Preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c
<strong><br /> Objective(s):</strong> The present work deals with the preparation of nanobioconjugates based on the immobilization of cytochrome c (cyt c) on functionalized multi-wall carbon nanotubes (f-MWCNTs). The effect of the nanosupport and the immobilization procedure on the...
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| Format: | Article |
| Language: | English |
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Iranian Society of Nanomedicine
2018-01-01
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| Series: | Nanomedicine Research Journal |
| Subjects: | |
| Online Access: | http://www.nanomedicine-rj.com/article_31895_25dd81ef368250092e3cad65264f27dd.pdf |
| _version_ | 1811279600585539584 |
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| author | Michaela Patila Evmorfia K. Diamanti Danai Bergouni Angeliki C. Polydera Dimitrios Gournis Haralambos Stamatis |
| author_facet | Michaela Patila Evmorfia K. Diamanti Danai Bergouni Angeliki C. Polydera Dimitrios Gournis Haralambos Stamatis |
| author_sort | Michaela Patila |
| collection | DOAJ |
| description | <strong><br /> Objective(s):</strong> The present work deals with the preparation of nanobioconjugates based on the immobilization of cytochrome c (cyt c) on functionalized multi-wall carbon nanotubes (f-MWCNTs). The effect of the nanosupport and the immobilization procedure on the biochemical and structural characteristics of the immobilized protein was investigated.
<strong>Methods: </strong>The MWCNTs were functionalized to provide alkyl chains with different length and terminal functional groups on their surface. The immobilization of cyt c was achieved through physical adsorption and covalent binding. Cyt-c-based nanoconjugates were characterized in terms of peroxidase activity and stability of protein, while UV-visible spectroscopy was used to investigate the structural characteristics of the immobilized protein.
<strong>Results:</strong> The loading of cyt c on f-MWCNTs was effectively achieved, with immobilization yields reaching up to 77%. The peroxidase activity of cyt c was higher in the case of non covalent immobilization compared to that of covalent procedure. Immobilized cyt c exhibited higher thermal stability than the native protein after 24 h incubation at 40<sup>o</sup>C, while it preserved up to 100% of its initial activity after incubation in the presence of a denaturing agent such as H<sub>2</sub>O<sub>2</sub>. No significant changes in the heme microenvironment of cyt c were observed in the presence of f-MWCNTs.
<strong>Conclusions:</strong> This study has demonstrated that f-MWCNTs are effective supports for the immobilization of cyt c, providing a universally applicable platform for the development of bionanoconjugates with potential use in a wide variety of fields in nanobiocatalysis, biosensing and nanomedicine. |
| first_indexed | 2024-04-13T00:57:27Z |
| format | Article |
| id | doaj.art-654cc0bbc1264d0997ff0798bcab1885 |
| institution | Directory Open Access Journal |
| issn | 2476-3489 2476-7123 |
| language | English |
| last_indexed | 2024-04-13T00:57:27Z |
| publishDate | 2018-01-01 |
| publisher | Iranian Society of Nanomedicine |
| record_format | Article |
| series | Nanomedicine Research Journal |
| spelling | doaj.art-654cc0bbc1264d0997ff0798bcab18852022-12-22T03:09:35ZengIranian Society of NanomedicineNanomedicine Research Journal2476-34892476-71232018-01-0131101810.22034/nmrj.2018.01.00231895Preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome cMichaela Patila0Evmorfia K. Diamanti1Danai Bergouni2Angeliki C. Polydera3Dimitrios Gournis4Haralambos Stamatis5Biotechnology Laboratory Department of Biological Applications and Technology, University of Ioannina,45110 Ioannina, GreeceDepartment of Materials Science and Engineering, University of Ioannina, 45110 Ioannina, GreeceDepartment of Materials Science and Engineering, University of Ioannina, 45110 Ioannina, GreeceBiotechnology Laboratory Department of Biological Applications and Technology, University of Ioannina, 45110 Ioannina, GreeceDepartment of Materials Science & Engineering, University of Ioannina, Ioannina, GreeceBiotechnology Laboratory Department of Biological Applications and Technology, University of Ioannina, 45110 Ioannina, Greece<strong><br /> Objective(s):</strong> The present work deals with the preparation of nanobioconjugates based on the immobilization of cytochrome c (cyt c) on functionalized multi-wall carbon nanotubes (f-MWCNTs). The effect of the nanosupport and the immobilization procedure on the biochemical and structural characteristics of the immobilized protein was investigated. <strong>Methods: </strong>The MWCNTs were functionalized to provide alkyl chains with different length and terminal functional groups on their surface. The immobilization of cyt c was achieved through physical adsorption and covalent binding. Cyt-c-based nanoconjugates were characterized in terms of peroxidase activity and stability of protein, while UV-visible spectroscopy was used to investigate the structural characteristics of the immobilized protein. <strong>Results:</strong> The loading of cyt c on f-MWCNTs was effectively achieved, with immobilization yields reaching up to 77%. The peroxidase activity of cyt c was higher in the case of non covalent immobilization compared to that of covalent procedure. Immobilized cyt c exhibited higher thermal stability than the native protein after 24 h incubation at 40<sup>o</sup>C, while it preserved up to 100% of its initial activity after incubation in the presence of a denaturing agent such as H<sub>2</sub>O<sub>2</sub>. No significant changes in the heme microenvironment of cyt c were observed in the presence of f-MWCNTs. <strong>Conclusions:</strong> This study has demonstrated that f-MWCNTs are effective supports for the immobilization of cyt c, providing a universally applicable platform for the development of bionanoconjugates with potential use in a wide variety of fields in nanobiocatalysis, biosensing and nanomedicine.http://www.nanomedicine-rj.com/article_31895_25dd81ef368250092e3cad65264f27dd.pdfcytochrome ccarbon nanotubesimmobilizationnanoconjugatebiocatalysis |
| spellingShingle | Michaela Patila Evmorfia K. Diamanti Danai Bergouni Angeliki C. Polydera Dimitrios Gournis Haralambos Stamatis Preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c Nanomedicine Research Journal cytochrome c carbon nanotubes immobilization nanoconjugate biocatalysis |
| title | Preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c |
| title_full | Preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c |
| title_fullStr | Preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c |
| title_full_unstemmed | Preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c |
| title_short | Preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c |
| title_sort | preparation and biochemical characterisation of nanoconjugates of functionalized carbon nanotubes and cytochrome c |
| topic | cytochrome c carbon nanotubes immobilization nanoconjugate biocatalysis |
| url | http://www.nanomedicine-rj.com/article_31895_25dd81ef368250092e3cad65264f27dd.pdf |
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