The effects of CoenzymeQ10 on gentamicin induced nephrotoxicity
Abstract Background: Gentamicin (GM) is one the aminoglycoside antibiotics which isroutinelyused to treatinfections gram-negative, either alone or insynergistic withbeta-lactamantibioticsused. However, frequent useleads toserious side effectssuch asrenal toxicity, ototoxicity. Coenzyme Q10 has anti...
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Arak Medical University
2016-10-01
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| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
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| Online Access: | http://amuj.arakmu.ac.ir/article-1-4250-en.pdf |
| _version_ | 1819175334845612032 |
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| author | Zeinab Hameidi Zad Saeed Hajihashemi Ali Rahbari Fatemeh Ghanbari |
| author_facet | Zeinab Hameidi Zad Saeed Hajihashemi Ali Rahbari Fatemeh Ghanbari |
| author_sort | Zeinab Hameidi Zad |
| collection | DOAJ |
| description | Abstract
Background: Gentamicin (GM) is one the aminoglycoside antibiotics which isroutinelyused to treatinfections gram-negative, either alone or insynergistic withbeta-lactamantibioticsused. However, frequent useleads toserious side effectssuch asrenal toxicity, ototoxicity. Coenzyme Q10 has antioxidant, anti-inflammatory and vasodilatory properties. According to these properties of Coenzyme Q10 and tissue damage mechanism in GM induced-nephrotoxicity, in this study, the effects of these two substances for the co-treatment and post -treatment on renal injury induced by gentamicin were investigated.
Materials and Methods: Experiments has been done on 77 male Wistar rats in weight range of 200 to 250 g. Animals were divided randomly into 5 groups of 7 numbers. Renal nephrotoxicity induced by i.p injection of gentamicin (100mg/kg) Therapeutic effect of coenzyme Q10 (10mg/kg)in the two protocols co-treatment and post-treatmentwas investigated.The animals after the last injectionon the ninth day of co-treatment andthe seventeenth day of post-treatmentwere placed into individual metabolic cages so as to collection urine and urine volume was measured gravimetrically. Afteranesthesia, systolic blood pressure and renal blood flow was measured. Then blood sampling was done. Amount of urea, creatinin, sodium, potassium and osmolarity was measured in plasma and urine samples. Left kidney, for doing histological experiments in 10% buffered formaldehyde and right kidney for biochemical experiments in fluid nitrogen was preserved.
Results: Co-treatment with Coenzyme Q10 significantly decreased fractional excretion of sodium (6.37±1.33 %; p<0.001) and decreased fractional excretion of potassium(219.14±83.8 %; p<0.001) MDA levels (2.13 ±0.24µmol/gkw; p<0.001), and significantly increased renal blood flow (6.38 ±0.1ml/min: p<0.01) and FRAP levels (24.44±0.42mmol/gkw; p<0.001). Post-treatment with coenzyme Q10 significantly decreased fractional excretion of sodium (3.58 ±0.57 %; p<0.001), potassium (111.77±29.4%; p<0.001) and MDA levels (3.08 ±0.12µmol/gkw; p<0.001) and significantly increased renal blood flow (6.74±0.15ml/min: p<0.001) and FRAP levels (24.34±0.75mmol/gkw; p<0.001) that is reduced by gentamicin.
