Enzyme characterization of lactic acid bacteria isolated from duck excreta
Background and Aim: The production of lignocellulosic biomass waste in the agricultural sector of Indonesia is quite high annually. Utilization of lignocellulosic biomass waste through fermentation technology can be used as feed and biofuel. Fermentation technology requires the involvement of micro-...
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| Format: | Article |
| Language: | English |
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Veterinary World
2024-01-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | https://www.veterinaryworld.org/Vol.17/January-2024/16.pdf |
| _version_ | 1797350601483354112 |
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| author | Dini Dwi Ludfiani Widya Asmara Forita Dyah Arianti |
| author_facet | Dini Dwi Ludfiani Widya Asmara Forita Dyah Arianti |
| author_sort | Dini Dwi Ludfiani |
| collection | DOAJ |
| description | Background and Aim: The production of lignocellulosic biomass waste in the agricultural sector of Indonesia is quite high annually. Utilization of lignocellulosic biomass waste through fermentation technology can be used as feed and biofuel. Fermentation technology requires the involvement of micro-organisms such as bacteria (lactic acid bacteria or LAB). LABs can be isolated from various sources, such as duck excreta. However, there have not been many reports of LAB from duck excreta. The present study aimed to characterize LAB enzymes isolated from duck excreta and obtain LAB enzymes with superior fermentation properties.
Materials and Methods: A total of 11 LAB cultures obtained from duck excreta in Yogyakarta, Indonesia, were tested. Enzyme characterization of each LAB was performed using the API ZYM kit (BioMérieux, Marcy-I’Etoile, France). The bacterial cell suspension was dropped onto the API ZYMTM cupule using a pipette and incubated for 4 h at 37°C. After incubation, ZYM A and ZYM B were dripped onto the API ZYM cupule, and color changes were observed for approximately 10 s under a strong light source.
Results: Esterase activity was moderate for all LABs. The activity of α-chymotrypsin, β-glucuronidase, α-fucosidase, and α-mannosidase was not observed in a total of 10 LAB. The phosphohydrolase and amino peptidase enzyme activity of seven LABs was strong. Only six LAB samples showed protease activity. The glycosyl hydrolase (GH) activity was observed in a total of 8 LAB, while the activity of 2 LAB was strong (Lactococcus lactis subsp. lactis K5 and Lactobacillus brevis M4A).
Conclusion: A total of 2 LABs have superior properties. L. lactis subsp. lactis K5 and L. brevis M4A have a high potential to be used in fermentation. They have the potential for further research, such as their effectiveness in fermentation, lignocellulose hydrolysis, feed additives, molecular characterization to detect specific enzymes, and their specific activities. |
| first_indexed | 2024-03-08T12:48:20Z |
| format | Article |
| id | doaj.art-6550eb4952b94f4ba9c4db0d0c396b48 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-03-08T12:48:20Z |
| publishDate | 2024-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-6550eb4952b94f4ba9c4db0d0c396b482024-01-21T03:49:34ZengVeterinary WorldVeterinary World0972-89882231-09162024-01-0117114314910.14202/vetworld.2024.143-149Enzyme characterization of lactic acid bacteria isolated from duck excretaDini Dwi Ludfiani0https://orcid.org/0000-0002-0773-3237Widya Asmara1https://orcid.org/0000-0001-6386-8892Forita Dyah Arianti2https://orcid.org/0000-0003-3789-7942Research Center for Sustainable Production Systems and Life Cycle Assessment, National Research and Innovation Agency (BRIN), Tangerang Selatan, Indonesia.Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.Research Center for Sustainable Production Systems and Life Cycle Assessment, National Research and Innovation Agency (BRIN), Tangerang Selatan, Indonesia.Background and Aim: The production of lignocellulosic biomass waste in the agricultural sector of Indonesia is quite high annually. Utilization of lignocellulosic biomass waste through fermentation technology can be used as feed and biofuel. Fermentation technology requires the involvement of micro-organisms such as bacteria (lactic acid bacteria or LAB). LABs can be isolated from various sources, such as duck excreta. However, there have not been many reports of LAB from duck excreta. The present study aimed to characterize LAB enzymes isolated from duck excreta and obtain LAB enzymes with superior fermentation properties. Materials and Methods: A total of 11 LAB cultures obtained from duck excreta in Yogyakarta, Indonesia, were tested. Enzyme characterization of each LAB was performed using the API ZYM kit (BioMérieux, Marcy-I’Etoile, France). The bacterial cell suspension was dropped onto the API ZYMTM cupule using a pipette and incubated for 4 h at 37°C. After incubation, ZYM A and ZYM B were dripped onto the API ZYM cupule, and color changes were observed for approximately 10 s under a strong light source. Results: Esterase activity was moderate for all LABs. The activity of α-chymotrypsin, β-glucuronidase, α-fucosidase, and α-mannosidase was not observed in a total of 10 LAB. The phosphohydrolase and amino peptidase enzyme activity of seven LABs was strong. Only six LAB samples showed protease activity. The glycosyl hydrolase (GH) activity was observed in a total of 8 LAB, while the activity of 2 LAB was strong (Lactococcus lactis subsp. lactis K5 and Lactobacillus brevis M4A). Conclusion: A total of 2 LABs have superior properties. L. lactis subsp. lactis K5 and L. brevis M4A have a high potential to be used in fermentation. They have the potential for further research, such as their effectiveness in fermentation, lignocellulose hydrolysis, feed additives, molecular characterization to detect specific enzymes, and their specific activities.https://www.veterinaryworld.org/Vol.17/January-2024/16.pdfapi zymduckenzymeexcretalactic acid bacteria |
| spellingShingle | Dini Dwi Ludfiani Widya Asmara Forita Dyah Arianti Enzyme characterization of lactic acid bacteria isolated from duck excreta Veterinary World api zym duck enzyme excreta lactic acid bacteria |
| title | Enzyme characterization of lactic acid bacteria isolated from duck excreta |
| title_full | Enzyme characterization of lactic acid bacteria isolated from duck excreta |
| title_fullStr | Enzyme characterization of lactic acid bacteria isolated from duck excreta |
| title_full_unstemmed | Enzyme characterization of lactic acid bacteria isolated from duck excreta |
| title_short | Enzyme characterization of lactic acid bacteria isolated from duck excreta |
| title_sort | enzyme characterization of lactic acid bacteria isolated from duck excreta |
| topic | api zym duck enzyme excreta lactic acid bacteria |
| url | https://www.veterinaryworld.org/Vol.17/January-2024/16.pdf |
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