Expression of Staphylococcus aureus Virulence Factors in Atopic Dermatitis
Atopic dermatitis (AD) is a skin inflammatory disease in which the opportunistic pathogen Staphylococcus aureus is prevalent and abundant. S. aureus harbors several secreted virulence factors that have well-studied functions in infection models, but it is unclear whether these extracellular microbia...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-07-01
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| Series: | JID Innovations |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667026722000388 |
| _version_ | 1811221531744796672 |
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| author | Si En Poh Winston L.C. Koh Shi Yu Derek Lim Etienne C.E. Wang Yik Weng Yew John E.A. Common Hazel H. Oon Hao Li |
| author_facet | Si En Poh Winston L.C. Koh Shi Yu Derek Lim Etienne C.E. Wang Yik Weng Yew John E.A. Common Hazel H. Oon Hao Li |
| author_sort | Si En Poh |
| collection | DOAJ |
| description | Atopic dermatitis (AD) is a skin inflammatory disease in which the opportunistic pathogen Staphylococcus aureus is prevalent and abundant. S. aureus harbors several secreted virulence factors that have well-studied functions in infection models, but it is unclear whether these extracellular microbial factors are relevant in the context of AD. To address this question, we designed a culture-independent method to detect and quantify S. aureus virulence factors expressed at the skin sites. We utilized RNase-H‒dependent multiplex PCR for preamplification of reverse-transcribed RNA extracted from tape strips of patients with AD sampled at skin sites with differing severity and assessed the expression of a panel of S. aureus virulence factors using qPCR. We observed an increase in viable S. aureus abundance on sites with increased severity of disease, and many virulence factors were expressed at the AD skin sites. Surprisingly, we did not observe any significant upregulation of the virulence factors at the lesional sites compared with those at the nonlesional control. Overall, we utilized a robust assay to directly detect and quantify viable S. aureus and its associated virulence factors at the site of AD skin lesions. This method can be extended to study the expression of skin microbial genes at the sites of various dermatological conditions. |
| first_indexed | 2024-04-12T08:00:58Z |
| format | Article |
| id | doaj.art-656009753971462ea223eaf419383db4 |
| institution | Directory Open Access Journal |
| issn | 2667-0267 |
| language | English |
| last_indexed | 2024-04-12T08:00:58Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JID Innovations |
| spelling | doaj.art-656009753971462ea223eaf419383db42022-12-22T03:41:19ZengElsevierJID Innovations2667-02672022-07-0124100130Expression of Staphylococcus aureus Virulence Factors in Atopic DermatitisSi En Poh0Winston L.C. Koh1Shi Yu Derek Lim2Etienne C.E. Wang3Yik Weng Yew4John E.A. Common5Hazel H. Oon6Hao Li7Molecular Engineering Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research, Singapore, SingaporeMolecular Engineering Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research, Singapore, SingaporeNational Skin Centre, National Healthcare Group, Singapore, SingaporeNational Skin Centre, National Healthcare Group, Singapore, SingaporeNational Skin Centre, National Healthcare Group, Singapore, SingaporeA∗STAR Skin Research Labs (A∗SRL), Agency for Science, Technology and Research, Singapore, SingaporeNational Skin Centre, National Healthcare Group, Singapore, SingaporeMolecular Engineering Lab, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research, Singapore, Singapore; Department of Chemistry, Faculty of Science, National University of Singapore, Singapore, Singapore; Correspondence: Hao Li, Department of Chemistry, Faculty of Science, National University of Singapore, 4 Science Drive 2, S9 Level 12, Singapore 117544, Singapore.Atopic dermatitis (AD) is a skin inflammatory disease in which the opportunistic pathogen Staphylococcus aureus is prevalent and abundant. S. aureus harbors several secreted virulence factors that have well-studied functions in infection models, but it is unclear whether these extracellular microbial factors are relevant in the context of AD. To address this question, we designed a culture-independent method to detect and quantify S. aureus virulence factors expressed at the skin sites. We utilized RNase-H‒dependent multiplex PCR for preamplification of reverse-transcribed RNA extracted from tape strips of patients with AD sampled at skin sites with differing severity and assessed the expression of a panel of S. aureus virulence factors using qPCR. We observed an increase in viable S. aureus abundance on sites with increased severity of disease, and many virulence factors were expressed at the AD skin sites. Surprisingly, we did not observe any significant upregulation of the virulence factors at the lesional sites compared with those at the nonlesional control. Overall, we utilized a robust assay to directly detect and quantify viable S. aureus and its associated virulence factors at the site of AD skin lesions. This method can be extended to study the expression of skin microbial genes at the sites of various dermatological conditions.http://www.sciencedirect.com/science/article/pii/S2667026722000388 |
| spellingShingle | Si En Poh Winston L.C. Koh Shi Yu Derek Lim Etienne C.E. Wang Yik Weng Yew John E.A. Common Hazel H. Oon Hao Li Expression of Staphylococcus aureus Virulence Factors in Atopic Dermatitis JID Innovations |
| title | Expression of Staphylococcus aureus Virulence Factors in Atopic Dermatitis |
| title_full | Expression of Staphylococcus aureus Virulence Factors in Atopic Dermatitis |
| title_fullStr | Expression of Staphylococcus aureus Virulence Factors in Atopic Dermatitis |
| title_full_unstemmed | Expression of Staphylococcus aureus Virulence Factors in Atopic Dermatitis |
| title_short | Expression of Staphylococcus aureus Virulence Factors in Atopic Dermatitis |
| title_sort | expression of staphylococcus aureus virulence factors in atopic dermatitis |
| url | http://www.sciencedirect.com/science/article/pii/S2667026722000388 |
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