Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp.
The agriculturally important genus Colletotrichum is an emerging model pathogen for studying defense in Arabidopsis. During the process of screening for novel pathogenic Colletotrichum isolates on Arabidopsis, we found significant differences in defense responses between detached and attached leaf a...
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Format: | Article |
Language: | English |
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The American Phytopathological Society
2007-10-01
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Series: | Molecular Plant-Microbe Interactions |
Subjects: | |
Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-10-1308 |
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author | Guosheng Liu Regan Kennedy David L. Greenshields Gary Peng Lily Forseille Gopalan Selvaraj Yangdou Wei |
author_facet | Guosheng Liu Regan Kennedy David L. Greenshields Gary Peng Lily Forseille Gopalan Selvaraj Yangdou Wei |
author_sort | Guosheng Liu |
collection | DOAJ |
description | The agriculturally important genus Colletotrichum is an emerging model pathogen for studying defense in Arabidopsis. During the process of screening for novel pathogenic Colletotrichum isolates on Arabidopsis, we found significant differences in defense responses between detached and attached leaf assays. A near-adapted isolate Colletotrichum linicola A1 could launch a typical infection only on detached, but not attached, Arabidopsis leaves. Remarkably, resistance gene-like locus RCH1-mediated resistance in intact plants also was compromised in detached leaves during the attacks with the virulent reference isolate C. higginsianum. The differences in symptom development between the detached leaf and intact plant assays were further confirmed on defense-defective mutants following inoculation with C. higginsianum, where the greatest inconsistency occurred on ethylene-insensitive mutants. In intact Arabidopsis plants, both the salicylic acid- and ethylene-dependent pathways were required for resistance to C. higginsianum and were associated with induced expression of pathogenesis-related genes PR1 and PDF1.2. In contrast, disease symptom development in detached leaves appeared to be uncoupled from these defense pathways and more closely associated with senescence: an observation substantiated by coordinated gene expression analysis and disease symptom development, and chemically and genetically mimicking senescence. |
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institution | Directory Open Access Journal |
issn | 0894-0282 1943-7706 |
language | English |
last_indexed | 2024-04-13T04:26:50Z |
publishDate | 2007-10-01 |
publisher | The American Phytopathological Society |
record_format | Article |
series | Molecular Plant-Microbe Interactions |
spelling | doaj.art-652ca921db324cb7a01c1edbdaf6e4ed2022-12-22T03:02:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-10-0120101308131910.1094/MPMI-20-10-1308Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp.Guosheng LiuRegan KennedyDavid L. GreenshieldsGary PengLily ForseilleGopalan SelvarajYangdou WeiThe agriculturally important genus Colletotrichum is an emerging model pathogen for studying defense in Arabidopsis. During the process of screening for novel pathogenic Colletotrichum isolates on Arabidopsis, we found significant differences in defense responses between detached and attached leaf assays. A near-adapted isolate Colletotrichum linicola A1 could launch a typical infection only on detached, but not attached, Arabidopsis leaves. Remarkably, resistance gene-like locus RCH1-mediated resistance in intact plants also was compromised in detached leaves during the attacks with the virulent reference isolate C. higginsianum. The differences in symptom development between the detached leaf and intact plant assays were further confirmed on defense-defective mutants following inoculation with C. higginsianum, where the greatest inconsistency occurred on ethylene-insensitive mutants. In intact Arabidopsis plants, both the salicylic acid- and ethylene-dependent pathways were required for resistance to C. higginsianum and were associated with induced expression of pathogenesis-related genes PR1 and PDF1.2. In contrast, disease symptom development in detached leaves appeared to be uncoupled from these defense pathways and more closely associated with senescence: an observation substantiated by coordinated gene expression analysis and disease symptom development, and chemically and genetically mimicking senescence.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-10-1308anthracnosebiotrophynecrotrophynonhost resistance |
spellingShingle | Guosheng Liu Regan Kennedy David L. Greenshields Gary Peng Lily Forseille Gopalan Selvaraj Yangdou Wei Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp. Molecular Plant-Microbe Interactions anthracnose biotrophy necrotrophy nonhost resistance |
title | Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp. |
title_full | Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp. |
title_fullStr | Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp. |
title_full_unstemmed | Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp. |
title_short | Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp. |
title_sort | detached and attached arabidopsis leaf assays reveal distinctive defense responses against hemibiotrophic colletotrichum spp |
topic | anthracnose biotrophy necrotrophy nonhost resistance |
url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-10-1308 |
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