Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60).
BACKGROUND: To prevent harmful autoimmunity most immune responses to self proteins are controlled by central and peripheral tolerance. T cells specific for a limited set of self-proteins such as human heat shock protein 60 (HSP60) may contribute to peripheral tolerance. It is not known whether HSP60...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3172234?pdf=render |
| _version_ | 1818061361263935488 |
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| author | Joost A Aalberse Berber Kapitein Sytze de Roock Mark R Klein Wilco de Jager Ruurd van der Zee Maarten O Hoekstra Femke van Wijk Berent J Prakken |
| author_facet | Joost A Aalberse Berber Kapitein Sytze de Roock Mark R Klein Wilco de Jager Ruurd van der Zee Maarten O Hoekstra Femke van Wijk Berent J Prakken |
| author_sort | Joost A Aalberse |
| collection | DOAJ |
| description | BACKGROUND: To prevent harmful autoimmunity most immune responses to self proteins are controlled by central and peripheral tolerance. T cells specific for a limited set of self-proteins such as human heat shock protein 60 (HSP60) may contribute to peripheral tolerance. It is not known whether HSP60-specific T cells are present at birth and thus may play a role in neonatal tolerance. We studied whether self-HSP60 reactive T cells are present in cord blood, and if so, what phenotype these cells have. METHODOLOGY/PRINCIPAL FINDINGS: Cord blood mononuclear cells (CBMC) of healthy, full term neonates (n = 21), were cultured with HSP60 and Tetanus Toxoid (TT) to study antigen specific proliferation, cytokine secretion and up-regulation of surface markers. The functional capacity of HSP60-induced T cells was determined with in vitro suppression assays. Stimulation of CBMC with HSP60 led to CD4(+) T cell proliferation and the production of various cytokines, most notably IL-10, Interferon-gamma, and IL-6. HSP60-induced T cells expressed FOXP3 and suppressed effector T cell responses in vitro. CONCLUSION: Self-reactive HSP60 specific T cells are already present at birth. Upon stimulation with self-HSP60 these cells proliferate, produce cytokines and express FOXP3. These cells function as suppressor cells in vitro and thus they may be involved in the regulation of neonatal immune responses. |
| first_indexed | 2024-12-10T13:47:05Z |
| format | Article |
| id | doaj.art-655d914378b049f98944337f9f9b636e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T13:47:05Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-655d914378b049f98944337f9f9b636e2022-12-22T01:46:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2411910.1371/journal.pone.0024119Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60).Joost A AalberseBerber KapiteinSytze de RoockMark R KleinWilco de JagerRuurd van der ZeeMaarten O HoekstraFemke van WijkBerent J PrakkenBACKGROUND: To prevent harmful autoimmunity most immune responses to self proteins are controlled by central and peripheral tolerance. T cells specific for a limited set of self-proteins such as human heat shock protein 60 (HSP60) may contribute to peripheral tolerance. It is not known whether HSP60-specific T cells are present at birth and thus may play a role in neonatal tolerance. We studied whether self-HSP60 reactive T cells are present in cord blood, and if so, what phenotype these cells have. METHODOLOGY/PRINCIPAL FINDINGS: Cord blood mononuclear cells (CBMC) of healthy, full term neonates (n = 21), were cultured with HSP60 and Tetanus Toxoid (TT) to study antigen specific proliferation, cytokine secretion and up-regulation of surface markers. The functional capacity of HSP60-induced T cells was determined with in vitro suppression assays. Stimulation of CBMC with HSP60 led to CD4(+) T cell proliferation and the production of various cytokines, most notably IL-10, Interferon-gamma, and IL-6. HSP60-induced T cells expressed FOXP3 and suppressed effector T cell responses in vitro. CONCLUSION: Self-reactive HSP60 specific T cells are already present at birth. Upon stimulation with self-HSP60 these cells proliferate, produce cytokines and express FOXP3. These cells function as suppressor cells in vitro and thus they may be involved in the regulation of neonatal immune responses.http://europepmc.org/articles/PMC3172234?pdf=render |
| spellingShingle | Joost A Aalberse Berber Kapitein Sytze de Roock Mark R Klein Wilco de Jager Ruurd van der Zee Maarten O Hoekstra Femke van Wijk Berent J Prakken Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60). PLoS ONE |
| title | Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60). |
| title_full | Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60). |
| title_fullStr | Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60). |
| title_full_unstemmed | Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60). |
| title_short | Cord blood CD4+ T cells respond to self heat shock protein 60 (HSP60). |
| title_sort | cord blood cd4 t cells respond to self heat shock protein 60 hsp60 |
| url | http://europepmc.org/articles/PMC3172234?pdf=render |
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