Anti-DFS70 Antibodies Among Patient and Healthy Population Cohorts in China: Results From a Multicenter Training Program Showing Spontaneous Abortion and Pediatric Systemic Autoimmune Rheumatic Diseases Are Common in Anti-DFS70 Positive Patients
ObjectiveAnti-DFS70 antibodies correlating with the nuclear dense fine speckled (DFS) pattern in the HEp-2 indirect immunofluorescence assay (IFA) are less common in patients with systemic autoimmune rheumatic disease (SARD) than in healthy subjects and their clinical associations remain elusive. We...
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Frontiers Media S.A.
2020-10-01
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.562138/full |
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| author | Bing Zheng Zhiqing Wang Rodrigo A. Mora Aiping Liu Chihui Li Dengtao Liu Fuying Zhai Huiyuan Liu Huiyun Gong Jiaye Zhou Jing Liu Li Chen Lijun Wu Lin Yuan Lina Ying Loujian Jie Meifang He Meng Hao Ping Xu Qiuwei Lu Shanshan Han Shishi Chen Shuimian Chen Shunfei Zhu Weihua Sun Xiaoying Guo Yajuan Chen Yan Wang Yemin Qu Zhen Li Zhenzhen Niu Zhongyan Han Edward K. L. Chan |
| author_facet | Bing Zheng Zhiqing Wang Rodrigo A. Mora Aiping Liu Chihui Li Dengtao Liu Fuying Zhai Huiyuan Liu Huiyun Gong Jiaye Zhou Jing Liu Li Chen Lijun Wu Lin Yuan Lina Ying Loujian Jie Meifang He Meng Hao Ping Xu Qiuwei Lu Shanshan Han Shishi Chen Shuimian Chen Shunfei Zhu Weihua Sun Xiaoying Guo Yajuan Chen Yan Wang Yemin Qu Zhen Li Zhenzhen Niu Zhongyan Han Edward K. L. Chan |
| author_sort | Bing Zheng |
| collection | DOAJ |
| description | ObjectiveAnti-DFS70 antibodies correlating with the nuclear dense fine speckled (DFS) pattern in the HEp-2 indirect immunofluorescence assay (IFA) are less common in patients with systemic autoimmune rheumatic disease (SARD) than in healthy subjects and their clinical associations remain elusive. We hosted a multi-center HEp-2 IFA training program to improve the ability of clinical laboratories to recognize the DFS pattern and to investigate the prevalence and relevance of anti-DFS70 antibodies.MethodsDFS pattern sera identified by HEp-2 IFA in 29 centers in China were redirected to a central laboratory for anti-DFS70 testing by line immunoblot assay (LIA), enzyme-linked immunosorbent assay (ELISA), and IFA with HEp-2 ELITE/DFS70-KO substrate. Anti-extractable nuclear antigen antibodies were measured by LIA and the clinical relevance was examined in adult and pediatric patients.ResultsHEp-2 IFA positive rate and DFS pattern in positive sera were 36.2% (34,417/95,131) and 1.7% (582/34,417) in the patient cohort, and 10.0% (423/4,234) and 7.8% (33/423) in a healthy population, respectively. Anti-DFS70 prevalence among sera presenting the DFS pattern was 96.0, 93.7, and 49.6% by ELISA, LIA, and HEp-2 ELITE, respectively. 15.5% (52/336) of adult and 50.0% (20/40) of pediatric anti-DFS70 positive patients were diagnosed with SARD. Diseases most common in anti-DFS70 positive patients were spontaneous abortion (28.0%) in adults and juvenile idiopathic arthritis (22.5%) in pediatric patients.ConclusionAccurate DFS pattern identification increased the detection rate of anti-DFS70 antibodies by ELISA and LIA. Anti-DFS70 antibodies are remarkably high in cases of spontaneous abortion and in pediatric SARD patients, but not prevalent in adult SARD patients. |
