A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis
Abstract Background Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a central component for extracellular protein production, there has been a lac...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-04-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-017-0688-7 |
| _version_ | 1819206111922749440 |
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| author | Dongbo Cai Hao Wang Penghui He Chengjun Zhu Qin Wang Xuetuan Wei Christopher T. Nomura Shouwen Chen |
| author_facet | Dongbo Cai Hao Wang Penghui He Chengjun Zhu Qin Wang Xuetuan Wei Christopher T. Nomura Shouwen Chen |
| author_sort | Dongbo Cai |
| collection | DOAJ |
| description | Abstract Background Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a central component for extracellular protein production, there has been a lack of research on whether protein secretion could be enhanced via overexpression of signal peptide peptidases. Results In this study, both nattokinase and α-amylase were employed as prototypical secreted target proteins to evaluate the function of putative signal peptide peptidases (SppA and TepA) in Bacillus licheniformis. We observed dramatic decreases in the concentrations of both target proteins (45 and 49%, respectively) in a sppA deficient strain, while the extracellular protein yields of nattokinase and α-amylase were increased by 30 and 67% respectively in a strain overexpressing SppA. In addition, biomass, specific enzyme activities and the relative gene transcriptional levels were also enhanced due to the overexpression of sppA, while altering the expression levels of tepA had no effect on the concentrations of the secreted target proteins. Conclusions Our results confirm that SppA, but not TepA, plays an important functional role for protein secretion in B. licheniformis. Our results indicate that the sppA overexpression strain, B. licheniformis BL10GS, could be used as a promising host strain for the industrial production of heterologous secreted proteins. |
| first_indexed | 2024-12-23T05:02:25Z |
| format | Article |
| id | doaj.art-656123c6096a4ef2b4319932e7b4b7c4 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-23T05:02:25Z |
| publishDate | 2017-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-656123c6096a4ef2b4319932e7b4b7c42022-12-21T17:59:11ZengBMCMicrobial Cell Factories1475-28592017-04-0116111010.1186/s12934-017-0688-7A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformisDongbo Cai0Hao Wang1Penghui He2Chengjun Zhu3Qin Wang4Xuetuan Wei5Christopher T. Nomura6Shouwen Chen7Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei UniversityCollege of Food Science and Technology, Huazhong Agricultural UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei UniversityAbstract Background Signal peptide peptidases play an important role in the removal of remnant signal peptides in the cell membrane, a critical step for extracellular protein production. Although these proteins are likely a central component for extracellular protein production, there has been a lack of research on whether protein secretion could be enhanced via overexpression of signal peptide peptidases. Results In this study, both nattokinase and α-amylase were employed as prototypical secreted target proteins to evaluate the function of putative signal peptide peptidases (SppA and TepA) in Bacillus licheniformis. We observed dramatic decreases in the concentrations of both target proteins (45 and 49%, respectively) in a sppA deficient strain, while the extracellular protein yields of nattokinase and α-amylase were increased by 30 and 67% respectively in a strain overexpressing SppA. In addition, biomass, specific enzyme activities and the relative gene transcriptional levels were also enhanced due to the overexpression of sppA, while altering the expression levels of tepA had no effect on the concentrations of the secreted target proteins. Conclusions Our results confirm that SppA, but not TepA, plays an important functional role for protein secretion in B. licheniformis. Our results indicate that the sppA overexpression strain, B. licheniformis BL10GS, could be used as a promising host strain for the industrial production of heterologous secreted proteins.http://link.springer.com/article/10.1186/s12934-017-0688-7Bacillus licheniformisSignal peptide peptidaseProtein secretionsppAtepA |
| spellingShingle | Dongbo Cai Hao Wang Penghui He Chengjun Zhu Qin Wang Xuetuan Wei Christopher T. Nomura Shouwen Chen A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis Microbial Cell Factories Bacillus licheniformis Signal peptide peptidase Protein secretion sppA tepA |
| title | A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis |
| title_full | A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis |
| title_fullStr | A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis |
| title_full_unstemmed | A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis |
| title_short | A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis |
| title_sort | novel strategy to improve protein secretion via overexpression of the sppa signal peptide peptidase in bacillus licheniformis |
| topic | Bacillus licheniformis Signal peptide peptidase Protein secretion sppA tepA |
| url | http://link.springer.com/article/10.1186/s12934-017-0688-7 |
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