A simplified protocol for performing MAGMA/H-MAGMA gene set analysis utilizing high-performance computing environments
Summary: Here, we present a quick-start protocol to perform generalized gene-set analysis of GWAS data on a metaset of gene lists generated by upstream pipelines, such as differential expression analysis, using the Multi-marker Analysis of GenoMic Annotation (MAGMA) software package and Hi-C coupled...
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Format: | Article |
Language: | English |
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Elsevier
2022-03-01
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Series: | STAR Protocols |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721007899 |
Summary: | Summary: Here, we present a quick-start protocol to perform generalized gene-set analysis of GWAS data on a metaset of gene lists generated by upstream pipelines, such as differential expression analysis, using the Multi-marker Analysis of GenoMic Annotation (MAGMA) software package and Hi-C coupled H-MAGMA annotation data (de Leeuw et al., 2015; Sey et al., 2020). We specifically tailor the steps and operations to meet the multithreading capability in modern computers, a feature nowadays shared by personal computers and high-performance clusters alike.For complete details on the use and execution of this profile, please refer to Yao et al. (2021). |
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ISSN: | 2666-1667 |