The effect of hyperglycaemia on in vitro cytokine production and macrophage infection with Mycobacterium tuberculosis.
Type 2 diabetes mellitus is an established risk factor for tuberculosis but the underlying mechanisms are largely unknown. We examined the effects of hyperglycaemia, a hallmark of diabetes, on the cytokine response to and macrophage infection with Mycobacterium tuberculosis. Increasing in vitro gluc...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4322041?pdf=render |
| _version_ | 1818590405139103744 |
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| author | Ekta Lachmandas Frank Vrieling Louis G Wilson Simone A Joosten Mihai G Netea Tom H Ottenhoff Reinout van Crevel |
| author_facet | Ekta Lachmandas Frank Vrieling Louis G Wilson Simone A Joosten Mihai G Netea Tom H Ottenhoff Reinout van Crevel |
| author_sort | Ekta Lachmandas |
| collection | DOAJ |
| description | Type 2 diabetes mellitus is an established risk factor for tuberculosis but the underlying mechanisms are largely unknown. We examined the effects of hyperglycaemia, a hallmark of diabetes, on the cytokine response to and macrophage infection with Mycobacterium tuberculosis. Increasing in vitro glucose concentrations from 5 to 25 mmol/L had marginal effects on cytokine production following stimulation of peripheral blood mononuclear cells (PBMCs) with M. tuberculosis lysate, LPS or Candida albicans, while 40 mmol/L glucose increased production of TNF-α, IL-1β, IL-6 and IL-10, but not of IFN-γ, IL-17A and IL-22. Macrophage differentiation under hyperglycaemic conditions of 25 mmol/L glucose was also associated with increased cytokine production upon stimulation with M. tuberculosis lysate and LPS but in infection experiments no differences in M. tuberculosis killing or outgrowth was observed. The phagocytic capacity of these hyperglycaemic macrophages also remained unaltered. The fact that only very high glucose concentrations were able to significantly influence cytokine production by macrophages suggests that hyperglycaemia alone cannot fully explain the increased susceptibility of diabetes mellitus patients to tuberculosis. |
| first_indexed | 2024-12-16T09:56:01Z |
| format | Article |
| id | doaj.art-654bd959c93543248bf417ae1315fc7c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T09:56:01Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-654bd959c93543248bf417ae1315fc7c2022-12-21T22:35:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011794110.1371/journal.pone.0117941The effect of hyperglycaemia on in vitro cytokine production and macrophage infection with Mycobacterium tuberculosis.Ekta LachmandasFrank VrielingLouis G WilsonSimone A JoostenMihai G NeteaTom H OttenhoffReinout van CrevelType 2 diabetes mellitus is an established risk factor for tuberculosis but the underlying mechanisms are largely unknown. We examined the effects of hyperglycaemia, a hallmark of diabetes, on the cytokine response to and macrophage infection with Mycobacterium tuberculosis. Increasing in vitro glucose concentrations from 5 to 25 mmol/L had marginal effects on cytokine production following stimulation of peripheral blood mononuclear cells (PBMCs) with M. tuberculosis lysate, LPS or Candida albicans, while 40 mmol/L glucose increased production of TNF-α, IL-1β, IL-6 and IL-10, but not of IFN-γ, IL-17A and IL-22. Macrophage differentiation under hyperglycaemic conditions of 25 mmol/L glucose was also associated with increased cytokine production upon stimulation with M. tuberculosis lysate and LPS but in infection experiments no differences in M. tuberculosis killing or outgrowth was observed. The phagocytic capacity of these hyperglycaemic macrophages also remained unaltered. The fact that only very high glucose concentrations were able to significantly influence cytokine production by macrophages suggests that hyperglycaemia alone cannot fully explain the increased susceptibility of diabetes mellitus patients to tuberculosis.http://europepmc.org/articles/PMC4322041?pdf=render |
| spellingShingle | Ekta Lachmandas Frank Vrieling Louis G Wilson Simone A Joosten Mihai G Netea Tom H Ottenhoff Reinout van Crevel The effect of hyperglycaemia on in vitro cytokine production and macrophage infection with Mycobacterium tuberculosis. PLoS ONE |
| title | The effect of hyperglycaemia on in vitro cytokine production and macrophage infection with Mycobacterium tuberculosis. |
| title_full | The effect of hyperglycaemia on in vitro cytokine production and macrophage infection with Mycobacterium tuberculosis. |
| title_fullStr | The effect of hyperglycaemia on in vitro cytokine production and macrophage infection with Mycobacterium tuberculosis. |
| title_full_unstemmed | The effect of hyperglycaemia on in vitro cytokine production and macrophage infection with Mycobacterium tuberculosis. |
| title_short | The effect of hyperglycaemia on in vitro cytokine production and macrophage infection with Mycobacterium tuberculosis. |
| title_sort | effect of hyperglycaemia on in vitro cytokine production and macrophage infection with mycobacterium tuberculosis |
| url | http://europepmc.org/articles/PMC4322041?pdf=render |
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