Alteration of circulating unconventional T cells in cerebral ischemia: an observational study
Abstract Immune reactions provoked by cerebral ischemia play crucial roles in the pathogenesis of brain damage and contribute to tissue regeneration processes. While functions of many immune cell types in post-ischemic inflammation have been well studied in experimental stroke, the exact roles playe...
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| Format: | Article |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14343-2 |
| _version_ | 1811242217576071168 |
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| author | Chao Zhou Wei Rao Xinhua Zhou Dan He Zhen Li Nyambayar Dashtsoodol Yue Ren |
| author_facet | Chao Zhou Wei Rao Xinhua Zhou Dan He Zhen Li Nyambayar Dashtsoodol Yue Ren |
| author_sort | Chao Zhou |
| collection | DOAJ |
| description | Abstract Immune reactions provoked by cerebral ischemia play crucial roles in the pathogenesis of brain damage and contribute to tissue regeneration processes. While functions of many immune cell types in post-ischemic inflammation have been well studied in experimental stroke, the exact roles played by unconventional T cells in pathogenesis of the clinical stroke remain to be precisely determined. In the present study, we investigated the frequencies and absolute cell numbers of peripheral blood T lymphocyte subpopulations including those of invariant natural killer T (iNKT) cells, CD3+CD56+ NKT-like (NKTL) cells, and γδ T cells from patients with acute cerebral infarction (ACI), chronic cerebrovascular disease (CCD) or chronic cerebral circulation insufficiency (CCI) by flow cytometry, and analyzed their association with the disease severity and the clinical outcome. We observed significantly reduced cell numbers of circulating iNKT cells, NKTL cells and γδ T cells in cerebral ischemia patients as compared with the healthy controls. Of note, we also demonstrated that numbers of peripheral blood iNKT and γδ T cells are significantly reduced in patients with ACI when compared among different cerebral ischemia patient groups. Moreover, the reduced number of iNKT cells is significantly associated with the disease severity and recovery in cerebral ischemia patients. Our results demonstrate for the first time the reduction of peripheral blood NKTL, iNKT and γδ T cells in patients with the cerebral ischemia, and particularly reduced iNKT and γδ T cells in the acute phase. The reduction of iNKT cells seems to be significantly associated with the disease severity and recovery. We hope that our findings might lead to the identification of predictive and prognostic values of human peripheral unconventional T cell subsets in the cerebral ischemia. |
| first_indexed | 2024-04-12T13:48:30Z |
| format | Article |
| id | doaj.art-6560ec33bc8c45cab885320bd05aa532 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T13:48:30Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6560ec33bc8c45cab885320bd05aa5322022-12-22T03:30:37ZengNature PortfolioScientific Reports2045-23222022-06-011211810.1038/s41598-022-14343-2Alteration of circulating unconventional T cells in cerebral ischemia: an observational studyChao Zhou0Wei Rao1Xinhua Zhou2Dan He3Zhen Li4Nyambayar Dashtsoodol5Yue Ren6Department of Neurology, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeDepartment of Neurology, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeDepartment of Neurology, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeThe Neurological Institute of Jiangxi Province, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeThe Neurological Institute of Jiangxi Province, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeDepartment of Immunology and Stem Cell Biology, Faculty of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa UniversityThe Neurological Institute of Jiangxi Province, Jiangxi Provincial People’s Hospital, The First Affiliated Hospital of Nanchang Medical CollegeAbstract Immune reactions provoked by cerebral ischemia play crucial roles in the pathogenesis of brain damage and contribute to tissue regeneration processes. While functions of many immune cell types in post-ischemic inflammation have been well studied in experimental stroke, the exact roles played by unconventional T cells in pathogenesis of the clinical stroke remain to be precisely determined. In the present study, we investigated the frequencies and absolute cell numbers of peripheral blood T lymphocyte subpopulations including those of invariant natural killer T (iNKT) cells, CD3+CD56+ NKT-like (NKTL) cells, and γδ T cells from patients with acute cerebral infarction (ACI), chronic cerebrovascular disease (CCD) or chronic cerebral circulation insufficiency (CCI) by flow cytometry, and analyzed their association with the disease severity and the clinical outcome. We observed significantly reduced cell numbers of circulating iNKT cells, NKTL cells and γδ T cells in cerebral ischemia patients as compared with the healthy controls. Of note, we also demonstrated that numbers of peripheral blood iNKT and γδ T cells are significantly reduced in patients with ACI when compared among different cerebral ischemia patient groups. Moreover, the reduced number of iNKT cells is significantly associated with the disease severity and recovery in cerebral ischemia patients. Our results demonstrate for the first time the reduction of peripheral blood NKTL, iNKT and γδ T cells in patients with the cerebral ischemia, and particularly reduced iNKT and γδ T cells in the acute phase. The reduction of iNKT cells seems to be significantly associated with the disease severity and recovery. We hope that our findings might lead to the identification of predictive and prognostic values of human peripheral unconventional T cell subsets in the cerebral ischemia.https://doi.org/10.1038/s41598-022-14343-2 |
| spellingShingle | Chao Zhou Wei Rao Xinhua Zhou Dan He Zhen Li Nyambayar Dashtsoodol Yue Ren Alteration of circulating unconventional T cells in cerebral ischemia: an observational study Scientific Reports |
| title | Alteration of circulating unconventional T cells in cerebral ischemia: an observational study |
| title_full | Alteration of circulating unconventional T cells in cerebral ischemia: an observational study |
| title_fullStr | Alteration of circulating unconventional T cells in cerebral ischemia: an observational study |
| title_full_unstemmed | Alteration of circulating unconventional T cells in cerebral ischemia: an observational study |
| title_short | Alteration of circulating unconventional T cells in cerebral ischemia: an observational study |
| title_sort | alteration of circulating unconventional t cells in cerebral ischemia an observational study |
| url | https://doi.org/10.1038/s41598-022-14343-2 |
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