontrolled proliferation, immortalization, and dissemination. Telomeres are DNA-protein complex at the end of eukaryotic chromosomes that function to maintain genome stability and cell replicative capacity. Telomere length maintenance is primarily dependent on telomerase activity. In up to 90% of human cancer cells, telomerase is reactivated through expression of its catalytic subunit TERT. However, the effect of T. annulata infection on telomere and telomerase activity in bovine cells has not yet been described. In the present study, we confirmed that telomere length and telomerase activity are upregulated after T. annulata infection in three types of cell lines. This change depends on the presence of parasites. After eliminating Theileria from cells with antitheilerial drug buparvaquone, telomerase activity and the expression level of bTERT were decreased. In addition, inhibition of bHSP90 by novobiocin led to decreased AKT phosphorylation levels and telomerase activity, indicating that the bHSP90-AKT complex is a potent factor modulates telomerase activity in T. annulata-infected cells.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128433/fullTheileria annulatahost celltelomeretelomerasebTERTbHSP90 |
| spellingShingle | Junlong Liu Shuaiyang Zhao Zhi Li Zhigang Zhang Baocai Zhao Guiquan Guan Hong Yin Hong Yin Jianxun Luo Activation of telomerase activity and telomere elongation of host cells by Theileria annulata infection Frontiers in Microbiology Theileria annulata host cell telomere telomerase bTERT bHSP90 |
| title | Activation of telomerase activity and telomere elongation of host cells by Theileria annulata infection |
| title_full | Activation of telomerase activity and telomere elongation of host cells by Theileria annulata infection |
| title_fullStr | Activation of telomerase activity and telomere elongation of host cells by Theileria annulata infection |
| title_full_unstemmed | Activation of telomerase activity and telomere elongation of host cells by Theileria annulata infection |
| title_short | Activation of telomerase activity and telomere elongation of host cells by Theileria annulata infection |
| title_sort | activation of telomerase activity and telomere elongation of host cells by theileria annulata infection |
| topic | Theileria annulata host cell telomere telomerase bTERT bHSP90 |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128433/full |
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