Hsa_circ_0013561 promotes progression of nasopharyngeal carcinoma by activating JAK2/STAT3 signaling pathway
Objective: Nasopharyngeal Carcinoma (NPC) is a malignancy of epithelium of epithelium of the nasopharynx, with the highest incidence of otolaryngeal malignancies. A growing number of studies confirm that Circular RNA (circRNA) plays an important role in tumor development, including Hsa_circ_0013561....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Brazilian Journal of Otorhinolaryngology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1808869423001301 |
| _version_ | 1827373975411359744 |
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| author | Tian Kaisai Zheng Mantang Yuan Tailei Zheng Liying Chen Xiaoping Jin Mingming Zhang Yi |
| author_facet | Tian Kaisai Zheng Mantang Yuan Tailei Zheng Liying Chen Xiaoping Jin Mingming Zhang Yi |
| author_sort | Tian Kaisai |
| collection | DOAJ |
| description | Objective: Nasopharyngeal Carcinoma (NPC) is a malignancy of epithelium of epithelium of the nasopharynx, with the highest incidence of otolaryngeal malignancies. A growing number of studies confirm that Circular RNA (circRNA) plays an important role in tumor development, including Hsa_circ_0013561. This study aims to elucidate the process and mechanism of NPC regulation hsa_circ_0013561. Methods: In this study, circRNA expression nodes and subcellular localization in NPC tissues were analyzed by fluorescence in situ hybridization. The expression of hsa_circ_0013561 in NPC cells was further clarified by RT-qPCR. At the same time, the lentivirus vector interfered by hsa_circ_0013561 was constructed and transfected. The cell proliferation was detected by CCK-8 method, EdU assay and plate cloning assay. The cell cycle and apoptosis were detected by flow cytometry, and the cell migration ability was detected by wound healing assay and Transwell assay. Western blotting examined the expression of apoptosis, Epithelial-Mesenchymal Transition (EMT)-associated proteins, and Janus Kinase/Signal Transductor and Activator of Transcription (JAK/STAT) signaling pathway-related proteins. Results: The results showed that the expression of hsa_circ_0013561 in NPC samples was significantly upregulated and hsa_circ_0013561 localized in the cytoplasm. After down-regulating hsa_circ_0013561 expression, it significantly inhibited the proliferation and metastasis ability of NPC, inhibited EMT progression, and promoted apoptosis. Further studies showed that interference hsa_circ_0013561 significantly inhibited JAK2/STAT3 signaling pathway activation and induced the expression of apoptosis-related proteins. Conclusion: In summary, we found that hsa_circ_0013561 is a pro-tumor circRNA in NPC, which can reduce the activation of JAK2/STAT3 pathway by knocking down hsa_circ_0013561, thereby slowing down the malignant progression of NPC. Oxford Centre for Evidence-Based Medicine 2011 Levels of Evidence: Level 4. |
| first_indexed | 2024-03-08T11:25:44Z |
| format | Article |
| id | doaj.art-6522c4bb4704447fad92794dd414fc30 |
| institution | Directory Open Access Journal |
| issn | 1808-8694 |
| language | English |
| last_indexed | 2024-03-08T11:25:44Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Otorhinolaryngology |
| spelling | doaj.art-6522c4bb4704447fad92794dd414fc302024-01-26T05:32:39ZengElsevierBrazilian Journal of Otorhinolaryngology1808-86942024-01-01901101362Hsa_circ_0013561 promotes progression of nasopharyngeal carcinoma by activating JAK2/STAT3 signaling pathwayTian Kaisai0Zheng Mantang1Yuan Tailei2Zheng Liying3Chen Xiaoping4Jin Mingming5Zhang Yi6Ningxia Medical University, Postgraduate Training Base in Shanghai Gongli Hospital, Shanghai, China; Shanghai Pudong New Area Gongli Hospital, Department of Otorhinolaryngology & Head and Neck Surgery, Shanghai, China; Shanghai University of Medicine and Health Sciences, Shanghai Key Laboratory of Molecular Imaging, Shanghai, ChinaAffiliated Hospital of Hebei University, Baoding, ChinaNingxia Medical University, Postgraduate Training Base in Shanghai Gongli Hospital, Shanghai, China; Shanghai University of Medicine and Health Sciences, Shanghai