Evening Primrose Oil Ameliorates Hyperleptinemia and Reproductive Hormone Disturbances in Obese Female Rats: Impact on Estrus Cyclicity
Obesity is a public health burden disturbing all body functions and reproductive hormones. As obesity increases among females, there will be a rising challenge to physicians in care from fertility problems. Evening primrose oil (EPR oil) contains essential fatty acids including omega-6 linoleic acid...
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fendo.2019.00942/full |
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| author | Hebatallah H. Atteia Hebatallah H. Atteia Sharifa Alzahrani Nagla A. El-Sherbeeny Amal M. Youssef Amal M. Youssef Noha E. Farag Eman T. Mehanna Reda Elhawary Gehan A. Ibrahim Amr Elmistekawy Sawsan A. Zaitone Sawsan A. Zaitone |
| author_facet | Hebatallah H. Atteia Hebatallah H. Atteia Sharifa Alzahrani Nagla A. El-Sherbeeny Amal M. Youssef Amal M. Youssef Noha E. Farag Eman T. Mehanna Reda Elhawary Gehan A. Ibrahim Amr Elmistekawy Sawsan A. Zaitone Sawsan A. Zaitone |
| author_sort | Hebatallah H. Atteia |
| collection | DOAJ |
| description | Obesity is a public health burden disturbing all body functions and reproductive hormones. As obesity increases among females, there will be a rising challenge to physicians in care from fertility problems. Evening primrose oil (EPR oil) contains essential fatty acids including omega-6 linoleic acid with strong anti-inflammatory activity. Since EPR oil has utility in alleviating dysmenorrhea, this study aimed to ascertain its modulatory effect on systemic inflammation, reproductive hormones and estrus cycle irregularity in female obese rats. Thirty-two female rats were distributed to 4 groups: (i) normal, (ii) dietary obese-control female rats, and (iii and iv) dietary obese female rats treated with EPR oil (5 or 10 g/kg). Rats were examined for estrus regularity by taking vaginal smears daily during the last 2 weeks of the experiment. Serum level of insulin, leptin, adiponectin, and inflammatory cytokines was measured. In addition, serum lipid profile, and liver enzyme activities were estimated. Adipose tissues were taken for histopathologic examination as well as determination of gene expression for leptin, leptin receptors, adiponectin, and visfatin. Obese rats exhibited significant weight gain (90.69 ± 8.9), irregular prolonged estrus cycles (83.33%), increased serum levels of insulin, leptin, prolactin and testosterone and decreased gonadotropin levels. EPR oil exhibited a curative effect on obesity-related irregularity in estrus cycle and ovarian pathology. The underlying molecular mechanism may be related to reduction of systemic inflammation, alleviating insulin resistance and modulation of adipokine expression. EPR oil may be considered as a promising therapeutic intervention against obesity-related female hormonal disturbances and estrus irregularity. |
| first_indexed | 2024-04-14T06:22:18Z |
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| id | doaj.art-654a00fcc34147f7a3d7e2d3b29a0e7e |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-14T06:22:18Z |
| publishDate | 2020-01-01 |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-654a00fcc34147f7a3d7e2d3b29a0e7e2022-12-22T02:07:58ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922020-01-011010.3389/fendo.2019.00942502229Evening Primrose Oil Ameliorates Hyperleptinemia and Reproductive Hormone Disturbances in Obese Female Rats: Impact on Estrus CyclicityHebatallah H. Atteia0Hebatallah H. Atteia1Sharifa Alzahrani2Nagla A. El-Sherbeeny3Amal M. Youssef4Amal M. Youssef5Noha E. Farag6Eman T. Mehanna7Reda Elhawary8Gehan A. Ibrahim9Amr Elmistekawy10Sawsan A. Zaitone11Sawsan A. Zaitone12Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Tabuk, Tabuk, Saudi ArabiaDepartment of Biochemistry, Faculty of Pharmacy, Zagazig University, Zagazig, EgyptPharmacology Department, Faculty of Medicine, University of Tabuk, Tabuk, Saudi ArabiaDepartment of Pharmacology, Faculty of Medicine, Suez Canal University, Ismailia, EgyptDepartment of Physiology, Faculty of Medicine, Taibah University, Medina, Saudi ArabiaDepartment of Physiology, Faculty of Medicine, Suez Canal University, Ismailia, EgyptDepartment of