Characterization of the TnsD-<it>attTn7 </it>complex that promotes site-specific insertion of <it>Tn7</it>
<p>Abstract</p> <p>The bacterial transposon <it>Tn7 </it>is distinguished by its ability to recognize a specific site called <it>attTn7</it>, and insert just downstream of the highly conserved chromosomal <it>glmS </it>gene. TnsD is one of four t...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-07-01
|
| Series: | Mobile DNA |
| Online Access: | http://www.mobilednajournal.com/content/1/1/18 |
| _version_ | 1828367127128571904 |
|---|---|
| author | Mitra Rupak McKenzie Gregory J Yi Liang Lee Cherline A Craig Nancy L |
| author_facet | Mitra Rupak McKenzie Gregory J Yi Liang Lee Cherline A Craig Nancy L |
| author_sort | Mitra Rupak |
| collection | DOAJ |
| description | <p>Abstract</p> <p>The bacterial transposon <it>Tn7 </it>is distinguished by its ability to recognize a specific site called <it>attTn7</it>, and insert just downstream of the highly conserved chromosomal <it>glmS </it>gene. TnsD is one of four transposon-encoded polypeptides (TnsABC+D) required for site-specific insertion of Tn<it>7 </it>into <it>attTn7</it>, and is the target site-selector that binds to a highly conserved sequence in the end of the <it>glmS </it>protein coding region. In this study, we identified important nucleotides within this region that are crucial for TnsD-<it>attTn7 </it>interaction. We also probed the regions of TnsD that interact with <it>attTn7 </it>and found that there are important DNA-binding determinants throughout the entire length of the protein, including an amino-terminal CCCH zinc-finger motif. A key role of TnsD is to recruit the non-sequence specific DNA-binding protein TnsC to <it>attTn7</it>; TnsC also interacts with and controls both the TnsA and TnsB subunits of the Tn7 transposase. TnsC stimulates the binding of TnsD to <it>attTn7 in vivo</it>, and TnsCD and TnsD can also interact in the absence of DNA and localize their interaction domains to the N-terminal region of each protein.</p> |
| first_indexed | 2024-04-14T05:54:05Z |
| format | Article |
| id | doaj.art-652d2bb1ee364466b0520a8036a3427b |
| institution | Directory Open Access Journal |
| issn | 1759-8753 |
| language | English |
| last_indexed | 2024-04-14T05:54:05Z |
| publishDate | 2010-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Mobile DNA |
| spelling | doaj.art-652d2bb1ee364466b0520a8036a3427b2022-12-22T02:08:59ZengBMCMobile DNA1759-87532010-07-01111810.1186/1759-8753-1-18Characterization of the TnsD-<it>attTn7 </it>complex that promotes site-specific insertion of <it>Tn7</it>Mitra RupakMcKenzie Gregory JYi LiangLee Cherline ACraig Nancy L<p>Abstract</p> <p>The bacterial transposon <it>Tn7 </it>is distinguished by its ability to recognize a specific site called <it>attTn7</it>, and insert just downstream of the highly conserved chromosomal <it>glmS </it>gene. TnsD is one of four transposon-encoded polypeptides (TnsABC+D) required for site-specific insertion of Tn<it>7 </it>into <it>attTn7</it>, and is the target site-selector that binds to a highly conserved sequence in the end of the <it>glmS </it>protein coding region. In this study, we identified important nucleotides within this region that are crucial for TnsD-<it>attTn7 </it>interaction. We also probed the regions of TnsD that interact with <it>attTn7 </it>and found that there are important DNA-binding determinants throughout the entire length of the protein, including an amino-terminal CCCH zinc-finger motif. A key role of TnsD is to recruit the non-sequence specific DNA-binding protein TnsC to <it>attTn7</it>; TnsC also interacts with and controls both the TnsA and TnsB subunits of the Tn7 transposase. TnsC stimulates the binding of TnsD to <it>attTn7 in vivo</it>, and TnsCD and TnsD can also interact in the absence of DNA and localize their interaction domains to the N-terminal region of each protein.</p>http://www.mobilednajournal.com/content/1/1/18 |
| spellingShingle | Mitra Rupak McKenzie Gregory J Yi Liang Lee Cherline A Craig Nancy L Characterization of the TnsD-<it>attTn7 </it>complex that promotes site-specific insertion of <it>Tn7</it> Mobile DNA |
| title | Characterization of the TnsD-<it>attTn7 </it>complex that promotes site-specific insertion of <it>Tn7</it> |
| title_full | Characterization of the TnsD-<it>attTn7 </it>complex that promotes site-specific insertion of <it>Tn7</it> |
| title_fullStr | Characterization of the TnsD-<it>attTn7 </it>complex that promotes site-specific insertion of <it>Tn7</it> |
| title_full_unstemmed | Characterization of the TnsD-<it>attTn7 </it>complex that promotes site-specific insertion of <it>Tn7</it> |
| title_short | Characterization of the TnsD-<it>attTn7 </it>complex that promotes site-specific insertion of <it>Tn7</it> |
| title_sort | characterization of the tnsd it atttn7 it complex that promotes site specific insertion of it tn7 it |
| url | http://www.mobilednajournal.com/content/1/1/18 |
| work_keys_str_mv | AT mitrarupak characterizationofthetnsditatttn7itcomplexthatpromotessitespecificinsertionofittn7it AT mckenziegregoryj characterizationofthetnsditatttn7itcomplexthatpromotessitespecificinsertionofittn7it AT yiliang characterizationofthetnsditatttn7itcomplexthatpromotessitespecificinsertionofittn7it AT leecherlinea characterizationofthetnsditatttn7itcomplexthatpromotessitespecificinsertionofittn7it AT craignancyl characterizationofthetnsditatttn7itcomplexthatpromotessitespecificinsertionofittn7it |