HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi <i>Marrubium vulgare</i> against Foodborne Diseases Bacteria
The present study aims to evaluate the chemical composition, metabolites secondary and pharmacology activities of methanolic extract of <i>Marrubium vulgare</i> collected from King Saudi Arabia. Moreover, the primary mode of action of the tested extract was studied here for the first tim...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/17/5112 |
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| author | Mayasar Al-Zaban Souheila Naghmouchi Nada K. AlHarbi |
| author_facet | Mayasar Al-Zaban Souheila Naghmouchi Nada K. AlHarbi |
| author_sort | Mayasar Al-Zaban |
| collection | DOAJ |
| description | The present study aims to evaluate the chemical composition, metabolites secondary and pharmacology activities of methanolic extract of <i>Marrubium vulgare</i> collected from King Saudi Arabia. Moreover, the primary mode of action of the tested extract was studied here for the first time against <i>E. coli</i> and <i>L. monocytogenes.</i> HPLC analysis shows that the major components in the tested extract are luteolin-7-<i>O</i>-<span style="font-variant: small-caps;">d</span>-glucoside, ferulic acid and premarrubiin. Obtained data demonstrated that the investigated extract was richer in phenol (26.8 ± 0.01 mg/GAE g) than in flavonoids (0.61 ± 0.05 mg EC/mL). In addition, the methanolic extract showed an important antioxidant capacity against the DPPH (IC<sub>50</sub> = 35 ± 0.01 µg/mL) and ABTS (IC<sub>50</sub> = 25 ± 0.2 µg/mL) radical scavenging and a strong inhibition of acetylcholinesterase enzyme with an IC<sub>50</sub> value corresponding to 0.4 mg/mL. The antibacterial activity demonstrated that the evaluated extract had significant activity against both Gram-positive and Gram-negative bacteria. The effect of time on cell integrity on <i>E. coli</i> and <i>L. monocytogenes</i> determined by time–kill and bacteriolysis tests showed that the <i>M. vulgare</i> extract reduced the viability of both strains after 8 and 10 h and had a bacteriolytic effect against two different categories of bacteria, Gram-positive and negative, which are not of the same potency. Based on obtained data, it can be concluded that Saudi <i>M. vulgare</i> has a high pharmacological importance and can be used in preparation of food or drugs. |
| first_indexed | 2024-03-10T08:06:50Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:06:50Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-655ada8331594e7699c7bfa32a91ab792023-11-22T10:59:14ZengMDPI AGMolecules1420-30492021-08-012617511210.3390/molecules26175112HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi <i>Marrubium vulgare</i> against Foodborne Diseases BacteriaMayasar Al-Zaban0Souheila Naghmouchi1Nada K. AlHarbi2Biology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi ArabiaBiology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi ArabiaBiology Department, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi ArabiaThe present study aims to evaluate the chemical composition, metabolites secondary and pharmacology activities of methanolic extract of <i>Marrubium vulgare</i> collected from King Saudi Arabia. Moreover, the primary mode of action of the tested extract was studied here for the first time against <i>E. coli</i> and <i>L. monocytogenes.</i> HPLC analysis shows that the major components in the tested extract are luteolin-7-<i>O</i>-<span style="font-variant: small-caps;">d</span>-glucoside, ferulic acid and premarrubiin. Obtained data demonstrated that the investigated extract was richer in phenol (26.8 ± 0.01 mg/GAE g) than in flavonoids (0.61 ± 0.05 mg EC/mL). In addition, the methanolic extract showed an important antioxidant capacity against the DPPH (IC<sub>50</sub> = 35 ± 0.01 µg/mL) and ABTS (IC<sub>50</sub> = 25 ± 0.2 µg/mL) radical scavenging and a strong inhibition of acetylcholinesterase enzyme with an IC<sub>50</sub> value corresponding to 0.4 mg/mL. The antibacterial activity demonstrated that the evaluated extract had significant activity against both Gram-positive and Gram-negative bacteria. The effect of time on cell integrity on <i>E. coli</i> and <i>L. monocytogenes</i> determined by time–kill and bacteriolysis tests showed that the <i>M. vulgare</i> extract reduced the viability of both strains after 8 and 10 h and had a bacteriolytic effect against two different categories of bacteria, Gram-positive and negative, which are not of the same potency. Based on obtained data, it can be concluded that Saudi <i>M. vulgare</i> has a high pharmacological importance and can be used in preparation of food or drugs.https://www.mdpi.com/1420-3049/26/17/5112antioxidant activityantibacterial activity<i>Marrubium vulgare</i>phenolic contentmethanolic extract |
| spellingShingle | Mayasar Al-Zaban Souheila Naghmouchi Nada K. AlHarbi HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi <i>Marrubium vulgare</i> against Foodborne Diseases Bacteria Molecules antioxidant activity antibacterial activity <i>Marrubium vulgare</i> phenolic content methanolic extract |
| title | HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi <i>Marrubium vulgare</i> against Foodborne Diseases Bacteria |
| title_full | HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi <i>Marrubium vulgare</i> against Foodborne Diseases Bacteria |
| title_fullStr | HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi <i>Marrubium vulgare</i> against Foodborne Diseases Bacteria |
| title_full_unstemmed | HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi <i>Marrubium vulgare</i> against Foodborne Diseases Bacteria |
| title_short | HPLC-Analysis, Biological Activities and Characterization of Action Mode of Saudi <i>Marrubium vulgare</i> against Foodborne Diseases Bacteria |
| title_sort | hplc analysis biological activities and characterization of action mode of saudi i marrubium vulgare i against foodborne diseases bacteria |
| topic | antioxidant activity antibacterial activity <i>Marrubium vulgare</i> phenolic content methanolic extract |
| url | https://www.mdpi.com/1420-3049/26/17/5112 |
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