The Effect of RBP4 on microRNA Expression Profiles in Porcine Granulosa Cells
Retinol binding protein 4 (RBP4) is a transporter of vitamin A that is secreted mainly by hepatocytes and adipocytes. It affects diverse pathophysiological processes, such as obesity, insulin resistance, and cardiovascular diseases. MicroRNAs (miRNAs) have been reported to play indispensable roles i...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/11/5/1391 |
| _version_ | 1797534237450043392 |
|---|---|
| author | Yun Zhao Jiahui Rao Tong Qiu Chunjin Li Xu Zhou |
| author_facet | Yun Zhao Jiahui Rao Tong Qiu Chunjin Li Xu Zhou |
| author_sort | Yun Zhao |
| collection | DOAJ |
| description | Retinol binding protein 4 (RBP4) is a transporter of vitamin A that is secreted mainly by hepatocytes and adipocytes. It affects diverse pathophysiological processes, such as obesity, insulin resistance, and cardiovascular diseases. MicroRNAs (miRNAs) have been reported to play indispensable roles in regulating various developmental processes via the post-transcriptional repression of target genes in mammals. However, the functional link between RBP4 and changes in miRNA expression in porcine granulosa cells (GCs) remains to be investigated. To examine how increased expression of <i>RBP4</i> affects miRNA expression, porcine GCs were infected with <i>RBP4</i>-targeted lentivirus for 72 h, and whole-genome miRNA profiling (miRNA sequencing) was performed. The sequencing data were validated using real-time quantitative polymerase chain reaction (RT-qPCR) analysis. As a result, we obtained 2783 known and 776 novel miRNAs. In the experimental group, 10 and seven miRNAs were significantly downregulated and upregulated, respectively, compared with the control group. Ontology analysis of the biological processes of these miRNAs indicated their involvement in a variety of biological functions. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that these miRNAs were involved mainly in the chemokine signaling pathway, peroxisome proliferators-activated receptors (PPAR) signaling pathway, insulin resistance pathway, nuclear factor-kappa B(NF-kappa B) signaling pathway, and steroid hormone biosynthesis. Our results indicate that RBP4 can regulate the expression of miRNAs in porcine GCs, with consequent physiological effects. In summary, this study profiling miRNA expression in <i>RBP4</i>-overexpressing porcine GCs provides an important reference point for future studies on the regulatory roles of miRNAs in the porcine reproductive system. |
| first_indexed | 2024-03-10T11:26:49Z |
| format | Article |
| id | doaj.art-6552ee94030747a1bdd36d912e2b3736 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T11:26:49Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-6552ee94030747a1bdd36d912e2b37362023-11-21T19:35:04ZengMDPI AGAnimals2076-26152021-05-01115139110.3390/ani11051391The Effect of RBP4 on microRNA Expression Profiles in Porcine Granulosa CellsYun Zhao0Jiahui Rao1Tong Qiu2Chunjin Li3Xu Zhou4College of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaRetinol binding protein 4 (RBP4) is a transporter of vitamin A that is secreted mainly by hepatocytes and adipocytes. It affects diverse pathophysiological processes, such as obesity, insulin resistance, and cardiovascular diseases. MicroRNAs (miRNAs) have been reported to play indispensable roles in regulating various developmental processes via the post-transcriptional repression of target genes in mammals. However, the functional link between RBP4 and changes in miRNA expression in porcine granulosa cells (GCs) remains to be investigated. To examine how increased expression of <i>RBP4</i> affects miRNA expression, porcine GCs were infected with <i>RBP4</i>-targeted lentivirus for 72 h, and whole-genome miRNA profiling (miRNA sequencing) was performed. The sequencing data were validated using real-time quantitative polymerase chain reaction (RT-qPCR) analysis. As a result, we obtained 2783 known and 776 novel miRNAs. In the experimental group, 10 and seven miRNAs were significantly downregulated and upregulated, respectively, compared with the control group. Ontology analysis of the biological processes of these miRNAs indicated their involvement in a variety of biological functions. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that these miRNAs were involved mainly in the chemokine signaling pathway, peroxisome proliferators-activated receptors (PPAR) signaling pathway, insulin resistance pathway, nuclear factor-kappa B(NF-kappa B) signaling pathway, and steroid hormone biosynthesis. Our results indicate that RBP4 can regulate the expression of miRNAs in porcine GCs, with consequent physiological effects. In summary, this study profiling miRNA expression in <i>RBP4</i>-overexpressing porcine GCs provides an important reference point for future studies on the regulatory roles of miRNAs in the porcine reproductive system.https://www.mdpi.com/2076-2615/11/5/1391granulosa cells (GCs)microRNAsretinol binding protein 4 (<i>RBP4</i>) |
| spellingShingle | Yun Zhao Jiahui Rao Tong Qiu Chunjin Li Xu Zhou The Effect of RBP4 on microRNA Expression Profiles in Porcine Granulosa Cells Animals granulosa cells (GCs) microRNAs retinol binding protein 4 (<i>RBP4</i>) |
| title | The Effect of RBP4 on microRNA Expression Profiles in Porcine Granulosa Cells |
| title_full | The Effect of RBP4 on microRNA Expression Profiles in Porcine Granulosa Cells |
| title_fullStr | The Effect of RBP4 on microRNA Expression Profiles in Porcine Granulosa Cells |
| title_full_unstemmed | The Effect of RBP4 on microRNA Expression Profiles in Porcine Granulosa Cells |
| title_short | The Effect of RBP4 on microRNA Expression Profiles in Porcine Granulosa Cells |
| title_sort | effect of rbp4 on microrna expression profiles in porcine granulosa cells |
| topic | granulosa cells (GCs) microRNAs retinol binding protein 4 (<i>RBP4</i>) |
| url | https://www.mdpi.com/2076-2615/11/5/1391 |
| work_keys_str_mv | AT yunzhao theeffectofrbp4onmicrornaexpressionprofilesinporcinegranulosacells AT jiahuirao theeffectofrbp4onmicrornaexpressionprofilesinporcinegranulosacells AT tongqiu theeffectofrbp4onmicrornaexpressionprofilesinporcinegranulosacells AT chunjinli theeffectofrbp4onmicrornaexpressionprofilesinporcinegranulosacells AT xuzhou theeffectofrbp4onmicrornaexpressionprofilesinporcinegranulosacells AT yunzhao effectofrbp4onmicrornaexpressionprofilesinporcinegranulosacells AT jiahuirao effectofrbp4onmicrornaexpressionprofilesinporcinegranulosacells AT tongqiu effectofrbp4onmicrornaexpressionprofilesinporcinegranulosacells AT chunjinli effectofrbp4onmicrornaexpressionprofilesinporcinegranulosacells AT xuzhou effectofrbp4onmicrornaexpressionprofilesinporcinegranulosacells |