Antibacterial Activity and Cell Viability of Biomimetic Magnesian Calcite Coatings on Biodegradable Mg
Mg is a material of choice for biodegradable implants. The main challenge for using Mg in temporary implants is to provide protective surfaces that mitigate its rapid degradation in biological fluids and also confer sufficient cytocompatibility and bacterial resistance to Mg-coated surfaces. Even th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/2/98 |
| _version_ | 1797620122921205760 |
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| author | Monica Popa Mihai Anastasescu Laura M. Stefan Ana-Maria Prelipcean Jose Calderon Moreno |
| author_facet | Monica Popa Mihai Anastasescu Laura M. Stefan Ana-Maria Prelipcean Jose Calderon Moreno |
| author_sort | Monica Popa |
| collection | DOAJ |
| description | Mg is a material of choice for biodegradable implants. The main challenge for using Mg in temporary implants is to provide protective surfaces that mitigate its rapid degradation in biological fluids and also confer sufficient cytocompatibility and bacterial resistance to Mg-coated surfaces. Even though carbonate mineralization is the most important source of biominerals, such as the skeletons and shells of many marine organisms, there has been little success in the controlled growth of carbonate layers by synthetic processes. We present here the formation mechanism, antibacterial activity, and cell viability of magnesian calcite biomimetic coatings grown on biodegradable Mg via a green, one-step route. Cell compatibility assessment showed cell viability higher than 80% after 72 h using fibroblast cells (NCTC, clone L929) and higher than 60% after 72 h using human osteoblast-like cells (SaOS-2); the cells displayed a normal appearance and a density similar to the control sample. Antimicrobial potential evaluation against both Gram-positive (<i>Staphylococcus aureus</i> (ATCC 25923)) and Gram-negative (<i>Pseudomonas aeruginosa</i> (ATCC 27853)) strains demonstrated that the coated samples significantly inhibited bacterial adhesion and biofilm formation compared to the untreated control. Calcite coatings grown on biodegradable Mg by a single coating process showed the necessary properties of cell compatibility and bacterial resistance for application in surface-modified Mg biomaterials for temporary implants. |
| first_indexed | 2024-03-11T08:37:06Z |
| format | Article |
| id | doaj.art-6522dc28308a435da0161cfa248c92b4 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T08:37:06Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-6522dc28308a435da0161cfa248c92b42023-11-16T21:24:38ZengMDPI AGJournal of Functional Biomaterials2079-49832023-02-011429810.3390/jfb14020098Antibacterial Activity and Cell Viability of Biomimetic Magnesian Calcite Coatings on Biodegradable MgMonica Popa0Mihai Anastasescu1Laura M. Stefan2Ana-Maria Prelipcean3Jose Calderon Moreno4Oxide Compounds and Materials Science Laboratory, “Ilie Murgulescu” Institute of Physical Chemistry, 202 Splaiul Independentei, 060021 Bucharest, RomaniaSurface Chemistry and Catalysis Laboratory, “Ilie Murgulescu” Institute of Physical Chemistry, 202 Splaiul Independentei, 060021 Bucharest, RomaniaDepartment of Cellular and Molecular Biology, National Institute of R&D for Biological Sciences, 296 Splaiul Independentei, 060031 Bucharest, RomaniaDepartment of Cellular and Molecular Biology, National Institute of R&D for Biological Sciences, 296 Splaiul Independentei, 060031 Bucharest, RomaniaSurface Chemistry and Catalysis Laboratory, “Ilie Murgulescu” Institute of Physical Chemistry, 202 Splaiul Independentei, 060021 Bucharest, RomaniaMg is a material of choice for biodegradable implants. The main challenge for using Mg in temporary implants is to provide protective surfaces that mitigate its rapid degradation in biological fluids and also confer sufficient cytocompatibility and bacterial resistance to Mg-coated surfaces. Even though carbonate mineralization is the most important source of biominerals, such as the skeletons and shells of many marine organisms, there has been little success in the controlled growth of carbonate layers by synthetic processes. We present here the formation mechanism, antibacterial activity, and cell viability of magnesian calcite biomimetic coatings grown on biodegradable Mg via a green, one-step route. Cell compatibility assessment showed cell viability higher than 80% after 72 h using fibroblast cells (NCTC, clone L929) and higher than 60% after 72 h using human osteoblast-like cells (SaOS-2); the cells displayed a normal appearance and a density similar to the control sample. Antimicrobial potential evaluation against both Gram-positive (<i>Staphylococcus aureus</i> (ATCC 25923)) and Gram-negative (<i>Pseudomonas aeruginosa</i> (ATCC 27853)) strains demonstrated that the coated samples significantly inhibited bacterial adhesion and biofilm formation compared to the untreated control. Calcite coatings grown on biodegradable Mg by a single coating process showed the necessary properties of cell compatibility and bacterial resistance for application in surface-modified Mg biomaterials for temporary implants.https://www.mdpi.com/2079-4983/14/2/98resorbable biomaterialCaCO<sub>3</sub>amorphous calcium carbonate (ACC)corrosion protective filmantibacterialcell viability |
| spellingShingle | Monica Popa Mihai Anastasescu Laura M. Stefan Ana-Maria Prelipcean Jose Calderon Moreno Antibacterial Activity and Cell Viability of Biomimetic Magnesian Calcite Coatings on Biodegradable Mg Journal of Functional Biomaterials resorbable biomaterial CaCO<sub>3</sub> amorphous calcium carbonate (ACC) corrosion protective film antibacterial cell viability |
| title | Antibacterial Activity and Cell Viability of Biomimetic Magnesian Calcite Coatings on Biodegradable Mg |
| title_full | Antibacterial Activity and Cell Viability of Biomimetic Magnesian Calcite Coatings on Biodegradable Mg |
| title_fullStr | Antibacterial Activity and Cell Viability of Biomimetic Magnesian Calcite Coatings on Biodegradable Mg |
| title_full_unstemmed | Antibacterial Activity and Cell Viability of Biomimetic Magnesian Calcite Coatings on Biodegradable Mg |
| title_short | Antibacterial Activity and Cell Viability of Biomimetic Magnesian Calcite Coatings on Biodegradable Mg |
| title_sort | antibacterial activity and cell viability of biomimetic magnesian calcite coatings on biodegradable mg |
| topic | resorbable biomaterial CaCO<sub>3</sub> amorphous calcium carbonate (ACC) corrosion protective film antibacterial cell viability |
| url | https://www.mdpi.com/2079-4983/14/2/98 |
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