Impact of mouse strain and sex when modeling radiation necrosis
Abstract Background Murine models are among the most common type of preclinical animal models used to study the human condition, but a wide selection of different mice is currently in use with these differences potentially compromising study results and impairing the ability to reconcile interstudy...
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Radiation Oncology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13014-020-01585-5 |
| _version_ | 1818388123189510144 |
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| author | Andrew J. Boria Carlos J. Perez-Torres |
| author_facet | Andrew J. Boria Carlos J. Perez-Torres |
| author_sort | Andrew J. Boria |
| collection | DOAJ |
| description | Abstract Background Murine models are among the most common type of preclinical animal models used to study the human condition, but a wide selection of different mice is currently in use with these differences potentially compromising study results and impairing the ability to reconcile interstudy results. Our goal was to determine how the strain and sex of the mice selection would affect the development of radiation necrosis in our murine model of radiation-induced cerebral necrosis. Methods We generated this model by using a preclinical irradiator to irradiate a sub-hemispheric portion of the brain of mice with single-fraction doses of 80 Gy. Eight possible combinations of mice made up of two different with two substrains each (BALB/cN, BALB/cJ, C57BL/6 N, and C57BL/6 J) and both sexes were irradiated in this study. Radiation necrosis development was tracked up to 8 weeks with a 7 T Bruker MRI utilizing T2-weighted and post-contrast T1-weighted imaging. MRI results were compared to and validated with the use of histology which utilized a scale from 0 to 3 in ascending order of damage. Results Both time post-irradiation and strain (BALB/c vs C57BL/6) were significant factors affecting radiation necrosis development. Sex was in general not a statistically significant parameter in terms of radiation necrosis development. Conclusion Mouse strain thus needs to be considered when evaluating the results of necrosis models. However, sex does not appear to be a variable needing major consideration. |
| first_indexed | 2024-12-14T04:20:50Z |
| format | Article |
| id | doaj.art-6554069a10a04607b9f8730c9caeb8ff |
| institution | Directory Open Access Journal |
| issn | 1748-717X |
| language | English |
| last_indexed | 2024-12-14T04:20:50Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Radiation Oncology |
| spelling | doaj.art-6554069a10a04607b9f8730c9caeb8ff2022-12-21T23:17:22ZengBMCRadiation Oncology1748-717X2020-06-011511710.1186/s13014-020-01585-5Impact of mouse strain and sex when modeling radiation necrosisAndrew J. Boria0Carlos J. Perez-Torres1School of Health Sciences, Purdue UniversitySchool of Health Sciences, Purdue UniversityAbstract Background Murine models are among the most common type of preclinical animal models used to study the human condition, but a wide selection of different mice is currently in use with these differences potentially compromising study results and impairing the ability to reconcile interstudy results. Our goal was to determine how the strain and sex of the mice selection would affect the development of radiation necrosis in our murine model of radiation-induced cerebral necrosis. Methods We generated this model by using a preclinical irradiator to irradiate a sub-hemispheric portion of the brain of mice with single-fraction doses of 80 Gy. Eight possible combinations of mice made up of two different with two substrains each (BALB/cN, BALB/cJ, C57BL/6 N, and C57BL/6 J) and both sexes were irradiated in this study. Radiation necrosis development was tracked up to 8 weeks with a 7 T Bruker MRI utilizing T2-weighted and post-contrast T1-weighted imaging. MRI results were compared to and validated with the use of histology which utilized a scale from 0 to 3 in ascending order of damage. Results Both time post-irradiation and strain (BALB/c vs C57BL/6) were significant factors affecting radiation necrosis development. Sex was in general not a statistically significant parameter in terms of radiation necrosis development. Conclusion Mouse strain thus needs to be considered when evaluating the results of necrosis models. However, sex does not appear to be a variable needing major consideration.http://link.springer.com/article/10.1186/s13014-020-01585-5Radiation necrosisSex as a biological variableMouse strainAnimal models |
| spellingShingle | Andrew J. Boria Carlos J. Perez-Torres Impact of mouse strain and sex when modeling radiation necrosis Radiation Oncology Radiation necrosis Sex as a biological variable Mouse strain Animal models |
| title | Impact of mouse strain and sex when modeling radiation necrosis |
| title_full | Impact of mouse strain and sex when modeling radiation necrosis |
| title_fullStr | Impact of mouse strain and sex when modeling radiation necrosis |
| title_full_unstemmed | Impact of mouse strain and sex when modeling radiation necrosis |
| title_short | Impact of mouse strain and sex when modeling radiation necrosis |
| title_sort | impact of mouse strain and sex when modeling radiation necrosis |
| topic | Radiation necrosis Sex as a biological variable Mouse strain Animal models |
| url | http://link.springer.com/article/10.1186/s13014-020-01585-5 |
| work_keys_str_mv | AT andrewjboria impactofmousestrainandsexwhenmodelingradiationnecrosis AT carlosjpereztorres impactofmousestrainandsexwhenmodelingradiationnecrosis |