Conclusion: According to the results, this study showed thatpost- treatment with coenzyme Q10more protective effect on the kidney tissue andAnda greater increase inantioxidant defensecreated. |
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| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-12-22T20:53:13Z |
| publishDate | 2016-10-01 |
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| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-65603b8eca0b464f929f7f9da340cb6c2022-12-21T18:13:01ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2016-10-011972235The effects of CoenzymeQ10 on gentamicin induced nephrotoxicityZeinab Hameidi Zad0Saeed Hajihashemi1Ali Rahbari2Fatemeh Ghanbari3MSc student of Physiology, Department of Physiology, School of Medicine, Arak University of Medical Sciences.Assistant Professor of Physiology, Department of Physiology, School of Medicine, Arak University of Medical Sciences.Assistant Professor of Pathology, Department of Pathology , Arak University of Medical Sciences.Assistant Professor of Pharmacology, Department of Pharmacology, Islamic Azad University of Arak. Abstract Background: Gentamicin (GM) is one the aminoglycoside antibiotics which isroutinelyused to treatinfections gram-negative, either alone or insynergistic withbeta-lactamantibioticsused. However, frequent useleads toserious side effectssuch asrenal toxicity, ototoxicity. Coenzyme Q10 has antioxidant, anti-inflammatory and vasodilatory properties. According to these properties of Coenzyme Q10 and tissue damage mechanism in GM induced-nephrotoxicity, in this study, the effects of these two substances for the co-treatment and post -treatment on renal injury induced by gentamicin were investigated. Materials and Methods: Experiments has been done on 77 male Wistar rats in weight range of 200 to 250 g. Animals were divided randomly into 5 groups of 7 numbers. Renal nephrotoxicity induced by i.p injection of gentamicin (100mg/kg) Therapeutic effect of coenzyme Q10 (10mg/kg)in the two protocols co-treatment and post-treatmentwas investigated.The animals after the last injectionon the ninth day of co-treatment andthe seventeenth day of post-treatmentwere placed into individual metabolic cages so as to collection urine and urine volume was measured gravimetrically. Afteranesthesia, systolic blood pressure and renal blood flow was measured. Then blood sampling was done. Amount of urea, creatinin, sodium, potassium and osmolarity was measured in plasma and urine samples. Left kidney, for doing histological experiments in 10% buffered formaldehyde and right kidney for biochemical experiments in fluid nitrogen was preserved. Results: Co-treatment with Coenzyme Q10 significantly decreased fractional excretion of sodium (6.37±1.33 %; p<0.001) and decreased fractional excretion of potassium(219.14±83.8 %; p<0.001) MDA levels (2.13 ±0.24µmol/gkw; p<0.001), and significantly increased renal blood flow (6.38 ±0.1ml/min: p<0.01) and FRAP levels (24.44±0.42mmol/gkw; p<0.001). Post-treatment with coenzyme Q10 significantly decreased fractional excretion of sodium (3.58 ±0.57 %; p<0.001), potassium (111.77±29.4%; p<0.001) and MDA levels (3.08 ±0.12µmol/gkw; p<0.001) and significantly increased renal blood flow (6.74±0.15ml/min: p<0.001) and FRAP levels (24.34±0.75mmol/gkw; p<0.001) that is reduced by gentamicin. Conclusion: According to the results, this study showed thatpost- treatment with coenzyme Q10more protective effect on the kidney tissue andAnda greater increase inantioxidant defensecreated.http://amuj.arakmu.ac.ir/article-1-4250-en.pdfNephrotoxicityGentamicincoenzyme Q10cobalamin |
| spellingShingle | Zeinab Hameidi Zad Saeed Hajihashemi Ali Rahbari Fatemeh Ghanbari The effects of CoenzymeQ10 on gentamicin induced nephrotoxicity Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk Nephrotoxicity Gentamicin coenzyme Q10 cobalamin |
| title | The effects of CoenzymeQ10 on gentamicin induced nephrotoxicity |
| title_full | The effects of CoenzymeQ10 on gentamicin induced nephrotoxicity |
| title_fullStr | The effects of CoenzymeQ10 on gentamicin induced nephrotoxicity |
| title_full_unstemmed | The effects of CoenzymeQ10 on gentamicin induced nephrotoxicity |
| title_short | The effects of CoenzymeQ10 on gentamicin induced nephrotoxicity |
| title_sort | effects of coenzymeq10 on gentamicin induced nephrotoxicity |
| topic | Nephrotoxicity Gentamicin coenzyme Q10 cobalamin |
| url | http://amuj.arakmu.ac.ir/article-1-4250-en.pdf |
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