Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications
: A rapid LC-MS/MS method with good accuracy and sensitivity was developed and validated for the pharmacokinetics study of metoprolol (MP) in beagle dogs. The plasma samples were simply precipitated by methanol and then analyzed by LC-MS/MS. An Ultimate XB-C18 column (150 × 2.1 mm ID, 5 μm) was used...
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MDPI AG
2012-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/3/2663/ |
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| author | Aihua Hong Linlin Li Xiaofei Li Shaolian Fu Xiaoqi Zhang Zhiguo Ma Kelong Peng Xingli Wang Sha Li Jie Jiang |
| author_facet | Aihua Hong Linlin Li Xiaofei Li Shaolian Fu Xiaoqi Zhang Zhiguo Ma Kelong Peng Xingli Wang Sha Li Jie Jiang |
| author_sort | Aihua Hong |
| collection | DOAJ |
| description | : A rapid LC-MS/MS method with good accuracy and sensitivity was developed and validated for the pharmacokinetics study of metoprolol (MP) in beagle dogs. The plasma samples were simply precipitated by methanol and then analyzed by LC-MS/MS. An Ultimate XB-C18 column (150 × 2.1 mm ID, 5 μm) was used for separation, with methanol-water containing 0.2% formic acid (65:35, v/v) as the mobile phase at a flow rate of 0.2 mL/min. Monitoring ions of MP and internal standard (hydroxypioglitazone) were m/z 268.1/115.6 and m/z 373.1/150.2, respectively. The linear range was 3.03–416.35 ng/mL with an average correlation coefficient of 0.9996, and the limit of quantification was 3.03 ng/mL. The intra- and inter-day precision was less than 15%. At low, middle and high concentrations, the recovery, the matrix effect and the accuracy was in the range of 76.06%–95.25%, 93.67%–104.19% and 95.20%–99.96% respectively. The method was applied for the pharmacokinetics study of MP tartrate tablets (50 mg). The AUC0-t, Tmax and Cmax were respectively 919.88 ± 195.67 μg/L·h, 0.96 ± 0.33 h, 349.12 ± 78.04 ng/mL. |
| first_indexed | 2024-12-18T08:23:29Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-18T08:23:29Z |
| publishDate | 2012-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-65375fd942f344ab9361f57d04ba15742022-12-21T21:14:41ZengMDPI AGMolecules1420-30492012-03-011732663267410.3390/molecules17032663Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic ApplicationsAihua HongLinlin LiXiaofei LiShaolian FuXiaoqi ZhangZhiguo MaKelong PengXingli WangSha LiJie Jiang: A rapid LC-MS/MS method with good accuracy and sensitivity was developed and validated for the pharmacokinetics study of metoprolol (MP) in beagle dogs. The plasma samples were simply precipitated by methanol and then analyzed by LC-MS/MS. An Ultimate XB-C18 column (150 × 2.1 mm ID, 5 μm) was used for separation, with methanol-water containing 0.2% formic acid (65:35, v/v) as the mobile phase at a flow rate of 0.2 mL/min. Monitoring ions of MP and internal standard (hydroxypioglitazone) were m/z 268.1/115.6 and m/z 373.1/150.2, respectively. The linear range was 3.03–416.35 ng/mL with an average correlation coefficient of 0.9996, and the limit of quantification was 3.03 ng/mL. The intra- and inter-day precision was less than 15%. At low, middle and high concentrations, the recovery, the matrix effect and the accuracy was in the range of 76.06%–95.25%, 93.67%–104.19% and 95.20%–99.96% respectively. The method was applied for the pharmacokinetics study of MP tartrate tablets (50 mg). The AUC0-t, Tmax and Cmax were respectively 919.88 ± 195.67 μg/L·h, 0.96 ± 0.33 h, 349.12 ± 78.04 ng/mL.http://www.mdpi.com/1420-3049/17/3/2663/metoprolol tartrateLC-MS/MSpharmacokineticsprotein precipitation method |
| spellingShingle | Aihua Hong Linlin Li Xiaofei Li Shaolian Fu Xiaoqi Zhang Zhiguo Ma Kelong Peng Xingli Wang Sha Li Jie Jiang Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications Molecules metoprolol tartrate LC-MS/MS pharmacokinetics protein precipitation method |
| title | Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications |
| title_full | Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications |
| title_fullStr | Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications |
| title_full_unstemmed | Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications |
| title_short | Rapid and Sensitive LC-MS/MS Method for the Determination of Metoprolol in Beagle Dog Plasma with a Simple Protein Precipitation Treatment and Its Pharmacokinetic Applications |
| title_sort | rapid and sensitive lc ms ms method for the determination of metoprolol in beagle dog plasma with a simple protein precipitation treatment and its pharmacokinetic applications |
| topic | metoprolol tartrate LC-MS/MS pharmacokinetics protein precipitation method |
| url | http://www.mdpi.com/1420-3049/17/3/2663/ |
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