The Protective Role of <i>Bacillus velezensis</i> A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice
Zearalenone (ZEN) is an estrogen-like mycotoxin produced by <i>Fusarium</i> that seriously compromises the safety of animal and human health. In this study, our aim was to evaluate the protective effect of <i>Bacillus velezensis</i> A2 against biochemical and pathological cha...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-10-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6651/10/11/449 |
| _version_ | 1798005210110492672 |
|---|---|
| author | Nan Wang Peng Li Mingyang Wang Si Chen Sheng Huang Miao Long Shuhua Yang Jianbin He |
| author_facet | Nan Wang Peng Li Mingyang Wang Si Chen Sheng Huang Miao Long Shuhua Yang Jianbin He |
| author_sort | Nan Wang |
| collection | DOAJ |
| description | Zearalenone (ZEN) is an estrogen-like mycotoxin produced by <i>Fusarium</i> that seriously compromises the safety of animal and human health. In this study, our aim was to evaluate the protective effect of <i>Bacillus velezensis</i> A2 against biochemical and pathological changes induced by zearalenone in mice. Kunming mice (<i>n</i> = 40; 25 ± 2 g) were allotted to four treatment groups: a control group (basic feed); a ZEN group (basic feed with a ZEN dose of 60 mg/kg); an A2 strain fermented feed group (150 g of feed mixed with 150 mL of sterile distilled water and inoculated with 5 mL of phosphate buffer salt (PBS) resuspended A2 strain); and an A2 strain fermented ZEN-contaminated feed group. (A2 strain group 150 mL pure bacterial distilled water system mixed with 150 g ZEN-contaminated feed.) Our results showed that the <i>Bacillus velezensis</i> A2 strain can completely degrade the ZEN-contaminated feed within 5 days. (The concentration of ZEN in fermentation was 60 μg/mL.) After the mice fed for 28 days, compared with the control group, the activities of AST and ALT were increased, the activities of glutathione peroxidase (GSH-PX) and total superoxide dismutase (T-SOD) were decreased, and the amount of creatinine (CRE), blood urea nitrogen (BUN), uric acid (UA), and malondialdehyde (MDA) in the ZEN group were increased in the mice serum (<i>p</i> < 0.05; <i>p</i> < 0.01). However, compared with the ZEN group, these biochemical levels were reversed in the A2 strain fermented feed group and in the A2 strain fermented ZEN-contaminated feed group (<i>p</i> < 0.05; <i>p</i> < 0.01). Furthermore, histopathological analysis only showed pathological changes of the mice liver in the ZEN group. The results showed that <i>Bacillus velezensis</i> A2 as additive could effectively remove ZEN contamination in the feed and protect the mice against the toxic damage of ZEN. In conclusion, <i>Bacillus velezensis</i> A2 has great potential use as a microbial feed additive to detoxify the toxicity of zearalenone in production practice. |
| first_indexed | 2024-04-11T12:35:29Z |
| format | Article |
| id | doaj.art-654f91c059914172baa1fef12a7f980e |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T12:35:29Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-654f91c059914172baa1fef12a7f980e2022-12-22T04:23:37ZengMDPI AGToxins2072-66512018-10-01101144910.3390/toxins10110449toxins10110449The Protective Role of <i>Bacillus velezensis</i> A2 on the Biochemical and Hepatic Toxicity of Zearalenone in MiceNan Wang0Peng Li1Mingyang Wang2Si Chen3Sheng Huang4Miao Long5Shuhua Yang6Jianbin He7Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaKey Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, ChinaZearalenone (ZEN) is an estrogen-like mycotoxin produced by <i>Fusarium</i> that seriously compromises the safety of animal and human health. In this study, our aim was to evaluate the protective effect of <i>Bacillus velezensis</i> A2 against biochemical and pathological changes induced by zearalenone in mice. Kunming mice (<i>n</i> = 40; 25 ± 2 g) were allotted to four treatment groups: a control group (basic feed); a ZEN group (basic feed with a ZEN dose of 60 mg/kg); an A2 strain fermented feed group (150 g of feed mixed with 150 mL of sterile distilled water and inoculated with 5 mL of phosphate buffer salt (PBS) resuspended A2 strain); and an A2 strain fermented ZEN-contaminated feed group. (A2 strain group 150 mL pure bacterial distilled water system mixed with 150 g ZEN-contaminated feed.) Our results showed that the <i>Bacillus velezensis</i> A2 strain can completely degrade the ZEN-contaminated feed within 5 days. (The concentration of ZEN in fermentation was 60 μg/mL.) After the mice fed for 28 days, compared with the control group, the activities of AST and ALT were increased, the activities of glutathione peroxidase (GSH-PX) and total superoxide dismutase (T-SOD) were decreased, and the amount of creatinine (CRE), blood urea nitrogen (BUN), uric acid (UA), and malondialdehyde (MDA) in the ZEN group were increased in the mice serum (<i>p</i> < 0.05; <i>p</i> < 0.01). However, compared with the ZEN group, these biochemical levels were reversed in the A2 strain fermented feed group and in the A2 strain fermented ZEN-contaminated feed group (<i>p</i> < 0.05; <i>p</i> < 0.01). Furthermore, histopathological analysis only showed pathological changes of the mice liver in the ZEN group. The results showed that <i>Bacillus velezensis</i> A2 as additive could effectively remove ZEN contamination in the feed and protect the mice against the toxic damage of ZEN. In conclusion, <i>Bacillus velezensis</i> A2 has great potential use as a microbial feed additive to detoxify the toxicity of zearalenone in production practice.https://www.mdpi.com/2072-6651/10/11/449<i>Bacillus velezensis</i> A2zearalenone (ZEN)feed detoxificationserum enzymeoxidative damage |
| spellingShingle | Nan Wang Peng Li Mingyang Wang Si Chen Sheng Huang Miao Long Shuhua Yang Jianbin He The Protective Role of <i>Bacillus velezensis</i> A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice Toxins <i>Bacillus velezensis</i> A2 zearalenone (ZEN) feed detoxification serum enzyme oxidative damage |
| title | The Protective Role of <i>Bacillus velezensis</i> A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice |
| title_full | The Protective Role of <i>Bacillus velezensis</i> A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice |
| title_fullStr | The Protective Role of <i>Bacillus velezensis</i> A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice |
| title_full_unstemmed | The Protective Role of <i>Bacillus velezensis</i> A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice |
| title_short | The Protective Role of <i>Bacillus velezensis</i> A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice |
| title_sort | protective role of i bacillus velezensis i a2 on the biochemical and hepatic toxicity of zearalenone in mice |
| topic | <i>Bacillus velezensis</i> A2 zearalenone (ZEN) feed detoxification serum enzyme oxidative damage |
| url | https://www.mdpi.com/2072-6651/10/11/449 |
| work_keys_str_mv | AT nanwang theprotectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT pengli theprotectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT mingyangwang theprotectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT sichen theprotectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT shenghuang theprotectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT miaolong theprotectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT shuhuayang theprotectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT jianbinhe theprotectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT nanwang protectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT pengli protectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT mingyangwang protectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT sichen protectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT shenghuang protectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT miaolong protectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT shuhuayang protectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice AT jianbinhe protectiveroleofibacillusvelezensisia2onthebiochemicalandhepatictoxicityofzearalenoneinmice |