Highly Sensitive Luminescent Bioassay Using Recombinant <i>Escherichia coli</i> Biosensor for Rapid Detection of Low Cr(VI) Concentration in Environmental Water
In this study, we constructed a recombinant <i>Escherichia coli</i> strain with different promoters inserted between the chromate-sensing regulator <i>chrB</i> and the reporter gene <i>luxAB</i> to sense low hexavalent chromium (Cr(VI)) concentrations (<0.05 mg...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/10/357 |
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| author | Guey-Horng Wang Chiu-Yu Cheng Teh-Hua Tsai Pin-Kuan Chiang Ying-Chien Chung |
| author_facet | Guey-Horng Wang Chiu-Yu Cheng Teh-Hua Tsai Pin-Kuan Chiang Ying-Chien Chung |
| author_sort | Guey-Horng Wang |
| collection | DOAJ |
| description | In this study, we constructed a recombinant <i>Escherichia coli</i> strain with different promoters inserted between the chromate-sensing regulator <i>chrB</i> and the reporter gene <i>luxAB</i> to sense low hexavalent chromium (Cr(VI)) concentrations (<0.05 mg/L); subsequently, its biosensor characteristics (sensitivity, selectivity, and specificity) for measuring Cr(VI) in various water bodies were evaluated. The luminescence intensity of each biosensor depended on pH, temperature, detection time, coexisting carbon source, coexisting ion, Cr(VI) oxyanion form, Cr(VI) concentration, cell type, and type of medium. Recombinant <i>lux</i>-expressing <i>E. coli</i> with the T7 promoter (T7-lux-<i>E. coli</i>, limit of detection (LOD) = 0.0005 mg/L) had the highest luminescence intensity or was the most sensitive for Cr(VI) detection, followed by <i>E. coli</i> with the T3 promoter (T3-lu<i>x-E. coli</i>, LOD = 0.001 mg/L) and that with the SP6 promoter (SP6-lux-<i>E. coli</i>, LOD = 0.005 mg/L). All biosensors could be used to determine whether the Cr(VI) standard was met in terms of water quality, even when using thawing frozen cells as biosensors after 90-day cryogenic storage. The SP6-lux-<i>E. coli</i> biosensor had the shortest detection time (0.5 h) and the highest adaptability to environmental interference. The T7-lux-<i>E. coli</i> biosensor—with the optimal LOD, a wide measurement range (0.0005–0.5 mg/L), and low deviation (−5.0–7.9%) in detecting Cr(VI) from industrial effluents, domestic effluents, and surface water—is an efficient Cr(VI) biosensor. This unprecedented study is to evaluate recombinant lux <i>E. coli</i> with dissimilar promoters for their possible practice in Cr(VI) measurement in water bodies, and the biosensor performance is clearly superior to that of past systems in terms of detection time, LOD, and detection deviation for real water samples. |
| first_indexed | 2024-03-10T06:41:24Z |
| format | Article |
| id | doaj.art-653534669f8d49c0a28cee92322d544b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T06:41:24Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-653534669f8d49c0a28cee92322d544b2023-11-22T17:35:29ZengMDPI AGBiosensors2079-63742021-09-01111035710.3390/bios11100357Highly Sensitive Luminescent Bioassay Using Recombinant <i>Escherichia coli</i> Biosensor for Rapid Detection of Low Cr(VI) Concentration in Environmental WaterGuey-Horng Wang0Chiu-Yu Cheng1Teh-Hua Tsai2Pin-Kuan Chiang3Ying-Chien Chung4Research Center of Natural Cosmeceuticals Engineering, Xiamen Medical College, Xiamen 361008, ChinaDepartment of Biological Science and Technology, China University of Science and Technology, Taipei 115, TaiwanDepartment of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 10608, TaiwanDepartment of Biological Science and Technology, China University of Science and Technology, Taipei 115, TaiwanDepartment