Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp.

The agriculturally important genus Colletotrichum is an emerging model pathogen for studying defense in Arabidopsis. During the process of screening for novel pathogenic Colletotrichum isolates on Arabidopsis, we found significant differences in defense responses between detached and attached leaf a...

Full description

Bibliographic Details
Main Authors: Guosheng Liu, Regan Kennedy, David L. Greenshields, Gary Peng, Lily Forseille, Gopalan Selvaraj, Yangdou Wei
Format: Article
Language:English
Published: The American Phytopathological Society 2007-10-01
Series:Molecular Plant-Microbe Interactions
Subjects:
Online Access:https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-10-1308
_version_ 1811291264247660544
author Guosheng Liu
Regan Kennedy
David L. Greenshields
Gary Peng
Lily Forseille
Gopalan Selvaraj
Yangdou Wei
author_facet Guosheng Liu
Regan Kennedy
David L. Greenshields
Gary Peng
Lily Forseille
Gopalan Selvaraj
Yangdou Wei
author_sort Guosheng Liu
collection DOAJ
description The agriculturally important genus Colletotrichum is an emerging model pathogen for studying defense in Arabidopsis. During the process of screening for novel pathogenic Colletotrichum isolates on Arabidopsis, we found significant differences in defense responses between detached and attached leaf assays. A near-adapted isolate Colletotrichum linicola A1 could launch a typical infection only on detached, but not attached, Arabidopsis leaves. Remarkably, resistance gene-like locus RCH1-mediated resistance in intact plants also was compromised in detached leaves during the attacks with the virulent reference isolate C. higginsianum. The differences in symptom development between the detached leaf and intact plant assays were further confirmed on defense-defective mutants following inoculation with C. higginsianum, where the greatest inconsistency occurred on ethylene-insensitive mutants. In intact Arabidopsis plants, both the salicylic acid- and ethylene-dependent pathways were required for resistance to C. higginsianum and were associated with induced expression of pathogenesis-related genes PR1 and PDF1.2. In contrast, disease symptom development in detached leaves appeared to be uncoupled from these defense pathways and more closely associated with senescence: an observation substantiated by coordinated gene expression analysis and disease symptom development, and chemically and genetically mimicking senescence.
first_indexed 2024-04-13T04:26:50Z
format Article
id doaj.art-652ca921db324cb7a01c1edbdaf6e4ed
institution Directory Open Access Journal
issn 0894-0282
1943-7706
language English
last_indexed 2024-04-13T04:26:50Z
publishDate 2007-10-01
publisher The American Phytopathological Society
record_format Article
series Molecular Plant-Microbe Interactions
spelling doaj.art-652ca921db324cb7a01c1edbdaf6e4ed2022-12-22T03:02:28ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-10-0120101308131910.1094/MPMI-20-10-1308Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp.Guosheng LiuRegan KennedyDavid L. GreenshieldsGary PengLily ForseilleGopalan SelvarajYangdou WeiThe agriculturally important genus Colletotrichum is an emerging model pathogen for studying defense in Arabidopsis. During the process of screening for novel pathogenic Colletotrichum isolates on Arabidopsis, we found significant differences in defense responses between detached and attached leaf assays. A near-adapted isolate Colletotrichum linicola A1 could launch a typical infection only on detached, but not attached, Arabidopsis leaves. Remarkably, resistance gene-like locus RCH1-mediated resistance in intact plants also was compromised in detached leaves during the attacks with the virulent reference isolate C. higginsianum. The differences in symptom development between the detached leaf and intact plant assays were further confirmed on defense-defective mutants following inoculation with C. higginsianum, where the greatest inconsistency occurred on ethylene-insensitive mutants. In intact Arabidopsis plants, both the salicylic acid- and ethylene-dependent pathways were required for resistance to C. higginsianum and were associated with induced expression of pathogenesis-related genes PR1 and PDF1.2. In contrast, disease symptom development in detached leaves appeared to be uncoupled from these defense pathways and more closely associated with senescence: an observation substantiated by coordinated gene expression analysis and disease symptom development, and chemically and genetically mimicking senescence.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-10-1308anthracnosebiotrophynecrotrophynonhost resistance
spellingShingle Guosheng Liu
Regan Kennedy
David L. Greenshields
Gary Peng
Lily Forseille
Gopalan Selvaraj
Yangdou Wei
Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp.
Molecular Plant-Microbe Interactions
anthracnose
biotrophy
necrotrophy
nonhost resistance
title Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp.
title_full Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp.
title_fullStr Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp.
title_full_unstemmed Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp.
title_short Detached and Attached Arabidopsis Leaf Assays Reveal Distinctive Defense Responses Against Hemibiotrophic Colletotrichum spp.
title_sort detached and attached arabidopsis leaf assays reveal distinctive defense responses against hemibiotrophic colletotrichum spp
topic anthracnose
biotrophy
necrotrophy
nonhost resistance
url https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-10-1308
work_keys_str_mv AT guoshengliu detachedandattachedarabidopsisleafassaysrevealdistinctivedefenseresponsesagainsthemibiotrophiccolletotrichumspp
AT regankennedy detachedandattachedarabidopsisleafassaysrevealdistinctivedefenseresponsesagainsthemibiotrophiccolletotrichumspp
AT davidlgreenshields detachedandattachedarabidopsisleafassaysrevealdistinctivedefenseresponsesagainsthemibiotrophiccolletotrichumspp
AT garypeng detachedandattachedarabidopsisleafassaysrevealdistinctivedefenseresponsesagainsthemibiotrophiccolletotrichumspp
AT lilyforseille detachedandattachedarabidopsisleafassaysrevealdistinctivedefenseresponsesagainsthemibiotrophiccolletotrichumspp
AT gopalanselvaraj detachedandattachedarabidopsisleafassaysrevealdistinctivedefenseresponsesagainsthemibiotrophiccolletotrichumspp
AT yangdouwei detachedandattachedarabidopsisleafassaysrevealdistinctivedefenseresponsesagainsthemibiotrophiccolletotrichumspp