Comparative Evaluation of Three Immunoassays for the Simultaneous Detection of <i>Clostridioides difficile</i> Glutamate Dehydrogenase and Toxin A/B
Background: In the medical laboratory, a step-by-step workflow for <i>Clostridioides difficile</i> infection (CDI) detection using glutamate dehydrogenase (GDH) and toxin A/B assays for initial screening, along with a nucleic acid amplification test (NAAT), has been recommended recently....
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MDPI AG
2022-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/5/947 |
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| author | Namsu Kim Seung Yeob Lee Joonhong Park Jaehyeon Lee |
| author_facet | Namsu Kim Seung Yeob Lee Joonhong Park Jaehyeon Lee |
| author_sort | Namsu Kim |
| collection | DOAJ |
| description | Background: In the medical laboratory, a step-by-step workflow for <i>Clostridioides difficile</i> infection (CDI) detection using glutamate dehydrogenase (GDH) and toxin A/B assays for initial screening, along with a nucleic acid amplification test (NAAT), has been recommended recently. In this study, we evaluated these three immunoassays for the simultaneous detection of GDH and <i>Clostridioides difficile</i> (CD) toxin A/B. Methods: A total of 304 stool samples were tested for the presence of GDH antigen and CD toxin A/B using VIDAS <i>C. difficile</i> GDH and toxin A/B (CDAB), RIDASCREEN <i>C. difficile</i> GDH and toxin A/B (RIDA), and C. DIFF QUIK CHEK COMPLETE according to the manufacturers’ recommendations. As complementary reference methods for GDH and toxin A/B detection in the three immunoassays, CD cultures using ChromID <i>C. difficile</i> agar and the Xpert <i>C. difficile</i> assay, respectively, were tested. Results: All three GDH assays showed overall substantial agreement with the CD culture. All three toxin A/B assays showed overall moderate agreement with the Xpert <i>C. difficile</i> assay. In comparison with consensus results, VIDAS GDH and QCC GDH showed almost perfect agreement, whereas RIDA GDH showed inferior but substantial agreement. All three toxin A/B assays showed almost perfect agreement. Conclusions: Since the QCC GDH and toxin A/B assay is relatively more sensitive and specific than the other two immunoassays for discriminating toxigenic or non-toxigenic CDI, QCC is very helpful for the simultaneous identification of GDH and CD toxin A/B in the initial step of the two-round workflow for diagnosing CDI. |
| first_indexed | 2024-03-10T03:23:05Z |
| format | Article |
| id | doaj.art-6521f6054e754673983cf6baaef70704 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:23:05Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6521f6054e754673983cf6baaef707042023-11-23T12:15:26ZengMDPI AGMicroorganisms2076-26072022-04-0110594710.3390/microorganisms10050947Comparative Evaluation of Three Immunoassays for the Simultaneous Detection of <i>Clostridioides difficile</i> Glutamate Dehydrogenase and Toxin A/BNamsu Kim0Seung Yeob Lee1Joonhong Park2Jaehyeon Lee3Department of Laboratory Medicine, Jeonbuk National University Medical School and Hospital, Jeonju 54907, KoreaDepartment of Laboratory Medicine, Jeonbuk National University Medical School and Hospital, Jeonju 54907, KoreaDepartment of Laboratory Medicine, Jeonbuk National University Medical School and Hospital, Jeonju 54907, KoreaDepartment of Laboratory Medicine, Jeonbuk National University Medical School and Hospital, Jeonju 54907, KoreaBackground: In the medical laboratory, a step-by-step workflow for <i>Clostridioides difficile</i> infection (CDI) detection using glutamate dehydrogenase (GDH) and toxin A/B assays for initial screening, along with a nucleic acid amplification test (NAAT), has been recommended recently. In this study, we evaluated these three immunoassays for the simultaneous detection of GDH and <i>Clostridioides difficile</i> (CD) toxin A/B. Methods: A total of 304 stool samples were tested for the presence of GDH antigen and CD toxin A/B using VIDAS <i>C. difficile</i> GDH and toxin A/B (CDAB), RIDASCREEN <i>C. difficile</i> GDH and toxin A/B (RIDA), and C. DIFF QUIK CHEK COMPLETE according to the manufacturers’ recommendations. As complementary reference methods for GDH and toxin A/B detection in the three immunoassays, CD cultures using ChromID <i>C. difficile</i> agar and the Xpert <i>C. difficile</i> assay, respectively, were tested. Results: All three GDH assays showed overall substantial agreement with the CD culture. All three toxin A/B assays showed overall moderate agreement with the Xpert <i>C. difficile</i> assay. In comparison with consensus results, VIDAS GDH and QCC GDH showed almost perfect agreement, whereas RIDA GDH showed inferior but substantial agreement. All three toxin A/B assays showed almost perfect agreement. Conclusions: Since the QCC GDH and toxin A/B assay is relatively more sensitive and specific than the other two immunoassays for discriminating toxigenic or non-toxigenic CDI, QCC is very helpful for the simultaneous identification of GDH and CD toxin A/B in the initial step of the two-round workflow for diagnosing CDI.https://www.mdpi.com/2076-2607/10/5/947<i>Clostridioides difficile</i>glutamate dehydrogenaseCD toxin A/BVIDAS <i>C. difficile</i> GDHVIDAS CDABRIDASCREEN <i>C. difficile</i> GDH |
| spellingShingle | Namsu Kim Seung Yeob Lee Joonhong Park Jaehyeon Lee Comparative Evaluation of Three Immunoassays for the Simultaneous Detection of <i>Clostridioides difficile</i> Glutamate Dehydrogenase and Toxin A/B Microorganisms <i>Clostridioides difficile</i> glutamate dehydrogenase CD toxin A/B VIDAS <i>C. difficile</i> GDH VIDAS CDAB RIDASCREEN <i>C. difficile</i> GDH |
| title | Comparative Evaluation of Three Immunoassays for the Simultaneous Detection of <i>Clostridioides difficile</i> Glutamate Dehydrogenase and Toxin A/B |
| title_full | Comparative Evaluation of Three Immunoassays for the Simultaneous Detection of <i>Clostridioides difficile</i> Glutamate Dehydrogenase and Toxin A/B |
| title_fullStr | Comparative Evaluation of Three Immunoassays for the Simultaneous Detection of <i>Clostridioides difficile</i> Glutamate Dehydrogenase and Toxin A/B |
| title_full_unstemmed | Comparative Evaluation of Three Immunoassays for the Simultaneous Detection of <i>Clostridioides difficile</i> Glutamate Dehydrogenase and Toxin A/B |
| title_short | Comparative Evaluation of Three Immunoassays for the Simultaneous Detection of <i>Clostridioides difficile</i> Glutamate Dehydrogenase and Toxin A/B |
| title_sort | comparative evaluation of three immunoassays for the simultaneous detection of i clostridioides difficile i glutamate dehydrogenase and toxin a b |
| topic | <i>Clostridioides difficile</i> glutamate dehydrogenase CD toxin A/B VIDAS <i>C. difficile</i> GDH VIDAS CDAB RIDASCREEN <i>C. difficile</i> GDH |
| url | https://www.mdpi.com/2076-2607/10/5/947 |
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