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| spelling | doaj.art-653bc94e53094bb892cfe48bbea36fc32022-12-21T19:02:06ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-10-011110.3389/fimmu.2020.562138562138Anti-DFS70 Antibodies Among Patient and Healthy Population Cohorts in China: Results From a Multicenter Training Program Showing Spontaneous Abortion and Pediatric Systemic Autoimmune Rheumatic Diseases Are Common in Anti-DFS70 Positive PatientsBing Zheng0Zhiqing Wang1Rodrigo A. Mora2Aiping Liu3Chihui Li4Dengtao Liu5Fuying Zhai6Huiyuan Liu7Huiyun Gong8Jiaye Zhou9Jing Liu10Li Chen11Lijun Wu12Lin Yuan13Lina Ying14Loujian Jie15Meifang He16Meng Hao17Ping Xu18Qiuwei Lu19Shanshan Han20Shishi Chen21Shuimian Chen22Shunfei Zhu23Weihua Sun24Xiaoying Guo25Yajuan Chen26Yan Wang27Yemin Qu28Zhen Li29Zhenzhen Niu30Zhongyan Han31Edward K. L. Chan32Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Oral Biology, University of Florida, Gainesville, FL, United StatesDepartment of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Laboratory Medicine, Jinhua People’s Hospital, Jinhua, ChinaDepartment of Clinical Laboratory, Linyi People’s Hospital, Linyi, ChinaDepartment of Laboratory Medicine, People’s Hospital of Rongcheng, Rongcheng, ChinaDepartment of Rheumatology Laboratory, Futian District Hospital of Rheumatism, Shenzhen, ChinaDepartment of Laboratory Medicine, Ruijing Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China0Department of Laboratory Medicine, General Hospital of Northern Theater Command, Shengyang, China1Department of Laboratory Medicine, Zhejiang Provincial Hospital of TCM, The First Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou, China2Department of Rheumatology Laboratory, People’s Hospital of Xinjiang Ugyur Autonomous Region, Urumqi, China3Department of Laboratory Medicine, Weihai Central Hospital, Weihai, China4Department of Clinical Laboratory, Ninbo No.6 Hospital, Ninbo, China5Department of Laboratory Medicine, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, China6Department of Laboratory Medicine, The Third People’s Hospital of Changzhou, Changzhou, China7Department of Laboratory Medicine, Jiujiang First People’s Hospital, Jiujiang, China8Department of Laboratory Medicine, Obstetrics & Gynecology Hospital Affiliated to Fudan University, Shanghai, China9Department of Laboratory Medicine, The People’s Hospital of Guangxi Zhuang Autonomous Regain, Nanning, China0Department of Laboratory Medicine, People’s Hospital of Sanmen, Taizhou, China1Department of Laboratory Medicine, The Fifth Affiliated Hospital Sun Yat-Sen University, Zhuhai, China2Department of Laboratory Medicine, Zhenggu Hospital, School of Medicine, Fujian University of Traditional Chinese Medicine, Quanzhou, China3Department of Laboratory Medicine, Zibo Maternal and Child Health Hospital, Zibo, China4Department of Laboratory Medicine, The 940th Hospital of PLA Joint Logistics Support Force, Lanzhou, China5Department of Laboratory Medicine, Daqing Oilfield General Hospital, Daqing, China6Departments of Microbiological and Immunology, 3201 Hospital, Hanzhong, China7Department of Laboratory Medicine, The Red Cross Hospital, Xining, China8Department of Laboratory Medicine, Weihai Municipal Hospital, Shandong University, Weihai, China9Department of Laboratory Medicine, Yili Kazak Autonomous Prefecture Hospital of Traditional Chinese Medicine, Yili, China0Department of Laboratory Medicine, Yinzhou People’s Hospital, Ningbo, China1Center of Pathology and Clinical Laboratory, Sir Run Run Hospital, Nanjing Medical University, Nanjing, ChinaDepartment of Oral Biology, University of Florida, Gainesville, FL, United StatesObjectiveAnti-DFS70 antibodies correlating with the nuclear dense fine speckled (DFS) pattern in the HEp-2 indirect immunofluorescence assay (IFA) are less common in patients with systemic autoimmune rheumatic disease (SARD) than in healthy subjects and their clinical associations remain elusive. We hosted a multi-center HEp-2 IFA training program to improve the ability of clinical laboratories to recognize the DFS pattern and to investigate the prevalence and relevance of anti-DFS70 antibodies.MethodsDFS pattern sera identified by HEp-2 IFA in 29 centers in China were redirected to a central laboratory for anti-DFS70 testing by line immunoblot assay (LIA), enzyme-linked immunosorbent assay (ELISA), and IFA with HEp-2 ELITE/DFS70-KO substrate. Anti-extractable nuclear antigen antibodies were measured by LIA and the clinical relevance was examined in adult and pediatric patients.ResultsHEp-2 IFA positive rate and DFS pattern in positive sera were 36.2% (34,417/95,131) and 1.7% (582/34,417) in the patient cohort, and 10.0% (423/4,234) and 7.8% (33/423) in a healthy population, respectively. Anti-DFS70 prevalence among sera presenting the DFS pattern was 96.0, 93.7, and 49.6% by ELISA, LIA, and HEp-2 ELITE, respectively. 