Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological compl...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/3/630 |
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| author | Marcela Vlcnovska Aneta Stossova Michaela Kuchynka Veronika Dillingerova Hana Polanska Michal Masarik Roman Hrstka Vojtech Adam Viktor Kanicky Tomas Vaculovic Marketa Vaculovicova |
| author_facet | Marcela Vlcnovska Aneta Stossova Michaela Kuchynka Veronika Dillingerova Hana Polanska Michal Masarik Roman Hrstka Vojtech Adam Viktor Kanicky Tomas Vaculovic Marketa Vaculovicova |
| author_sort | Marcela Vlcnovska |
| collection | DOAJ |
| description | Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1–14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein. |
| first_indexed | 2024-03-09T03:40:24Z |
| format | Article |
| id | doaj.art-6529ef9eb7ec40a39b4acb196a9e02f9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T03:40:24Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6529ef9eb7ec40a39b4acb196a9e02f92023-12-03T14:41:36ZengMDPI AGMolecules1420-30492021-01-0126363010.3390/molecules26030630Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS DetectionMarcela Vlcnovska0Aneta Stossova1Michaela Kuchynka2Veronika Dillingerova3Hana Polanska4Michal Masarik5Roman Hrstka6Vojtech Adam7Viktor Kanicky8Tomas Vaculovic9Marketa Vaculovicova10Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicDepartment of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, CZ-625 00 Brno, Czech RepublicDepartment of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, CZ-625 00 Brno, Czech RepublicDepartment of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, CZ-625 00 Brno, Czech RepublicDepartment of Pathological Physiology, Faculty of Medicine, Masaryk University/Kamenice 5, CZ-625 00 Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicResearch Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Zluty kopec 7, CZ-656 53 Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicDepartment of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, CZ-625 00 Brno, Czech RepublicDepartment of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, CZ-625 00 Brno, Czech RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech RepublicImmunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1–14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein.https://www.mdpi.com/1420-3049/26/3/630protein p53dot-blotantibody |
| spellingShingle | Marcela Vlcnovska Aneta Stossova Michaela Kuchynka Veronika Dillingerova Hana Polanska Michal Masarik Roman Hrstka Vojtech Adam Viktor Kanicky Tomas Vaculovic Marketa Vaculovicova Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection Molecules protein p53 dot-blot antibody |
| title | Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection |
| title_full | Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection |
| title_fullStr | Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection |
| title_full_unstemmed | Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection |
| title_short | Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection |
| title_sort | comparison of metal nanoparticles au ag eu cd used for immunoanalysis using la icp ms detection |
| topic | protein p53 dot-blot antibody |
| url | https://www.mdpi.com/1420-3049/26/3/630 |
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