A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145
Prostate cancer accounts for 14% of male cancer-related fatalities in the UK. Given the challenges associated with hormone-based therapies in the context of androgen-independent prostate cancer, there is an imperative need for research into anticancer drugs. N0821, a peptide belonging to the Trp-Arg...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/46/3/144 |
| _version_ | 1827306687909855232 |
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| author | Ye-eun Kim Ki-Young Kim |
| author_facet | Ye-eun Kim Ki-Young Kim |
| author_sort | Ye-eun Kim |
| collection | DOAJ |
| description | Prostate cancer accounts for 14% of male cancer-related fatalities in the UK. Given the challenges associated with hormone-based therapies in the context of androgen-independent prostate cancer, there is an imperative need for research into anticancer drugs. N0821, a peptide belonging to the Trp-Arg dense region and derived from the homologous region of various bee species, shows substantial potential for an anticancer effect. Both MTT assays and 3D spheroid assays were conducted to substantiate its antiproliferation potential and strongly indicated the antiproliferation effect of N0820 (WWWWRWWRKI) and N0821 (YWWWWRWWRKI). Notably, the mechanism underlying this effect is related to the downregulation of CCNA2 and the upregulation of CCNE1. Cell cycle arrest results from the reduction of CCNA2 in the S/G2 phase, leading to the accumulation of CCNE1. Our peptides were predicted to make an α-helix structure. This can act as an ion channel in the cell membrane. Therefore, we analyzed genes implicated in the influx of calcium ions into the mitochondria. Trp-Arg dense-region peptides are known for their antibacterial properties in targeting cell membranes, making the development of resistance less likely. Hence, further research in this area is essential and promising. |
| first_indexed | 2024-04-24T18:25:39Z |
| format | Article |
| id | doaj.art-65240e3f7daf4c11937518214f141419 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-04-24T18:25:39Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-65240e3f7daf4c11937518214f1414192024-03-27T13:31:25ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-03-014632251226210.3390/cimb46030144A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145Ye-eun Kim0Ki-Young Kim1Graduate School of Biotechnology, Kyung Hee University, Seocheon, Giheung, Yongin 17104, Republic of KoreaGraduate School of Biotechnology, Kyung Hee University, Seocheon, Giheung, Yongin 17104, Republic of KoreaProstate cancer accounts for 14% of male cancer-related fatalities in the UK. Given the challenges associated with hormone-based therapies in the context of androgen-independent prostate cancer, there is an imperative need for research into anticancer drugs. N0821, a peptide belonging to the Trp-Arg dense region and derived from the homologous region of various bee species, shows substantial potential for an anticancer effect. Both MTT assays and 3D spheroid assays were conducted to substantiate its antiproliferation potential and strongly indicated the antiproliferation effect of N0820 (WWWWRWWRKI) and N0821 (YWWWWRWWRKI). Notably, the mechanism underlying this effect is related to the downregulation of CCNA2 and the upregulation of CCNE1. Cell cycle arrest results from the reduction of CCNA2 in the S/G2 phase, leading to the accumulation of CCNE1. Our peptides were predicted to make an α-helix structure. This can act as an ion channel in the cell membrane. Therefore, we analyzed genes implicated in the influx of calcium ions into the mitochondria. Trp-Arg dense-region peptides are known for their antibacterial properties in targeting cell membranes, making the development of resistance less likely. Hence, further research in this area is essential and promising.https://www.mdpi.com/1467-3045/46/3/144bee Trp-Arg dense peptideprostate cancerandrogen-independent prostate cancer |
| spellingShingle | Ye-eun Kim Ki-Young Kim A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145 Current Issues in Molecular Biology bee Trp-Arg dense peptide prostate cancer androgen-independent prostate cancer |
| title | A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145 |
| title_full | A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145 |
| title_fullStr | A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145 |
| title_full_unstemmed | A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145 |
| title_short | A Bee Trp-Arg Dense Peptide with Antiproliferation Efficacy against the Prostate Cancer Cell Line DU145 |
| title_sort | bee trp arg dense peptide with antiproliferation efficacy against the prostate cancer cell line du145 |
| topic | bee Trp-Arg dense peptide prostate cancer androgen-independent prostate cancer |
| url | https://www.mdpi.com/1467-3045/46/3/144 |
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