Identification and pattern of antifungal susceptibility of Candida species isolated from cases of vaginitis in a tertiary care hospital in India
Background and Objectives: Vulvovaginal candidiasis (VVC) is one of the most frequent reasons for gynecological consultations. Candida albicans is responsible in the majority of cases. Lately, VVC caused by non-albicans Candida spp. (NAC), which are resistant to routinely used antifungals, is on th...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-04-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3592 |
| _version_ | 1797840340140425216 |
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| author | Gulnaz Bashir Insha Altaf Rabia Khurshid Tufail Ahmed Aamir Ali Sofia Zaffar |
| author_facet | Gulnaz Bashir Insha Altaf Rabia Khurshid Tufail Ahmed Aamir Ali Sofia Zaffar |
| author_sort | Gulnaz Bashir |
| collection | DOAJ |
| description |
Background and Objectives: Vulvovaginal candidiasis (VVC) is one of the most frequent reasons for gynecological consultations. Candida albicans is responsible in the majority of cases. Lately, VVC caused by non-albicans Candida spp. (NAC), which are resistant to routinely used antifungals, is on the rise. This study was designed to determine the prevalence of Candida in patients suffering from vaginitis and to assess the predisposing factors along with identification of Candida species and evaluation of their susceptibility profile.
Materials and Methods: High vaginal swabs were collected from 225 women. Sample processing consisted of Gram stain and culture onto Sabouraud’s dextrose agar and HiChrom Candida Differential agar. Isolates were identified and speciated using VITEK2 Compact System. Susceptibility testing was done using VITEK2 AST-Y S08 cards and disc diffusion.
Results: Candida spp. were isolated from 94 (41.8%) of the cases. C. albicans was the predominant species (71.6%) followed by other NAC spp. (28.4%). Pregnancy and diabetes were the most frequently implicated risk factors (67.1% and 44.4%). High resistance was observed in NAC spp. as opposed to C. albicans to all antifungal agents tested.
Conclusion: Empirical therapy with routinely used antifungals can be initiated for C. albicans. In the case of NAC spp., identification should be followed by susceptibility testing.
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| first_indexed | 2024-04-09T16:13:21Z |
| format | Article |
| id | doaj.art-6538881b83024db7af3282135df693e5 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-09T16:13:21Z |
| publishDate | 2023-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-6538881b83024db7af3282135df693e52023-04-24T06:54:19ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472023-04-01152Identification and pattern of antifungal susceptibility of Candida species isolated from cases of vaginitis in a tertiary care hospital in IndiaGulnaz Bashir0Insha Altaf1Rabia Khurshid2Tufail Ahmed3Aamir Ali4Sofia Zaffar5Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, IndiaDepartment of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, IndiaDepartment of Gynecology and Obstetrics, Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, IndiaDepartment of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, IndiaDepartment of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, IndiaDepartment of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, India Background and Objectives: Vulvovaginal candidiasis (VVC) is one of the most frequent reasons for gynecological consultations. Candida albicans is responsible in the majority of cases. Lately, VVC caused by non-albicans Candida spp. (NAC), which are resistant to routinely used antifungals, is on the rise. This study was designed to determine the prevalence of Candida in patients suffering from vaginitis and to assess the predisposing factors along with identification of Candida species and evaluation of their susceptibility profile. Materials and Methods: High vaginal swabs were collected from 225 women. Sample processing consisted of Gram stain and culture onto Sabouraud’s dextrose agar and HiChrom Candida Differential agar. Isolates were identified and speciated using VITEK2 Compact System. Susceptibility testing was done using VITEK2 AST-Y S08 cards and disc diffusion. Results: Candida spp. were isolated from 94 (41.8%) of the cases. C. albicans was the predominant species (71.6%) followed by other NAC spp. (28.4%). Pregnancy and diabetes were the most frequently implicated risk factors (67.1% and 44.4%). High resistance was observed in NAC spp. as opposed to C. albicans to all antifungal agents tested. Conclusion: Empirical therapy with routinely used antifungals can be initiated for C. albicans. In the case of NAC spp., identification should be followed by susceptibility testing. https://ijm.tums.ac.ir/index.php/ijm/article/view/3592Candidiasis;Vaginitis;Resistance;Antifungals;Reproductive health |
| spellingShingle | Gulnaz Bashir Insha Altaf Rabia Khurshid Tufail Ahmed Aamir Ali Sofia Zaffar Identification and pattern of antifungal susceptibility of Candida species isolated from cases of vaginitis in a tertiary care hospital in India Iranian Journal of Microbiology Candidiasis; Vaginitis; Resistance; Antifungals; Reproductive health |
| title | Identification and pattern of antifungal susceptibility of Candida species isolated from cases of vaginitis in a tertiary care hospital in India |
| title_full | Identification and pattern of antifungal susceptibility of Candida species isolated from cases of vaginitis in a tertiary care hospital in India |
| title_fullStr | Identification and pattern of antifungal susceptibility of Candida species isolated from cases of vaginitis in a tertiary care hospital in India |
| title_full_unstemmed | Identification and pattern of antifungal susceptibility of Candida species isolated from cases of vaginitis in a tertiary care hospital in India |
| title_short | Identification and pattern of antifungal susceptibility of Candida species isolated from cases of vaginitis in a tertiary care hospital in India |
| title_sort | identification and pattern of antifungal susceptibility of candida species isolated from cases of vaginitis in a tertiary care hospital in india |
| topic | Candidiasis; Vaginitis; Resistance; Antifungals; Reproductive health |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3592 |
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