Genetic Analysis of the Hsm3 Protein Function in Yeast <i>Saccharomyces cerevisiae</i> NuB4 Complex
In the nuclear compartment of yeast, NuB4 core complex consists of three proteins, Hat1, Hat2, and Hif1, and interacts with a number of other factors. In particular, it was shown that NuB4 complex physically interacts with Hsm3p. Early we demonstrated that the gene <i>HSM3</i> participat...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/12/7/1083 |
| _version_ | 1797527100545040384 |
|---|---|
| author | Tatiyana A. Evstyukhina Elena A. Alekseeva Dmitriy V. Fedorov Vyacheslav T. Peshekhonov Vladimir G. Korolev |
| author_facet | Tatiyana A. Evstyukhina Elena A. Alekseeva Dmitriy V. Fedorov Vyacheslav T. Peshekhonov Vladimir G. Korolev |
| author_sort | Tatiyana A. Evstyukhina |
| collection | DOAJ |
| description | In the nuclear compartment of yeast, NuB4 core complex consists of three proteins, Hat1, Hat2, and Hif1, and interacts with a number of other factors. In particular, it was shown that NuB4 complex physically interacts with Hsm3p. Early we demonstrated that the gene <i>HSM3</i> participates in the control of replicative and reparative spontaneous mutagenesis, and that <i>hsm3Δ</i> mutants increase the frequency of mutations induced by different mutagens. It was previously believed that the <i>HSM3</i> gene controlled only some minor repair processes in the cell, but later it was suggested that it had a chaperone function with its participation in proteasome assembly. In this work, we analyzed the properties of three <i>hsm3Δ</i>, <i>hif1Δ</i>, and <i>hat1Δ</i> mutants. The results obtained showed that the Hsm3 protein may be a functional subunit of NuB4 complex. It has been shown that <i>hsm3</i>- and <i>hif1</i>-dependent UV-induced mutagenesis is completely suppressed by inactivation of the Polη polymerase. We showed a significant role of Polη for <i>hsm3</i>-dependent mutagenesis at non-bipyrimidine sites (NBP sites). The efficiency of expression of <i>RNR</i> (RiboNucleotid Reducase) genes after UV irradiation in <i>hsm3Δ</i> and <i>hif1Δ</i> mutants was several times lower than in wild-type cells. Thus, we have presented evidence that significant increase in the dNTP levels suppress <i>hsm3</i>- and <i>hif1</i>-dependent mutagenesis and Polη is responsible for <i>hsm3</i>- and <i>hif1</i>-dependent mutagenesis. |
| first_indexed | 2024-03-10T09:38:12Z |
| format | Article |
| id | doaj.art-652ee8d70d824c0b847aa5c92d9ef766 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T09:38:12Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-652ee8d70d824c0b847aa5c92d9ef7662023-11-22T03:51:21ZengMDPI AGGenes2073-44252021-07-01127108310.3390/genes12071083Genetic Analysis of the Hsm3 Protein Function in Yeast <i>Saccharomyces cerevisiae</i> NuB4 ComplexTatiyana A. Evstyukhina0Elena A. Alekseeva1Dmitriy V. Fedorov2Vyacheslav T. Peshekhonov3Vladimir G. Korolev4Laboratory of Eukaryotic Genetics, Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaLaboratory of Eukaryotic Genetics, Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaLaboratory of Eukaryotic Genetics, Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaLaboratory of Eukaryotic Genetics, Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaLaboratory of Eukaryotic Genetics, Department of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaIn the nuclear compartment of yeast, NuB4 core complex consists of three proteins, Hat1, Hat2, and Hif1, and interacts with a number of other factors. In particular, it was shown that NuB4 complex physically interacts with Hsm3p. Early we demonstrated that the gene <i>HSM3</i> participates in the control of replicative and reparative spontaneous mutagenesis, and that <i>hsm3Δ</i> mutants increase the frequency of mutations induced by different mutagens. It was previously believed that the <i>HSM3</i> gene controlled only some minor repair processes in the cell, but later it was suggested that it had a chaperone function with its participation in proteasome assembly. In this work, we analyzed the properties of three <i>hsm3Δ</i>, <i>hif1Δ</i>, and <i>hat1Δ</i> mutants. The results obtained showed that the Hsm3 protein may be a functional subunit of NuB4 complex. It has been shown that <i>hsm3</i>- and <i>hif1</i>-dependent UV-induced mutagenesis is completely suppressed by inactivation of the Polη polymerase. We showed a significant role of Polη for <i>hsm3</i>-dependent mutagenesis at non-bipyrimidine sites (NBP sites). The efficiency of expression of <i>RNR</i> (RiboNucleotid Reducase) genes after UV irradiation in <i>hsm3Δ</i> and <i>hif1Δ</i> mutants was several times lower than in wild-type cells. Thus, we have presented evidence that significant increase in the dNTP levels suppress <i>hsm3</i>- and <i>hif1</i>-dependent mutagenesis and Polη is responsible for <i>hsm3</i>- and <i>hif1</i>-dependent mutagenesis.https://www.mdpi.com/2073-4425/12/7/1083yeast<i>Saccharomyces cerevisiae</i>NuB4 complex<i>HSM3</i> gene<i>HIF1</i> geneUV-induced mutagenesis |
| spellingShingle | Tatiyana A. Evstyukhina Elena A. Alekseeva Dmitriy V. Fedorov Vyacheslav T. Peshekhonov Vladimir G. Korolev Genetic Analysis of the Hsm3 Protein Function in Yeast <i>Saccharomyces cerevisiae</i> NuB4 Complex Genes yeast <i>Saccharomyces cerevisiae</i> NuB4 complex <i>HSM3</i> gene <i>HIF1</i> gene UV-induced mutagenesis |
| title | Genetic Analysis of the Hsm3 Protein Function in Yeast <i>Saccharomyces cerevisiae</i> NuB4 Complex |
| title_full | Genetic Analysis of the Hsm3 Protein Function in Yeast <i>Saccharomyces cerevisiae</i> NuB4 Complex |
| title_fullStr | Genetic Analysis of the Hsm3 Protein Function in Yeast <i>Saccharomyces cerevisiae</i> NuB4 Complex |
| title_full_unstemmed | Genetic Analysis of the Hsm3 Protein Function in Yeast <i>Saccharomyces cerevisiae</i> NuB4 Complex |
| title_short | Genetic Analysis of the Hsm3 Protein Function in Yeast <i>Saccharomyces cerevisiae</i> NuB4 Complex |
| title_sort | genetic analysis of the hsm3 protein function in yeast i saccharomyces cerevisiae i nub4 complex |
| topic | yeast <i>Saccharomyces cerevisiae</i> NuB4 complex <i>HSM3</i> gene <i>HIF1</i> gene UV-induced mutagenesis |
| url | https://www.mdpi.com/2073-4425/12/7/1083 |
| work_keys_str_mv | AT tatiyanaaevstyukhina geneticanalysisofthehsm3proteinfunctioninyeastisaccharomycescerevisiaeinub4complex AT elenaaalekseeva geneticanalysisofthehsm3proteinfunctioninyeastisaccharomycescerevisiaeinub4complex AT dmitriyvfedorov geneticanalysisofthehsm3proteinfunctioninyeastisaccharomycescerevisiaeinub4complex AT vyacheslavtpeshekhonov geneticanalysisofthehsm3proteinfunctioninyeastisaccharomycescerevisiaeinub4complex AT vladimirgkorolev geneticanalysisofthehsm3proteinfunctioninyeastisaccharomycescerevisiaeinub4complex |