PKC regulates the production of fibroblast growth factor 23 (FGF23).
Serine/threonine protein kinase C (PKC) is activated by diacylglycerol that is released from membrane lipids by phospholipase C in response to activation of G protein-coupled receptors or receptor tyrosine kinases. PKC isoforms are particularly relevant for proliferation and differentiation of cells...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0211309 |
| _version_ | 1818584165214322688 |
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| author | Ludmilla Bär Philipp Hase Michael Föller |
| author_facet | Ludmilla Bär Philipp Hase Michael Föller |
| author_sort | Ludmilla Bär |
| collection | DOAJ |
| description | Serine/threonine protein kinase C (PKC) is activated by diacylglycerol that is released from membrane lipids by phospholipase C in response to activation of G protein-coupled receptors or receptor tyrosine kinases. PKC isoforms are particularly relevant for proliferation and differentiation of cells including osteoblasts. Osteoblasts/osteocytes produce fibroblast growth factor 23 (FGF23), a hormone regulating renal phosphate and vitamin D handling. PKC activates NFκB, a transcription factor complex controlling FGF23 expression. Here, we analyzed the impact of PKC on FGF23 synthesis. Fgf23 expression was analyzed by qRT-PCR in UMR106 osteoblast-like cells and in IDG-SW3 osteocytes, and FGF23 protein was measured by ELISA. Phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA), a PKC activator, up-regulated FGF23 production. In contrast, PKC inhibitors calphostin C, Gö6976, sotrastaurin and ruboxistaurin suppressed FGF23 formation. NFκB inhibitor withaferin A abolished the stimulatory effect of PMA on Fgf23. PKC is a powerful regulator of FGF23 synthesis, an effect which is at least partly mediated by NFκB. |
| first_indexed | 2024-12-16T08:16:50Z |
| format | Article |
| id | doaj.art-65228134de924cf4ac2bd7da60343e53 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:16:50Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-65228134de924cf4ac2bd7da60343e532022-12-21T22:38:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01143e021130910.1371/journal.pone.0211309PKC regulates the production of fibroblast growth factor 23 (FGF23).Ludmilla BärPhilipp HaseMichael FöllerSerine/threonine protein kinase C (PKC) is activated by diacylglycerol that is released from membrane lipids by phospholipase C in response to activation of G protein-coupled receptors or receptor tyrosine kinases. PKC isoforms are particularly relevant for proliferation and differentiation of cells including osteoblasts. Osteoblasts/osteocytes produce fibroblast growth factor 23 (FGF23), a hormone regulating renal phosphate and vitamin D handling. PKC activates NFκB, a transcription factor complex controlling FGF23 expression. Here, we analyzed the impact of PKC on FGF23 synthesis. Fgf23 expression was analyzed by qRT-PCR in UMR106 osteoblast-like cells and in IDG-SW3 osteocytes, and FGF23 protein was measured by ELISA. Phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA), a PKC activator, up-regulated FGF23 production. In contrast, PKC inhibitors calphostin C, Gö6976, sotrastaurin and ruboxistaurin suppressed FGF23 formation. NFκB inhibitor withaferin A abolished the stimulatory effect of PMA on Fgf23. PKC is a powerful regulator of FGF23 synthesis, an effect which is at least partly mediated by NFκB.https://doi.org/10.1371/journal.pone.0211309 |
| spellingShingle | Ludmilla Bär Philipp Hase Michael Föller PKC regulates the production of fibroblast growth factor 23 (FGF23). PLoS ONE |
| title | PKC regulates the production of fibroblast growth factor 23 (FGF23). |
| title_full | PKC regulates the production of fibroblast growth factor 23 (FGF23). |
| title_fullStr | PKC regulates the production of fibroblast growth factor 23 (FGF23). |
| title_full_unstemmed | PKC regulates the production of fibroblast growth factor 23 (FGF23). |
| title_short | PKC regulates the production of fibroblast growth factor 23 (FGF23). |
| title_sort | pkc regulates the production of fibroblast growth factor 23 fgf23 |
| url | https://doi.org/10.1371/journal.pone.0211309 |
| work_keys_str_mv | AT ludmillabar pkcregulatestheproductionoffibroblastgrowthfactor23fgf23 AT philipphase pkcregulatestheproductionoffibroblastgrowthfactor23fgf23 AT michaelfoller pkcregulatestheproductionoffibroblastgrowthfactor23fgf23 |