of Biological Science and Technology, China University of Science and Technology, Taipei 115, TaiwanIn this study, we constructed a recombinant <i>Escherichia coli</i> strain with different promoters inserted between the chromate-sensing regulator <i>chrB</i> and the reporter gene <i>luxAB</i> to sense low hexavalent chromium (Cr(VI)) concentrations (<0.05 mg/L); subsequently, its biosensor characteristics (sensitivity, selectivity, and specificity) for measuring Cr(VI) in various water bodies were evaluated. The luminescence intensity of each biosensor depended on pH, temperature, detection time, coexisting carbon source, coexisting ion, Cr(VI) oxyanion form, Cr(VI) concentration, cell type, and type of medium. Recombinant <i>lux</i>-expressing <i>E. coli</i> with the T7 promoter (T7-lux-<i>E. coli</i>, limit of detection (LOD) = 0.0005 mg/L) had the highest luminescence intensity or was the most sensitive for Cr(VI) detection, followed by <i>E. coli</i> with the T3 promoter (T3-lu<i>x-E. coli</i>, LOD = 0.001 mg/L) and that with the SP6 promoter (SP6-lux-<i>E. coli</i>, LOD = 0.005 mg/L). All biosensors could be used to determine whether the Cr(VI) standard was met in terms of water quality, even when using thawing frozen cells as biosensors after 90-day cryogenic storage. The SP6-lux-<i>E. coli</i> biosensor had the shortest detection time (0.5 h) and the highest adaptability to environmental interference. The T7-lux-<i>E. coli</i> biosensor—with the optimal LOD, a wide measurement range (0.0005–0.5 mg/L), and low deviation (−5.0–7.9%) in detecting Cr(VI) from industrial effluents, domestic effluents, and surface water—is an efficient Cr(VI) biosensor. This unprecedented study is to evaluate recombinant lux <i>E. coli</i> with dissimilar promoters for their possible practice in Cr(VI) measurement in water bodies, and the biosensor performance is clearly superior to that of past systems in terms of detection time, LOD, and detection deviation for real water samples.https://www.mdpi.com/2079-6374/11/10/357biosensorhexavalent chromiumlimit of detectionreporter gene |
| spellingShingle | Guey-Horng Wang Chiu-Yu Cheng Teh-Hua Tsai Pin-Kuan Chiang Ying-Chien Chung Highly Sensitive Luminescent Bioassay Using Recombinant <i>Escherichia coli</i> Biosensor for Rapid Detection of Low Cr(VI) Concentration in Environmental Water Biosensors biosensor hexavalent chromium limit of detection reporter gene |
| title | Highly Sensitive Luminescent Bioassay Using Recombinant <i>Escherichia coli</i> Biosensor for Rapid Detection of Low Cr(VI) Concentration in Environmental Water |
| title_full | Highly Sensitive Luminescent Bioassay Using Recombinant <i>Escherichia coli</i> Biosensor for Rapid Detection of Low Cr(VI) Concentration in Environmental Water |
| title_fullStr | Highly Sensitive Luminescent Bioassay Using Recombinant <i>Escherichia coli</i> Biosensor for Rapid Detection of Low Cr(VI) Concentration in Environmental Water |
| title_full_unstemmed | Highly Sensitive Luminescent Bioassay Using Recombinant <i>Escherichia coli</i> Biosensor for Rapid Detection of Low Cr(VI) Concentration in Environmental Water |
| title_short | Highly Sensitive Luminescent Bioassay Using Recombinant <i>Escherichia coli</i> Biosensor for Rapid Detection of Low Cr(VI) Concentration in Environmental Water |
| title_sort | highly sensitive luminescent bioassay using recombinant i escherichia coli i biosensor for rapid detection of low cr vi concentration in environmental water |
| topic | biosensor hexavalent chromium limit of detection reporter gene |
| url | https://www.mdpi.com/2079-6374/11/10/357 |
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