Grain bran hydrolysates in the production of fruit distillates
Currently, there is an urgent need for domestic fermentation activators based on low-cost secondary raw materials. We aimed to study the effect of microbial enzyme preparations with different action on the hydrolysis of proteins and phytin of grain bran to obtain fermentation activators that could b...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Kemerovo State University
2023-03-01
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| Series: | Foods and Raw Materials |
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| Online Access: | https://jfrm.ru/en/issues/20672/20804/ |
| _version_ | 1827971452144451584 |
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| author | Ludmila N. Krikunova Elena P. Meleshkina Irina S. Vitol Elena V. Dubinina Olga N. Obodeeva |
| author_facet | Ludmila N. Krikunova Elena P. Meleshkina Irina S. Vitol Elena V. Dubinina Olga N. Obodeeva |
| author_sort | Ludmila N. Krikunova |
| collection | DOAJ |
| description | Currently, there is an urgent need for domestic fermentation activators based on low-cost secondary raw materials. We aimed to study the effect of microbial enzyme preparations with different action on the hydrolysis of proteins and phytin of grain bran to obtain fermentation activators that could become an alternative to imported ones.
We studied wheat and rye brans; microbial enzyme preparations with cytolytic, proteolytic, and phytase action; multi-enzyme compositions; and grain bran hydrolysates. Firstly, we determined the kinetic characteristics of enzyme preparations. Secondly, we evaluated their effectiveness in the hydrolysis of the brans. Thirdly, we developed multi-enzyme compositions. Finally, we determined the concentration of soluble forms of phosphorus and free amino acids in the hydrolysates.
We determined optimal temperature and pH values for the enzyme preparations. The multi-enzyme compositions contributed to a high accumulation of reducing substances, water-soluble protein, and phosphorus. The concentration of free amino acids in the hydrolysates obtained under the action of the bran’s own enzymes was about 20% higher in the wheat samples, compared to the rye samples. However, when using multi-enzyme compositions in addition to the bran’s own enzymes, the concentration of free amino acids was 1.5 times higher in the rye hydrolysates, compared to the wheat hydrolysates.
The use of multi-enzyme compositions under optimal conditions can double the content of phosphorus and free amino acids available for yeast, compared to the control. Our results can be used for further research into using grain bran hydrolysates as an alternative source of nitrogen and phosphorus nutrition for yeast at the fermentation stage of fruit distillate production. |
| first_indexed | 2024-04-09T19:09:11Z |
| format | Article |
| id | doaj.art-6557fd74d2fd457c961bc82edda38dfe |
| institution | Directory Open Access Journal |
| issn | 2308-4057 2310-9599 |
| language | English |
| last_indexed | 2024-04-09T19:09:11Z |
| publishDate | 2023-03-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Foods and Raw Materials |
| spelling | doaj.art-6557fd74d2fd457c961bc82edda38dfe2023-04-07T06:30:06ZengKemerovo State UniversityFoods and Raw Materials2308-40572310-95992023-03-01111354210.21603/2308-4057-2023-1-550Grain bran hydrolysates in the production of fruit distillatesLudmila N. Krikunova0https://orcid.org/0000-0002-7335-0453Elena P. Meleshkina1https://orcid.org/0000-0003-1339-7150Irina S. Vitol2https://orcid.org/0000-0001-5962-8909Elena V. Dubinina3https://orcid.org/0000-0002-8364-9539Olga N. Obodeeva4https://orcid.org/0000-0002-1068-4245All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry, Moscow, RussiaAll-Russian Scientific and Research Institute for Grain and Products of its Processing, Moscow, RussiaAll-Russian Scientific and Research Institute for Grain and Products of its Processing, Moscow, RussiaAll-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry, Moscow, RussiaAll-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry, Moscow, RussiaCurrently, there is an urgent need for domestic fermentation activators based on low-cost secondary raw materials. We aimed to study the effect of microbial enzyme preparations with different action on the hydrolysis of proteins and phytin of grain bran to obtain fermentation activators that could become an alternative to imported ones. We studied wheat and rye brans; microbial enzyme preparations with cytolytic, proteolytic, and phytase action; multi-enzyme compositions; and grain bran hydrolysates. Firstly, we determined the kinetic characteristics of enzyme preparations. Secondly, we evaluated their effectiveness in the hydrolysis of the brans. Thirdly, we developed multi-enzyme compositions. Finally, we determined the concentration of soluble forms of phosphorus and free amino acids in the hydrolysates. We determined optimal temperature and pH values for the enzyme preparations. The multi-enzyme compositions contributed to a high accumulation of reducing substances, water-soluble protein, and phosphorus. The concentration of free amino acids in the hydrolysates obtained under the action of the bran’s own enzymes was about 20% higher in the wheat samples, compared to the rye samples. However, when using multi-enzyme compositions in addition to the bran’s own enzymes, the concentration of free amino acids was 1.5 times higher in the rye hydrolysates, compared to the wheat hydrolysates. The use of multi-enzyme compositions under optimal conditions can double the content of phosphorus and free amino acids available for yeast, compared to the control. Our results can be used for further research into using grain bran hydrolysates as an alternative source of nitrogen and phosphorus nutrition for yeast at the fermentation stage of fruit distillate production.https://jfrm.ru/en/issues/20672/20804/grain branmicrobial enzyme preparationsmulti-enzyme compositionshydrolysatesfree amino acidssoluble forms of phosphorus |
| spellingShingle | Ludmila N. Krikunova Elena P. Meleshkina Irina S. Vitol Elena V. Dubinina Olga N. Obodeeva Grain bran hydrolysates in the production of fruit distillates Foods and Raw Materials grain bran microbial enzyme preparations multi-enzyme compositions hydrolysates free amino acids soluble forms of phosphorus |
| title | Grain bran hydrolysates in the production of fruit distillates |
| title_full | Grain bran hydrolysates in the production of fruit distillates |
| title_fullStr | Grain bran hydrolysates in the production of fruit distillates |
| title_full_unstemmed | Grain bran hydrolysates in the production of fruit distillates |
| title_short | Grain bran hydrolysates in the production of fruit distillates |
| title_sort | grain bran hydrolysates in the production of fruit distillates |
| topic | grain bran microbial enzyme preparations multi-enzyme compositions hydrolysates free amino acids soluble forms of phosphorus |
| url | https://jfrm.ru/en/issues/20672/20804/ |
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