Meet me on the other side: trans-bilayer modulation of a model voltage-gated ion channel activity by membrane electrostatics asymmetry.
While it is accepted that biomembrane asymmetry is generated by proteins and phospholipids distribution, little is known about how electric changes manifested in a monolayer influence functional properties of proteins localized on the opposite leaflet. Herein we used single-molecule electrophysiolog...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3181326?pdf=render |
| _version_ | 1819022062818164736 |
|---|---|
| author | Loredana Mereuta Alina Asandei Tudor Luchian |
| author_facet | Loredana Mereuta Alina Asandei Tudor Luchian |
| author_sort | Loredana Mereuta |
| collection | DOAJ |
| description | While it is accepted that biomembrane asymmetry is generated by proteins and phospholipids distribution, little is known about how electric changes manifested in a monolayer influence functional properties of proteins localized on the opposite leaflet. Herein we used single-molecule electrophysiology and investigated how asymmetric changes in the electrostatics of an artificial lipid membrane monolayer, generated oppositely from where alamethicin--a model voltage-gated ion channel--was added, altered peptide activity. We found that phlorizin, a membrane dipole potential lowering amphiphile, augmented alamethicin activity and transport features, whereas the opposite occurred with RH-421, which enhances the monolayer dipole potential. Further, the monolayer surface potential was decreased via adsorption of sodium dodecyl sulfate, and demonstrated that vectorial modification of it also affected the alamethicin activity in a predictive manner. A new paradigm is suggested according to which asymmetric changes in the monolayer dipole and surface potential extend their effects spatially by altering the intramembrane potential, whose gradient is sensed by distantly located peptides. |
| first_indexed | 2024-12-21T04:17:02Z |
| format | Article |
| id | doaj.art-653189f5f6e34d2f85fd5313973e5912 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T04:17:02Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-653189f5f6e34d2f85fd5313973e59122022-12-21T19:16:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2527610.1371/journal.pone.0025276Meet me on the other side: trans-bilayer modulation of a model voltage-gated ion channel activity by membrane electrostatics asymmetry.Loredana MereutaAlina AsandeiTudor LuchianWhile it is accepted that biomembrane asymmetry is generated by proteins and phospholipids distribution, little is known about how electric changes manifested in a monolayer influence functional properties of proteins localized on the opposite leaflet. Herein we used single-molecule electrophysiology and investigated how asymmetric changes in the electrostatics of an artificial lipid membrane monolayer, generated oppositely from where alamethicin--a model voltage-gated ion channel--was added, altered peptide activity. We found that phlorizin, a membrane dipole potential lowering amphiphile, augmented alamethicin activity and transport features, whereas the opposite occurred with RH-421, which enhances the monolayer dipole potential. Further, the monolayer surface potential was decreased via adsorption of sodium dodecyl sulfate, and demonstrated that vectorial modification of it also affected the alamethicin activity in a predictive manner. A new paradigm is suggested according to which asymmetric changes in the monolayer dipole and surface potential extend their effects spatially by altering the intramembrane potential, whose gradient is sensed by distantly located peptides.http://europepmc.org/articles/PMC3181326?pdf=render |
| spellingShingle | Loredana Mereuta Alina Asandei Tudor Luchian Meet me on the other side: trans-bilayer modulation of a model voltage-gated ion channel activity by membrane electrostatics asymmetry. PLoS ONE |
| title | Meet me on the other side: trans-bilayer modulation of a model voltage-gated ion channel activity by membrane electrostatics asymmetry. |
| title_full | Meet me on the other side: trans-bilayer modulation of a model voltage-gated ion channel activity by membrane electrostatics asymmetry. |
| title_fullStr | Meet me on the other side: trans-bilayer modulation of a model voltage-gated ion channel activity by membrane electrostatics asymmetry. |
| title_full_unstemmed | Meet me on the other side: trans-bilayer modulation of a model voltage-gated ion channel activity by membrane electrostatics asymmetry. |
| title_short | Meet me on the other side: trans-bilayer modulation of a model voltage-gated ion channel activity by membrane electrostatics asymmetry. |
| title_sort | meet me on the other side trans bilayer modulation of a model voltage gated ion channel activity by membrane electrostatics asymmetry |
| url | http://europepmc.org/articles/PMC3181326?pdf=render |
| work_keys_str_mv | AT loredanamereuta meetmeontheothersidetransbilayermodulationofamodelvoltagegatedionchannelactivitybymembraneelectrostaticsasymmetry AT alinaasandei meetmeontheothersidetransbilayermodulationofamodelvoltagegatedionchannelactivitybymembraneelectrostaticsasymmetry AT tudorluchian meetmeontheothersidetransbilayermodulationofamodelvoltagegatedionchannelactivitybymembraneelectrostaticsasymmetry |