Fluorescently tagged Lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epithelium
Summary Development of epithelial cell polarity is a highly dynamic process, and often established by the sequential recruitment of conserved protein complexes, such as the Par or the Crumbs (Crb) complex. However, detailed insights into the refinement of polarity and the formation of the complexes...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Company of Biologists
2013-07-01
|
| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/2/9/867 |
| _version_ | 1818577013852602368 |
|---|---|
| author | Marta Luz Elisabeth Knust |
| author_facet | Marta Luz Elisabeth Knust |
| author_sort | Marta Luz |
| collection | DOAJ |
| description | Summary
Development of epithelial cell polarity is a highly dynamic process, and often established by the sequential recruitment of conserved protein complexes, such as the Par or the Crumbs (Crb) complex. However, detailed insights into the refinement of polarity and the formation of the complexes are still lacking. Here, we established fluorescently tagged Lin7c, a core member of the Crb complex, as an ideal tool to follow development of polarity in zebrafish epithelia. We find that in gastrula stages, RFP-Lin7c is found in the cytosol of the enveloping layer, while Pard3-GFP is already polarized at this stage. During development of the retinal epithelium, RFP-Lin7c localization is refined from being cytosolic at 14 hours post fertilization (hpf) to almost entirely apical in cells of the eye cup at 28 hpf. This apical Lin7c localization depends on the Crb complex members Oko meduzy and Nagie oko. Thus, fluorescently tagged Lin7c can be used in a broad range of epithelia to follow polarity maturation in vivo and specifically to elucidate the sequence of events determining Crb complex-mediated polarity. |
| first_indexed | 2024-12-16T06:23:10Z |
| format | Article |
| id | doaj.art-654042a1c685473e94cfede5509e3ffd |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-16T06:23:10Z |
| publishDate | 2013-07-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-654042a1c685473e94cfede5509e3ffd2022-12-21T22:41:04ZengThe Company of BiologistsBiology Open2046-63902013-07-012986787110.1242/bio.2013537120135371Fluorescently tagged Lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epitheliumMarta LuzElisabeth KnustSummary Development of epithelial cell polarity is a highly dynamic process, and often established by the sequential recruitment of conserved protein complexes, such as the Par or the Crumbs (Crb) complex. However, detailed insights into the refinement of polarity and the formation of the complexes are still lacking. Here, we established fluorescently tagged Lin7c, a core member of the Crb complex, as an ideal tool to follow development of polarity in zebrafish epithelia. We find that in gastrula stages, RFP-Lin7c is found in the cytosol of the enveloping layer, while Pard3-GFP is already polarized at this stage. During development of the retinal epithelium, RFP-Lin7c localization is refined from being cytosolic at 14 hours post fertilization (hpf) to almost entirely apical in cells of the eye cup at 28 hpf. This apical Lin7c localization depends on the Crb complex members Oko meduzy and Nagie oko. Thus, fluorescently tagged Lin7c can be used in a broad range of epithelia to follow polarity maturation in vivo and specifically to elucidate the sequence of events determining Crb complex-mediated polarity.http://bio.biologists.org/content/2/9/867PolarityNeuroepitheliaRetinaCrumbsZebrafish |
| spellingShingle | Marta Luz Elisabeth Knust Fluorescently tagged Lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epithelium Biology Open Polarity Neuroepithelia Retina Crumbs Zebrafish |
| title | Fluorescently tagged Lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epithelium |
| title_full | Fluorescently tagged Lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epithelium |
| title_fullStr | Fluorescently tagged Lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epithelium |
| title_full_unstemmed | Fluorescently tagged Lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epithelium |
| title_short | Fluorescently tagged Lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epithelium |
| title_sort | fluorescently tagged lin7c is a dynamic marker for polarity maturation in the zebrafish retinal epithelium |
| topic | Polarity Neuroepithelia Retina Crumbs Zebrafish |
| url | http://bio.biologists.org/content/2/9/867 |
| work_keys_str_mv | AT martaluz fluorescentlytaggedlin7cisadynamicmarkerforpolaritymaturationinthezebrafishretinalepithelium AT elisabethknust fluorescentlytaggedlin7cisadynamicmarkerforpolaritymaturationinthezebrafishretinalepithelium |