Physiology, Faculty of Medicine, Suez Canal University, Ismailia, EgyptDepartment of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, EgyptDepartment of Pathology, Faculty of Medicine, Al-Azhar University, Cairo, EgyptClinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt0Department of Internal Medicine, Gastroenterology Division, Faculty of Medicine, Al-Azhar University, Cairo, Egypt1Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Tabuk, Tabuk, Saudi Arabia2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia, EgyptObesity is a public health burden disturbing all body functions and reproductive hormones. As obesity increases among females, there will be a rising challenge to physicians in care from fertility problems. Evening primrose oil (EPR oil) contains essential fatty acids including omega-6 linoleic acid with strong anti-inflammatory activity. Since EPR oil has utility in alleviating dysmenorrhea, this study aimed to ascertain its modulatory effect on systemic inflammation, reproductive hormones and estrus cycle irregularity in female obese rats. Thirty-two female rats were distributed to 4 groups: (i) normal, (ii) dietary obese-control female rats, and (iii and iv) dietary obese female rats treated with EPR oil (5 or 10 g/kg). Rats were examined for estrus regularity by taking vaginal smears daily during the last 2 weeks of the experiment. Serum level of insulin, leptin, adiponectin, and inflammatory cytokines was measured. In addition, serum lipid profile, and liver enzyme activities were estimated. Adipose tissues were taken for histopathologic examination as well as determination of gene expression for leptin, leptin receptors, adiponectin, and visfatin. Obese rats exhibited significant weight gain (90.69 ± 8.9), irregular prolonged estrus cycles (83.33%), increased serum levels of insulin, leptin, prolactin and testosterone and decreased gonadotropin levels. EPR oil exhibited a curative effect on obesity-related irregularity in estrus cycle and ovarian pathology. The underlying molecular mechanism may be related to reduction of systemic inflammation, alleviating insulin resistance and modulation of adipokine expression. EPR oil may be considered as a promising therapeutic intervention against obesity-related female hormonal disturbances and estrus irregularity.https://www.frontiersin.org/article/10.3389/fendo.2019.00942/fulldietary obese female ratsestrus cyclicityevening primrose oilhyperleptinemiareproductive hormone disturbancesinsulin resistance |
| spellingShingle | Hebatallah H. Atteia Hebatallah H. Atteia Sharifa Alzahrani Nagla A. El-Sherbeeny Amal M. Youssef Amal M. Youssef Noha E. Farag Eman T. Mehanna Reda Elhawary Gehan A. Ibrahim Amr Elmistekawy Sawsan A. Zaitone Sawsan A. Zaitone Evening Primrose Oil Ameliorates Hyperleptinemia and Reproductive Hormone Disturbances in Obese Female Rats: Impact on Estrus Cyclicity Frontiers in Endocrinology dietary obese female rats estrus cyclicity evening primrose oil hyperleptinemia reproductive hormone disturbances insulin resistance |
| title | Evening Primrose Oil Ameliorates Hyperleptinemia and Reproductive Hormone Disturbances in Obese Female Rats: Impact on Estrus Cyclicity |
| title_full | Evening Primrose Oil Ameliorates Hyperleptinemia and Reproductive Hormone Disturbances in Obese Female Rats: Impact on Estrus Cyclicity |
| title_fullStr | Evening Primrose Oil Ameliorates Hyperleptinemia and Reproductive Hormone Disturbances in Obese Female Rats: Impact on Estrus Cyclicity |
| title_full_unstemmed | Evening Primrose Oil Ameliorates Hyperleptinemia and Reproductive Hormone Disturbances in Obese Female Rats: Impact on Estrus Cyclicity |
| title_short | Evening Primrose Oil Ameliorates Hyperleptinemia and Reproductive Hormone Disturbances in Obese Female Rats: Impact on Estrus Cyclicity |
| title_sort | evening primrose oil ameliorates hyperleptinemia and reproductive hormone disturbances in obese female rats impact on estrus cyclicity |
| topic | dietary obese female rats estrus cyclicity evening primrose oil hyperleptinemia reproductive hormone disturbances insulin resistance |
| url | https://www.frontiersin.org/article/10.3389/fendo.2019.00942/full |
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