Genome-wide association study of Senegalese sorghum seedlings responding to a Texas isolate of Colletotrichum sublineola
Abstract Colletotrichum sublineola is a destructive fungal pathogen that causes anthracnose in sorghum. Senegalese sorghum germplasm is currently being considered as an option of sources for genetic resistance. In a recent study, Senegalese sorghum accessions were evaluated for response to a mixture...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-16844-6 |
| _version_ | 1818162636416614400 |
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| author | Ezekiel Ahn Coumba Fall Louis K. Prom Clint Magill |
| author_facet | Ezekiel Ahn Coumba Fall Louis K. Prom Clint Magill |
| author_sort | Ezekiel Ahn |
| collection | DOAJ |
| description | Abstract Colletotrichum sublineola is a destructive fungal pathogen that causes anthracnose in sorghum. Senegalese sorghum germplasm is currently being considered as an option of sources for genetic resistance. In a recent study, Senegalese sorghum accessions were evaluated for response to a mixture of Texas isolates of C. sublineola at the 8-leaf stage in the greenhouse. As a comparison, 159 Senegalese sorghum accessions at the 1-leaf developmental stage were evaluated against a single Texas isolate of C. sublineola (FSP53) using an excised-leaf assay. A genome-wide association study (GWAS) was conducted based on the phenotypic data acquired to discover genetic variation associated with response to C. sublineola using 193,727 single nucleotide polymorphisms (SNPs) throughout the genome. Sorghum seedlings tended to be more resistant when compared with sorghum plants inoculated at the 8-leaf stage in the greenhouse in previous experiments. Based on the highest score evaluated in the 1-leaf developmental stage excised leaf assay for each accession, 16 accessions were labeled as susceptible. GWAS identified the SNP locus S01_72868925 that is associated with protein kinase domain // Leucine rich repeat N-terminal domain at a level of confidence that surpassed Bonferroni correction. Along with the SNP locus S01_72868925, other top SNP loci were also associated with genes that are known to play critical roles in plant defense or plant stress responses. |
| first_indexed | 2024-12-11T16:36:49Z |
| format | Article |
| id | doaj.art-653c6a3bc3754fc3934b5998a97be0f4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-11T16:36:49Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-653c6a3bc3754fc3934b5998a97be0f42022-12-22T00:58:25ZengNature PortfolioScientific Reports2045-23222022-07-011211710.1038/s41598-022-16844-6Genome-wide association study of Senegalese sorghum seedlings responding to a Texas isolate of Colletotrichum sublineolaEzekiel Ahn0Coumba Fall1Louis K. Prom2Clint Magill3Department of Plant Pathology and Microbiology, Texas A&M UniversityDepartment of Plant Pathology and Microbiology, Texas A&M UniversityUSDA-ARS Southern Plains Agricultural Research CenterDepartment of Plant Pathology and Microbiology, Texas A&M UniversityAbstract Colletotrichum sublineola is a destructive fungal pathogen that causes anthracnose in sorghum. Senegalese sorghum germplasm is currently being considered as an option of sources for genetic resistance. In a recent study, Senegalese sorghum accessions were evaluated for response to a mixture of Texas isolates of C. sublineola at the 8-leaf stage in the greenhouse. As a comparison, 159 Senegalese sorghum accessions at the 1-leaf developmental stage were evaluated against a single Texas isolate of C. sublineola (FSP53) using an excised-leaf assay. A genome-wide association study (GWAS) was conducted based on the phenotypic data acquired to discover genetic variation associated with response to C. sublineola using 193,727 single nucleotide polymorphisms (SNPs) throughout the genome. Sorghum seedlings tended to be more resistant when compared with sorghum plants inoculated at the 8-leaf stage in the greenhouse in previous experiments. Based on the highest score evaluated in the 1-leaf developmental stage excised leaf assay for each accession, 16 accessions were labeled as susceptible. GWAS identified the SNP locus S01_72868925 that is associated with protein kinase domain // Leucine rich repeat N-terminal domain at a level of confidence that surpassed Bonferroni correction. Along with the SNP locus S01_72868925, other top SNP loci were also associated with genes that are known to play critical roles in plant defense or plant stress responses.https://doi.org/10.1038/s41598-022-16844-6 |
| spellingShingle | Ezekiel Ahn Coumba Fall Louis K. Prom Clint Magill Genome-wide association study of Senegalese sorghum seedlings responding to a Texas isolate of Colletotrichum sublineola Scientific Reports |
| title | Genome-wide association study of Senegalese sorghum seedlings responding to a Texas isolate of Colletotrichum sublineola |
| title_full | Genome-wide association study of Senegalese sorghum seedlings responding to a Texas isolate of Colletotrichum sublineola |
| title_fullStr | Genome-wide association study of Senegalese sorghum seedlings responding to a Texas isolate of Colletotrichum sublineola |
| title_full_unstemmed | Genome-wide association study of Senegalese sorghum seedlings responding to a Texas isolate of Colletotrichum sublineola |
| title_short | Genome-wide association study of Senegalese sorghum seedlings responding to a Texas isolate of Colletotrichum sublineola |
| title_sort | genome wide association study of senegalese sorghum seedlings responding to a texas isolate of colletotrichum sublineola |
| url | https://doi.org/10.1038/s41598-022-16844-6 |
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