Subcutaneous Construction of Engineered Adipose Tissue with Fat Lobule-Like Structure Using Injectable Poly-Benzyl-L-Glutamate Microspheres Loaded with Adipose-Derived Stem Cells.
Porous microcarriers were fabricated from synthesized poly(γ-benzyl-L-glutamate) (PBLG) polymer to engineer adipose tissue with lobule-like structure via the injectable approach. The adipogenic differentiation of human adipose-derived stem cells (hASCs) seeded on porous PBLG microcarriers was determ...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4537260?pdf=render |
| _version_ | 1818161996308152320 |
|---|---|
| author | Wentao Sun Jianjun Fang Qi Yong Sufang Li Qingping Xie Jingbo Yin Lei Cui |
| author_facet | Wentao Sun Jianjun Fang Qi Yong Sufang Li Qingping Xie Jingbo Yin Lei Cui |
| author_sort | Wentao Sun |
| collection | DOAJ |
| description | Porous microcarriers were fabricated from synthesized poly(γ-benzyl-L-glutamate) (PBLG) polymer to engineer adipose tissue with lobule-like structure via the injectable approach. The adipogenic differentiation of human adipose-derived stem cells (hASCs) seeded on porous PBLG microcarriers was determined by adipogenic gene expression and glycerol-3-phosphate dehydrogenase enzyme activity. In vitro adipogenic cultivation was performed for 7 days, and induced hASC/PBLG complex (Adi-ASC/PBLG group) was subcutaneously injected into nude mice. Injections of PBLG microcarriers alone (PBLG group) and non-induced hASC/PBLG complex (ASC/PBLG group) served as controls. Newly formed tissues were harvested after 4 and 8 weeks. Generation of subcutaneous adipose tissue with typical lobule-like structure separated by fibrous septa was observed upon injection of adipogenic-induced hASC/microsphere complex. Adipogenesis significantly increased in the Adi-ASC/PBLG group compared with the control groups. The angiogenesis in the engineered adipose tissue was comparable to that in normal tissue as determined by capillary density and luminal diameter. Cell tracking assay demonstrated that labeled hASCs remained detectable in the neo-generated tissues 8 weeks post-injection using green fluorescence protein-labeled hASCs. These results indicate that adipose tissue with typical lobule-like structure could be engineered using injectable porous PBLG microspheres loaded with adipogenic-induced hASCs. |
| first_indexed | 2024-12-11T16:26:38Z |
| format | Article |
| id | doaj.art-655fdc7d4f3548418b238e282d889906 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T16:26:38Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-655fdc7d4f3548418b238e282d8899062022-12-22T00:58:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013561110.1371/journal.pone.0135611Subcutaneous Construction of Engineered Adipose Tissue with Fat Lobule-Like Structure Using Injectable Poly-Benzyl-L-Glutamate Microspheres Loaded with Adipose-Derived Stem Cells.Wentao SunJianjun FangQi YongSufang LiQingping XieJingbo YinLei CuiPorous microcarriers were fabricated from synthesized poly(γ-benzyl-L-glutamate) (PBLG) polymer to engineer adipose tissue with lobule-like structure via the injectable approach. The adipogenic differentiation of human adipose-derived stem cells (hASCs) seeded on porous PBLG microcarriers was determined by adipogenic gene expression and glycerol-3-phosphate dehydrogenase enzyme activity. In vitro adipogenic cultivation was performed for 7 days, and induced hASC/PBLG complex (Adi-ASC/PBLG group) was subcutaneously injected into nude mice. Injections of PBLG microcarriers alone (PBLG group) and non-induced hASC/PBLG complex (ASC/PBLG group) served as controls. Newly formed tissues were harvested after 4 and 8 weeks. Generation of subcutaneous adipose tissue with typical lobule-like structure separated by fibrous septa was observed upon injection of adipogenic-induced hASC/microsphere complex. Adipogenesis significantly increased in the Adi-ASC/PBLG group compared with the control groups. The angiogenesis in the engineered adipose tissue was comparable to that in normal tissue as determined by capillary density and luminal diameter. Cell tracking assay demonstrated that labeled hASCs remained detectable in the neo-generated tissues 8 weeks post-injection using green fluorescence protein-labeled hASCs. These results indicate that adipose tissue with typical lobule-like structure could be engineered using injectable porous PBLG microspheres loaded with adipogenic-induced hASCs.http://europepmc.org/articles/PMC4537260?pdf=render |
| spellingShingle | Wentao Sun Jianjun Fang Qi Yong Sufang Li Qingping Xie Jingbo Yin Lei Cui Subcutaneous Construction of Engineered Adipose Tissue with Fat Lobule-Like Structure Using Injectable Poly-Benzyl-L-Glutamate Microspheres Loaded with Adipose-Derived Stem Cells. PLoS ONE |
| title | Subcutaneous Construction of Engineered Adipose Tissue with Fat Lobule-Like Structure Using Injectable Poly-Benzyl-L-Glutamate Microspheres Loaded with Adipose-Derived Stem Cells. |
| title_full | Subcutaneous Construction of Engineered Adipose Tissue with Fat Lobule-Like Structure Using Injectable Poly-Benzyl-L-Glutamate Microspheres Loaded with Adipose-Derived Stem Cells. |
| title_fullStr | Subcutaneous Construction of Engineered Adipose Tissue with Fat Lobule-Like Structure Using Injectable Poly-Benzyl-L-Glutamate Microspheres Loaded with Adipose-Derived Stem Cells. |
| title_full_unstemmed | Subcutaneous Construction of Engineered Adipose Tissue with Fat Lobule-Like Structure Using Injectable Poly-Benzyl-L-Glutamate Microspheres Loaded with Adipose-Derived Stem Cells. |
| title_short | Subcutaneous Construction of Engineered Adipose Tissue with Fat Lobule-Like Structure Using Injectable Poly-Benzyl-L-Glutamate Microspheres Loaded with Adipose-Derived Stem Cells. |
| title_sort | subcutaneous construction of engineered adipose tissue with fat lobule like structure using injectable poly benzyl l glutamate microspheres loaded with adipose derived stem cells |
| url | http://europepmc.org/articles/PMC4537260?pdf=render |
| work_keys_str_mv | AT wentaosun subcutaneousconstructionofengineeredadiposetissuewithfatlobulelikestructureusinginjectablepolybenzyllglutamatemicrospheresloadedwithadiposederivedstemcells AT jianjunfang subcutaneousconstructionofengineeredadiposetissuewithfatlobulelikestructureusinginjectablepolybenzyllglutamatemicrospheresloadedwithadiposederivedstemcells AT qiyong subcutaneousconstructionofengineeredadiposetissuewithfatlobulelikestructureusinginjectablepolybenzyllglutamatemicrospheresloadedwithadiposederivedstemcells AT sufangli subcutaneousconstructionofengineeredadiposetissuewithfatlobulelikestructureusinginjectablepolybenzyllglutamatemicrospheresloadedwithadiposederivedstemcells AT qingpingxie subcutaneousconstructionofengineeredadiposetissuewithfatlobulelikestructureusinginjectablepolybenzyllglutamatemicrospheresloadedwithadiposederivedstemcells AT jingboyin subcutaneousconstructionofengineeredadiposetissuewithfatlobulelikestructureusinginjectablepolybenzyllglutamatemicrospheresloadedwithadiposederivedstemcells AT leicui subcutaneousconstructionofengineeredadiposetissuewithfatlobulelikestructureusinginjectablepolybenzyllglutamatemicrospheresloadedwithadiposederivedstemcells |