RpoS regulates essential virulence factors remaining to be identified in Borrelia burgdorferi.
Since the RpoN-RpoS regulatory network was revealed in the Lyme disease spirochete Borrelia burgdorferi a decade ago, both upstream and downstream of the pathway have been intensively investigated. While significant progress has been made into understanding of how the network is regulated, most nota...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3531368?pdf=render |
| _version_ | 1828522384119824384 |
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| author | Qilong Xu Yanlin Shi Poonam Dadhwal Fang Ting Liang |
| author_facet | Qilong Xu Yanlin Shi Poonam Dadhwal Fang Ting Liang |
| author_sort | Qilong Xu |
| collection | DOAJ |
| description | Since the RpoN-RpoS regulatory network was revealed in the Lyme disease spirochete Borrelia burgdorferi a decade ago, both upstream and downstream of the pathway have been intensively investigated. While significant progress has been made into understanding of how the network is regulated, most notably, discovering a relationship of the network with Rrp2 and BosR, only three crucial virulence factors, including outer surface protein C (OspC) and decorin-binding proteins (Dbps) A and B, are associated with the pathway. Moreover, for more than 10 years no single RpoS-controlled gene has been found to be critical for infection, raising a question about whether additional RpoS-dependent virulence factors remain to be identified.The rpoS gene was deleted in B. burgdorferi; resulting mutants were modified to constitutively express all the known virulence factors, OspC, DbpA and DbpB. This genetic modification was unable to restore the rpoS mutant with infectivity.The inability to restore the rpoS mutant with infectivity by simultaneously over-expressing all the three virulence factors allows us to conclude RpoS also regulates essential genes that remain to be identified in B. burgdorferi. |
| first_indexed | 2024-12-11T20:06:54Z |
| format | Article |
| id | doaj.art-655ab872d54c495b84e493c9b0a12a56 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T20:06:54Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-655ab872d54c495b84e493c9b0a12a562022-12-22T00:52:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5321210.1371/journal.pone.0053212RpoS regulates essential virulence factors remaining to be identified in Borrelia burgdorferi.Qilong XuYanlin ShiPoonam DadhwalFang Ting LiangSince the RpoN-RpoS regulatory network was revealed in the Lyme disease spirochete Borrelia burgdorferi a decade ago, both upstream and downstream of the pathway have been intensively investigated. While significant progress has been made into understanding of how the network is regulated, most notably, discovering a relationship of the network with Rrp2 and BosR, only three crucial virulence factors, including outer surface protein C (OspC) and decorin-binding proteins (Dbps) A and B, are associated with the pathway. Moreover, for more than 10 years no single RpoS-controlled gene has been found to be critical for infection, raising a question about whether additional RpoS-dependent virulence factors remain to be identified.The rpoS gene was deleted in B. burgdorferi; resulting mutants were modified to constitutively express all the known virulence factors, OspC, DbpA and DbpB. This genetic modification was unable to restore the rpoS mutant with infectivity.The inability to restore the rpoS mutant with infectivity by simultaneously over-expressing all the three virulence factors allows us to conclude RpoS also regulates essential genes that remain to be identified in B. burgdorferi.http://europepmc.org/articles/PMC3531368?pdf=render |
| spellingShingle | Qilong Xu Yanlin Shi Poonam Dadhwal Fang Ting Liang RpoS regulates essential virulence factors remaining to be identified in Borrelia burgdorferi. PLoS ONE |
| title | RpoS regulates essential virulence factors remaining to be identified in Borrelia burgdorferi. |
| title_full | RpoS regulates essential virulence factors remaining to be identified in Borrelia burgdorferi. |
| title_fullStr | RpoS regulates essential virulence factors remaining to be identified in Borrelia burgdorferi. |
| title_full_unstemmed | RpoS regulates essential virulence factors remaining to be identified in Borrelia burgdorferi. |
| title_short | RpoS regulates essential virulence factors remaining to be identified in Borrelia burgdorferi. |
| title_sort | rpos regulates essential virulence factors remaining to be identified in borrelia burgdorferi |
| url | http://europepmc.org/articles/PMC3531368?pdf=render |
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