Key Laboratory of Molecular Imaging, Shanghai, ChinaNingxia Medical University, Postgraduate Training Base in Shanghai Gongli Hospital, Shanghai, China; Shanghai Pudong New Area Gongli Hospital, Department of Otorhinolaryngology & Head and Neck Surgery, Shanghai, China; Shanghai University of Medicine and Health Sciences, Shanghai Key Laboratory of Molecular Imaging, Shanghai, ChinaShanghai Pudong New Area Gongli Hospital, Department of Otorhinolaryngology & Head and Neck Surgery, Shanghai, ChinaShanghai University of Medicine and Health Sciences, Shanghai Key Laboratory of Molecular Imaging, Shanghai, ChinaShanghai Pudong New Area Gongli Hospital, Department of Otorhinolaryngology & Head and Neck Surgery, Shanghai, China; Corresponding author.Objective: Nasopharyngeal Carcinoma (NPC) is a malignancy of epithelium of epithelium of the nasopharynx, with the highest incidence of otolaryngeal malignancies. A growing number of studies confirm that Circular RNA (circRNA) plays an important role in tumor development, including Hsa_circ_0013561. This study aims to elucidate the process and mechanism of NPC regulation hsa_circ_0013561. Methods: In this study, circRNA expression nodes and subcellular localization in NPC tissues were analyzed by fluorescence in situ hybridization. The expression of hsa_circ_0013561 in NPC cells was further clarified by RT-qPCR. At the same time, the lentivirus vector interfered by hsa_circ_0013561 was constructed and transfected. The cell proliferation was detected by CCK-8 method, EdU assay and plate cloning assay. The cell cycle and apoptosis were detected by flow cytometry, and the cell migration ability was detected by wound healing assay and Transwell assay. Western blotting examined the expression of apoptosis, Epithelial-Mesenchymal Transition (EMT)-associated proteins, and Janus Kinase/Signal Transductor and Activator of Transcription (JAK/STAT) signaling pathway-related proteins. Results: The results showed that the expression of hsa_circ_0013561 in NPC samples was significantly upregulated and hsa_circ_0013561 localized in the cytoplasm. After down-regulating hsa_circ_0013561 expression, it significantly inhibited the proliferation and metastasis ability of NPC, inhibited EMT progression, and promoted apoptosis. Further studies showed that interference hsa_circ_0013561 significantly inhibited JAK2/STAT3 signaling pathway activation and induced the expression of apoptosis-related proteins. Conclusion: In summary, we found that hsa_circ_0013561 is a pro-tumor circRNA in NPC, which can reduce the activation of JAK2/STAT3 pathway by knocking down hsa_circ_0013561, thereby slowing down the malignant progression of NPC. Oxford Centre for Evidence-Based Medicine 2011 Levels of Evidence: Level 4.http://www.sciencedirect.com/science/article/pii/S1808869423001301Nasopharyngeal carcinomaHsa_circ_0013561JAK2/STAT3EMT |
| spellingShingle | Tian Kaisai Zheng Mantang Yuan Tailei Zheng Liying Chen Xiaoping Jin Mingming Zhang Yi Hsa_circ_0013561 promotes progression of nasopharyngeal carcinoma by activating JAK2/STAT3 signaling pathway Brazilian Journal of Otorhinolaryngology Nasopharyngeal carcinoma Hsa_circ_0013561 JAK2/STAT3 EMT |
| title | Hsa_circ_0013561 promotes progression of nasopharyngeal carcinoma by activating JAK2/STAT3 signaling pathway |
| title_full | Hsa_circ_0013561 promotes progression of nasopharyngeal carcinoma by activating JAK2/STAT3 signaling pathway |
| title_fullStr | Hsa_circ_0013561 promotes progression of nasopharyngeal carcinoma by activating JAK2/STAT3 signaling pathway |
| title_full_unstemmed | Hsa_circ_0013561 promotes progression of nasopharyngeal carcinoma by activating JAK2/STAT3 signaling pathway |
| title_short | Hsa_circ_0013561 promotes progression of nasopharyngeal carcinoma by activating JAK2/STAT3 signaling pathway |
| title_sort | hsa circ 0013561 promotes progression of nasopharyngeal carcinoma by activating jak2 stat3 signaling pathway |
| topic | Nasopharyngeal carcinoma Hsa_circ_0013561 JAK2/STAT3 EMT |
| url | http://www.sciencedirect.com/science/article/pii/S1808869423001301 |
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