15.5% (52/336) of adult and 50.0% (20/40) of pediatric anti-DFS70 positive patients were diagnosed with SARD. Diseases most common in anti-DFS70 positive patients were spontaneous abortion (28.0%) in adults and juvenile idiopathic arthritis (22.5%) in pediatric patients.ConclusionAccurate DFS pattern identification increased the detection rate of anti-DFS70 antibodies by ELISA and LIA. Anti-DFS70 antibodies are remarkably high in cases of spontaneous abortion and in pediatric SARD patients, but not prevalent in adult SARD patients.https://www.frontiersin.org/article/10.3389/fimmu.2020.562138/fullantinuclear antibodiesDFS70pediatric rheumatic diseasespontaneous abortionsystemic autoimmune rheumatic disease |
| spellingShingle | Bing Zheng Zhiqing Wang Rodrigo A. Mora Aiping Liu Chihui Li Dengtao Liu Fuying Zhai Huiyuan Liu Huiyun Gong Jiaye Zhou Jing Liu Li Chen Lijun Wu Lin Yuan Lina Ying Loujian Jie Meifang He Meng Hao Ping Xu Qiuwei Lu Shanshan Han Shishi Chen Shuimian Chen Shunfei Zhu Weihua Sun Xiaoying Guo Yajuan Chen Yan Wang Yemin Qu Zhen Li Zhenzhen Niu Zhongyan Han Edward K. L. Chan Anti-DFS70 Antibodies Among Patient and Healthy Population Cohorts in China: Results From a Multicenter Training Program Showing Spontaneous Abortion and Pediatric Systemic Autoimmune Rheumatic Diseases Are Common in Anti-DFS70 Positive Patients Frontiers in Immunology antinuclear antibodies DFS70 pediatric rheumatic disease spontaneous abortion systemic autoimmune rheumatic disease |
| title | Anti-DFS70 Antibodies Among Patient and Healthy Population Cohorts in China: Results From a Multicenter Training Program Showing Spontaneous Abortion and Pediatric Systemic Autoimmune Rheumatic Diseases Are Common in Anti-DFS70 Positive Patients |
| title_full | Anti-DFS70 Antibodies Among Patient and Healthy Population Cohorts in China: Results From a Multicenter Training Program Showing Spontaneous Abortion and Pediatric Systemic Autoimmune Rheumatic Diseases Are Common in Anti-DFS70 Positive Patients |
| title_fullStr | Anti-DFS70 Antibodies Among Patient and Healthy Population Cohorts in China: Results From a Multicenter Training Program Showing Spontaneous Abortion and Pediatric Systemic Autoimmune Rheumatic Diseases Are Common in Anti-DFS70 Positive Patients |
| title_full_unstemmed | Anti-DFS70 Antibodies Among Patient and Healthy Population Cohorts in China: Results From a Multicenter Training Program Showing Spontaneous Abortion and Pediatric Systemic Autoimmune Rheumatic Diseases Are Common in Anti-DFS70 Positive Patients |
| title_short | Anti-DFS70 Antibodies Among Patient and Healthy Population Cohorts in China: Results From a Multicenter Training Program Showing Spontaneous Abortion and Pediatric Systemic Autoimmune Rheumatic Diseases Are Common in Anti-DFS70 Positive Patients |
| title_sort | anti dfs70 antibodies among patient and healthy population cohorts in china results from a multicenter training program showing spontaneous abortion and pediatric systemic autoimmune rheumatic diseases are common in anti dfs70 positive patients |
| topic | antinuclear antibodies DFS70 pediatric rheumatic disease spontaneous abortion systemic autoimmune rheumatic disease |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2020.562138/full |
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