Assessment of the Optimal Stimulus Pattern to Achieve Rapid Dorsal Hippocampal Kindling in Rats
Introduction: Although hippocampus is the most famous brain area involved in temporal lobe epilepsy, hippocampal kindling (HK) develops very slowly. Hence, rapid kindling is usually preferred to the traditional kindling and it is widely used. In this article we aimed at finding the optimal stimulus...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2015-04-01
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| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.iums.ac.ir/browse.php?a_code=A-10-662-2&slc_lang=en&sid=1 |
| _version_ | 1797282492594520064 |
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| author | Fataemeh Etemadi Mohammad Sayyah Hamid Gholami Pourbadi Vahab Babapour |
| author_facet | Fataemeh Etemadi Mohammad Sayyah Hamid Gholami Pourbadi Vahab Babapour |
| author_sort | Fataemeh Etemadi |
| collection | DOAJ |
| description | Introduction: Although hippocampus is the most famous brain area involved in temporal lobe
epilepsy, hippocampal kindling (HK) develops very slowly. Hence, rapid kindling is usually
preferred to the traditional kindling and it is widely used. In this article we aimed at finding the
optimal stimulus pattern, which yields the fastest HK rate. Methods: Stimulus patterns with different duration (2, 3, 5 and 10 s) and inter-train interval (ITI)
(5, 10 and 30 min) as well as number of trains in 24 h (8 and 12) were exerted to rats’ dorsal
hippocampus. The stimuli were continued until appearance of 3 consecutive generalized seizures
or maximum 7 days stimulations. Results: While the protocol with train duration of 10 s and ITI of 30 min caused the fastest
kindling rate and the most growth of afterdischarges, the protocol with train duration of 5 s and
ITI of 5 min was the most time-consuming protocol among protocols tested. Discussion: Rapid HK develops with a time course of days compared to weeks in traditional
kindling. Train duration and inter-train interval are key factors for rapid HK. Among the patterns,
12 trains/24h of 50Hz monophasic square wave with 10 s duration and 30 min interval between
trains, is the best stimulus pattern for eliciting rapid dorsal HK. |
| first_indexed | 2024-03-07T17:14:51Z |
| format | Article |
| id | doaj.art-654b1d2394e4422b87821c7f667118ef |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:14:51Z |
| publishDate | 2015-04-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-654b1d2394e4422b87821c7f667118ef2024-03-02T23:28:18ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422015-04-0162107112Assessment of the Optimal Stimulus Pattern to Achieve Rapid Dorsal Hippocampal Kindling in RatsFataemeh Etemadi0Mohammad Sayyah1Hamid Gholami Pourbadi2Vahab Babapour3 Department of Physiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Department of Physiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran Introduction: Although hippocampus is the most famous brain area involved in temporal lobe epilepsy, hippocampal kindling (HK) develops very slowly. Hence, rapid kindling is usually preferred to the traditional kindling and it is widely used. In this article we aimed at finding the optimal stimulus pattern, which yields the fastest HK rate. Methods: Stimulus patterns with different duration (2, 3, 5 and 10 s) and inter-train interval (ITI) (5, 10 and 30 min) as well as number of trains in 24 h (8 and 12) were exerted to rats’ dorsal hippocampus. The stimuli were continued until appearance of 3 consecutive generalized seizures or maximum 7 days stimulations. Results: While the protocol with train duration of 10 s and ITI of 30 min caused the fastest kindling rate and the most growth of afterdischarges, the protocol with train duration of 5 s and ITI of 5 min was the most time-consuming protocol among protocols tested. Discussion: Rapid HK develops with a time course of days compared to weeks in traditional kindling. Train duration and inter-train interval are key factors for rapid HK. Among the patterns, 12 trains/24h of 50Hz monophasic square wave with 10 s duration and 30 min interval between trains, is the best stimulus pattern for eliciting rapid dorsal HK.http://bcn.iums.ac.ir/browse.php?a_code=A-10-662-2&slc_lang=en&sid=1Dorsal hippocampus Electrical kindling Stimulation protocol |
| spellingShingle | Fataemeh Etemadi Mohammad Sayyah Hamid Gholami Pourbadi Vahab Babapour Assessment of the Optimal Stimulus Pattern to Achieve Rapid Dorsal Hippocampal Kindling in Rats Basic and Clinical Neuroscience Dorsal hippocampus Electrical kindling Stimulation protocol |
| title | Assessment of the Optimal Stimulus Pattern to Achieve Rapid Dorsal Hippocampal Kindling in Rats |
| title_full | Assessment of the Optimal Stimulus Pattern to Achieve Rapid Dorsal Hippocampal Kindling in Rats |
| title_fullStr | Assessment of the Optimal Stimulus Pattern to Achieve Rapid Dorsal Hippocampal Kindling in Rats |
| title_full_unstemmed | Assessment of the Optimal Stimulus Pattern to Achieve Rapid Dorsal Hippocampal Kindling in Rats |
| title_short | Assessment of the Optimal Stimulus Pattern to Achieve Rapid Dorsal Hippocampal Kindling in Rats |
| title_sort | assessment of the optimal stimulus pattern to achieve rapid dorsal hippocampal kindling in rats |
| topic | Dorsal hippocampus Electrical kindling Stimulation protocol |
| url | http://bcn.iums.ac.ir/browse.php?a_code=A-10-662-2&slc_lang=en&sid=1 |
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