Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines
Pholiota adiposa is a kind of edible mushroom which has long been known for its health care applications. To reveal the exact mechanism of its protective functions in humans, in this study we isolated and identified the active compound PB3 of P. adiposa for the first time by a combination of chromat...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/18/2/1775 |
| _version_ | 1818960348516974592 |
|---|---|
| author | Chang Rong Wang Wen Tao Qiao Fang Liu Ye Ni Zhang |
| author_facet | Chang Rong Wang Wen Tao Qiao Fang Liu Ye Ni Zhang |
| author_sort | Chang Rong Wang |
| collection | DOAJ |
| description | Pholiota adiposa is a kind of edible mushroom which has long been known for its health care applications. To reveal the exact mechanism of its protective functions in humans, in this study we isolated and identified the active compound PB3 of P. adiposa for the first time by a combination of chromatography techniques, including NKA macroporous resin and Sephadex G-15. PB3, with molecular mass of 267.2 Da and molecular formula of C10H13N5O4 discovered by mass spectrum (MS) was identified to be adenosine. Mice were injected intraperitoneally with purified fraction PB3. Seven days after injection, we found a 1.5-fold increase of IL10 at the mRNA level, while a down regulated expression of IL-2, IL-6 and IFN-γ to 49.0%, 56.9% and 73.4%, respectively, was detected in spleen by real-time quantitative PCR. What’s more, SOD expression level was significantly increased by 1.6-fold compared to control. Fraction PB3 displayed anti-inflammatory potency and heightened SOD activity on the transcriptional level, which could be considered of further pharmaceutical or medication value. |
| first_indexed | 2024-12-20T11:56:06Z |
| format | Article |
| id | doaj.art-653d492f9db64240875b3d5b264ab3ab |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T11:56:06Z |
| publishDate | 2013-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-653d492f9db64240875b3d5b264ab3ab2022-12-21T19:41:38ZengMDPI AGMolecules1420-30492013-01-011821775178210.3390/molecules18021775Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory CytokinesChang Rong WangWen Tao QiaoFang LiuYe Ni ZhangPholiota adiposa is a kind of edible mushroom which has long been known for its health care applications. To reveal the exact mechanism of its protective functions in humans, in this study we isolated and identified the active compound PB3 of P. adiposa for the first time by a combination of chromatography techniques, including NKA macroporous resin and Sephadex G-15. PB3, with molecular mass of 267.2 Da and molecular formula of C10H13N5O4 discovered by mass spectrum (MS) was identified to be adenosine. Mice were injected intraperitoneally with purified fraction PB3. Seven days after injection, we found a 1.5-fold increase of IL10 at the mRNA level, while a down regulated expression of IL-2, IL-6 and IFN-γ to 49.0%, 56.9% and 73.4%, respectively, was detected in spleen by real-time quantitative PCR. What’s more, SOD expression level was significantly increased by 1.6-fold compared to control. Fraction PB3 displayed anti-inflammatory potency and heightened SOD activity on the transcriptional level, which could be considered of further pharmaceutical or medication value.http://www.mdpi.com/1420-3049/18/2/1775adenosinePholiota adiposaSODimmunomodulatory cytokines |
| spellingShingle | Chang Rong Wang Wen Tao Qiao Fang Liu Ye Ni Zhang Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines Molecules adenosine Pholiota adiposa SOD immunomodulatory cytokines |
| title | Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines |
| title_full | Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines |
| title_fullStr | Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines |
| title_full_unstemmed | Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines |
| title_short | Effects of Adenosine Extract from Pholiota adiposa (Fr.) Quel on mRNA Expressions of Superoxide Dismutase and Immunomodulatory Cytokines |
| title_sort | effects of adenosine extract from pholiota adiposa fr quel on mrna expressions of superoxide dismutase and immunomodulatory cytokines |
| topic | adenosine Pholiota adiposa SOD immunomodulatory cytokines |
| url | http://www.mdpi.com/1420-3049/18/2/1775 |
| work_keys_str_mv | AT changrongwang effectsofadenosineextractfrompholiotaadiposafrquelonmrnaexpressionsofsuperoxidedismutaseandimmunomodulatorycytokines AT wentaoqiao effectsofadenosineextractfrompholiotaadiposafrquelonmrnaexpressionsofsuperoxidedismutaseandimmunomodulatorycytokines AT fangliu effectsofadenosineextractfrompholiotaadiposafrquelonmrnaexpressionsofsuperoxidedismutaseandimmunomodulatorycytokines AT yenizhang effectsofadenosineextractfrompholiotaadiposafrquelonmrnaexpressionsofsuperoxidedismutaseandimmunomodulatorycytokines |