Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject
During haemodialysis (HD), platelets (PLTs) are activated and release granule contents. As HD treatment occurs three times a week, it has been demonstrated that PLTs are exhausted due to the repetitive character of the treatment. To identify PLT depletion morphologically, PLT evaluation was perform...
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-10-01
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| Series: | Hematology Reports |
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| Online Access: | http://www.pagepress.org/journals/index.php/hr/article/view/3110 |
| _version_ | 1828046986774839296 |
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| author | Marianne Schoorl Piet C.M. Bartels Mareille Gritters Donna Fluitsma René Musters Menso J. Nubé |
| author_facet | Marianne Schoorl Piet C.M. Bartels Mareille Gritters Donna Fluitsma René Musters Menso J. Nubé |
| author_sort | Marianne Schoorl |
| collection | DOAJ |
| description | During haemodialysis (HD), platelets (PLTs) are activated and release granule contents. As HD treatment occurs three times a week, it has been demonstrated that PLTs are exhausted due to the repetitive character of the treatment. To identify PLT depletion morphologically, PLT evaluation was performed by light microscopy and electron microscopy (EM) in a chronic HD subject and a healthy reference subject. Blood samples were taken before the start of HD treatment for measurement of PLT count, PLT volume and size parameters. Blood smears were screened by light microscopy for qualitative evaluation of PLT granule containing cytoplasm, as indicated by its staining density. Morphological PLT parameters of surface area and size of dense bodies were assessed by EM. Data were compared with results of a group of 20 chronic HD subjects and a group of 20 healthy reference subjects. With respect to the percentage of PLTs with appropriate staining density (> 75%), light microscopic evaluation showed that this value (9%) was within the range of a group of chronic HD subjects, but considerably below the reference range (70%). EM evaluation revealed an average PLT surface area and dense bodies area of respectively 42% and 31%, if the healthy reference subject was set on 100%. PLTs from a chronic HD subject are considerably smaller and substantially less granular than PLTs from a healthy reference subject. These findings support the hypothesis of PLT depletion in chronic HD subjects due to frequent PLT activation and/or increased urea concentrations. |
| first_indexed | 2024-04-10T18:34:09Z |
| format | Article |
| id | doaj.art-652fb18d6fac4f3ab999af99e0431850 |
| institution | Directory Open Access Journal |
| issn | 2038-8322 2038-8330 |
| language | English |
| last_indexed | 2024-04-10T18:34:09Z |
| publishDate | 2011-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Hematology Reports |
| spelling | doaj.art-652fb18d6fac4f3ab999af99e04318502023-02-02T02:09:12ZengMDPI AGHematology Reports2038-83222038-83302011-10-0132e15e1510.4081/hr.2011.e151724Electron microscopic observation in case of platelet activation in a chronic haemodialysis subjectMarianne Schoorl0Piet C.M. Bartels1Mareille Gritters2Donna Fluitsma3René Musters4Menso J. Nubé5Department of Clinical Chemistry, Haematology and Immunology, Medical Center Alkmaar, AlkmaarDepartment of Clinical Chemistry, Haematology and Immunology, Medical Center Alkmaar, AlkmaarDepartment of Nephrology, VU Meedical Centre, AmsterdamMolecular Cellbiology and Immunology, VU Meedical Centre, AmsterdamPhysiology, VU Meedical Centre, AmsterdamDepartment of Nephrology, VU Meedical Centre, AmsterdamDuring haemodialysis (HD), platelets (PLTs) are activated and release granule contents. As HD treatment occurs three times a week, it has been demonstrated that PLTs are exhausted due to the repetitive character of the treatment. To identify PLT depletion morphologically, PLT evaluation was performed by light microscopy and electron microscopy (EM) in a chronic HD subject and a healthy reference subject. Blood samples were taken before the start of HD treatment for measurement of PLT count, PLT volume and size parameters. Blood smears were screened by light microscopy for qualitative evaluation of PLT granule containing cytoplasm, as indicated by its staining density. Morphological PLT parameters of surface area and size of dense bodies were assessed by EM. Data were compared with results of a group of 20 chronic HD subjects and a group of 20 healthy reference subjects. With respect to the percentage of PLTs with appropriate staining density (> 75%), light microscopic evaluation showed that this value (9%) was within the range of a group of chronic HD subjects, but considerably below the reference range (70%). EM evaluation revealed an average PLT surface area and dense bodies area of respectively 42% and 31%, if the healthy reference subject was set on 100%. PLTs from a chronic HD subject are considerably smaller and substantially less granular than PLTs from a healthy reference subject. These findings support the hypothesis of PLT depletion in chronic HD subjects due to frequent PLT activation and/or increased urea concentrations.http://www.pagepress.org/journals/index.php/hr/article/view/3110electron microscopy, platelet activation, platelet degranulation, haemodialysis |
| spellingShingle | Marianne Schoorl Piet C.M. Bartels Mareille Gritters Donna Fluitsma René Musters Menso J. Nubé Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject Hematology Reports electron microscopy, platelet activation, platelet degranulation, haemodialysis |
| title | Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject |
| title_full | Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject |
| title_fullStr | Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject |
| title_full_unstemmed | Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject |
| title_short | Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject |
| title_sort | electron microscopic observation in case of platelet activation in a chronic haemodialysis subject |
| topic | electron microscopy, platelet activation, platelet degranulation, haemodialysis |
| url | http://www.pagepress.org/journals/index.php/hr/article/view/3110 |
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