Isolation and Characterization of Stigmasterol from Fritillaria roylei
Fritillaria roylei (Kshirakakoli) is the threatened species of “Ashtwarga” group suffers lot of confusion for identification & authentification in Ayurvedic system of medicine. Due to lack of natural sources and insufficient availability of kshirakakoli, chances of adulteration and substitution...
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| Format: | Article |
| Language: | English |
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State Islamic University Sunan Kalijaga
2020-11-01
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| Series: | Biology, Medicine & Natural Product Chemistry |
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| Online Access: | http://sciencebiology.org/index.php/BIOMEDICH/article/view/117 |
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| author | Gunpreet Kaur Vikas Gupta R G Singhal Parveen Bansal |
| author_facet | Gunpreet Kaur Vikas Gupta R G Singhal Parveen Bansal |
| author_sort | Gunpreet Kaur |
| collection | DOAJ |
| description | Fritillaria roylei (Kshirakakoli) is the threatened species of “Ashtwarga” group suffers lot of confusion for identification & authentification in Ayurvedic system of medicine. Due to lack of natural sources and insufficient availability of kshirakakoli, chances of adulteration and substitution increases which in turn leads to loss of faith of people in herbal drugs. Thus for identification and differentiation, quality standardization and quality assurance of kshirakakoli containing herbal formulations there is a need to isolate chemical marker compound using advanced analytical techniques. The methanol extract of root samples of plant was prepared and phytochemical screening was performed. Marker compound was isolated from the extract using column chromatography. Single compound having Rf value 0.31 was isolated with TLC by using mobile phase n-hexane: ethyl acetate: formic acid (8:2:0.1 v/v/v) and purified by re-crystallization with methanol. Isolated compound was further characterized by using melting point and spectral analysis. The methanol extract was dark brown in color and showed the presence of steroids, amino acids and flavonoids. The isolated compound was found to be white crystalline powder with melting point range of 167-169°C. Spectral analysis confirmed the presence of Stigmasterol. In present study stigmasterol was isolated for the first time and can be used as chemical marker for identification and differentiation of the plant from its substitutes. |
| first_indexed | 2024-12-16T12:51:08Z |
| format | Article |
| id | doaj.art-655d506e9f3844cba01f79b63a7ca10e |
| institution | Directory Open Access Journal |
| issn | 2089-6514 |
| language | English |
| last_indexed | 2024-12-16T12:51:08Z |
| publishDate | 2020-11-01 |
| publisher | State Islamic University Sunan Kalijaga |
| record_format | Article |
| series | Biology, Medicine & Natural Product Chemistry |
| spelling | doaj.art-655d506e9f3844cba01f79b63a7ca10e2022-12-21T22:31:08ZengState Islamic University Sunan KalijagaBiology, Medicine & Natural Product Chemistry2089-65142020-11-0192778010.14421/biomedich.2020.92.77-8091Isolation and Characterization of Stigmasterol from Fritillaria royleiGunpreet Kaur0Vikas Gupta1R G Singhal2Parveen Bansal3Baba Farid University of Health SciencesBaba Farid University of Health SciencesShobhit UniversityBaba Farid University of Health SciencesFritillaria roylei (Kshirakakoli) is the threatened species of “Ashtwarga” group suffers lot of confusion for identification & authentification in Ayurvedic system of medicine. Due to lack of natural sources and insufficient availability of kshirakakoli, chances of adulteration and substitution increases which in turn leads to loss of faith of people in herbal drugs. Thus for identification and differentiation, quality standardization and quality assurance of kshirakakoli containing herbal formulations there is a need to isolate chemical marker compound using advanced analytical techniques. The methanol extract of root samples of plant was prepared and phytochemical screening was performed. Marker compound was isolated from the extract using column chromatography. Single compound having Rf value 0.31 was isolated with TLC by using mobile phase n-hexane: ethyl acetate: formic acid (8:2:0.1 v/v/v) and purified by re-crystallization with methanol. Isolated compound was further characterized by using melting point and spectral analysis. The methanol extract was dark brown in color and showed the presence of steroids, amino acids and flavonoids. The isolated compound was found to be white crystalline powder with melting point range of 167-169°C. Spectral analysis confirmed the presence of Stigmasterol. In present study stigmasterol was isolated for the first time and can be used as chemical marker for identification and differentiation of the plant from its substitutes.http://sciencebiology.org/index.php/BIOMEDICH/article/view/117isolationkshirakakolimarkerstandardizationstigmasterol |
| spellingShingle | Gunpreet Kaur Vikas Gupta R G Singhal Parveen Bansal Isolation and Characterization of Stigmasterol from Fritillaria roylei Biology, Medicine & Natural Product Chemistry isolation kshirakakoli marker standardization stigmasterol |
| title | Isolation and Characterization of Stigmasterol from Fritillaria roylei |
| title_full | Isolation and Characterization of Stigmasterol from Fritillaria roylei |
| title_fullStr | Isolation and Characterization of Stigmasterol from Fritillaria roylei |
| title_full_unstemmed | Isolation and Characterization of Stigmasterol from Fritillaria roylei |
| title_short | Isolation and Characterization of Stigmasterol from Fritillaria roylei |
| title_sort | isolation and characterization of stigmasterol from fritillaria roylei |
| topic | isolation kshirakakoli marker standardization stigmasterol |
| url | http://sciencebiology.org/index.php/BIOMEDICH/article/view/117 |
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