Discriminating between Terminal- and Non-Terminal Respiratory Unit-Type Lung Adenocarcinoma Based on MicroRNA Profiles.
Lung adenocarcinomas can be classified into terminal respiratory unit (TRU) and non-TRU types. We previously reported that non-TRU-type adenocarcinoma has unique clinical and morphological features as compared to the TRU type. Here we investigated whether micro (mi)RNA expression profiles can be use...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5004921?pdf=render |
| _version_ | 1818421887185715200 |
|---|---|
| author | Mi-Hyun Kim Jeong Su Cho Yeongdae Kim Chang Hun Lee Min Ki Lee Dong Hoon Shin |
| author_facet | Mi-Hyun Kim Jeong Su Cho Yeongdae Kim Chang Hun Lee Min Ki Lee Dong Hoon Shin |
| author_sort | Mi-Hyun Kim |
| collection | DOAJ |
| description | Lung adenocarcinomas can be classified into terminal respiratory unit (TRU) and non-TRU types. We previously reported that non-TRU-type adenocarcinoma has unique clinical and morphological features as compared to the TRU type. Here we investigated whether micro (mi)RNA expression profiles can be used to distinguish between these two subtypes of lung adenocarcinoma. The expression of 1205 human and 144 human viral miRNAs was analyzed in TRU- and non-TRU-type lung adenocarcinoma samples (n = 4 each) by microarray. Results were validated by quantitative real-time (qRT-)PCR and in situ hybridization. A comparison of miRNA profiles revealed 29 miRNAs that were differentially expressed between TRU- and non-TRU adenocarcinoma types. Specifically, hsa-miR-494 and ebv-miR-BART19 were up regulated by > 5-fold, whereas hsa-miR-551b was down regulated by > 5-fold in the non-TRU relative to the TRU type. The miRNA signature was confirmed by qRT-PCR analysis using an independent set of paired adenocarcinoma (non-TRU-type, n = 21 and TRU-type, n = 12) and normal tissue samples. Non-TRU samples showed increased expression of miR-494 (p = 0.033) and ebv-miR-BART19 (p = 0.001) as compared to TRU-type samples. Both miRNAs were weakly expressed in the TRU type but strongly expressed in the non-TRU type. Neither subtype showed miR-551b expression. TRU- and non-TRU-type adenocarcinomas have distinct miRNA expression profiles, suggesting that tumorigenesis in lung adenocarcinoma occur via different pathways. |
| first_indexed | 2024-12-14T13:17:30Z |
| format | Article |
| id | doaj.art-65633dd41a384ea5811f9944a200cf13 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T13:17:30Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-65633dd41a384ea5811f9944a200cf132022-12-21T23:00:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01118e016099610.1371/journal.pone.0160996Discriminating between Terminal- and Non-Terminal Respiratory Unit-Type Lung Adenocarcinoma Based on MicroRNA Profiles.Mi-Hyun KimJeong Su ChoYeongdae KimChang Hun LeeMin Ki LeeDong Hoon ShinLung adenocarcinomas can be classified into terminal respiratory unit (TRU) and non-TRU types. We previously reported that non-TRU-type adenocarcinoma has unique clinical and morphological features as compared to the TRU type. Here we investigated whether micro (mi)RNA expression profiles can be used to distinguish between these two subtypes of lung adenocarcinoma. The expression of 1205 human and 144 human viral miRNAs was analyzed in TRU- and non-TRU-type lung adenocarcinoma samples (n = 4 each) by microarray. Results were validated by quantitative real-time (qRT-)PCR and in situ hybridization. A comparison of miRNA profiles revealed 29 miRNAs that were differentially expressed between TRU- and non-TRU adenocarcinoma types. Specifically, hsa-miR-494 and ebv-miR-BART19 were up regulated by > 5-fold, whereas hsa-miR-551b was down regulated by > 5-fold in the non-TRU relative to the TRU type. The miRNA signature was confirmed by qRT-PCR analysis using an independent set of paired adenocarcinoma (non-TRU-type, n = 21 and TRU-type, n = 12) and normal tissue samples. Non-TRU samples showed increased expression of miR-494 (p = 0.033) and ebv-miR-BART19 (p = 0.001) as compared to TRU-type samples. Both miRNAs were weakly expressed in the TRU type but strongly expressed in the non-TRU type. Neither subtype showed miR-551b expression. TRU- and non-TRU-type adenocarcinomas have distinct miRNA expression profiles, suggesting that tumorigenesis in lung adenocarcinoma occur via different pathways.http://europepmc.org/articles/PMC5004921?pdf=render |
| spellingShingle | Mi-Hyun Kim Jeong Su Cho Yeongdae Kim Chang Hun Lee Min Ki Lee Dong Hoon Shin Discriminating between Terminal- and Non-Terminal Respiratory Unit-Type Lung Adenocarcinoma Based on MicroRNA Profiles. PLoS ONE |
| title | Discriminating between Terminal- and Non-Terminal Respiratory Unit-Type Lung Adenocarcinoma Based on MicroRNA Profiles. |
| title_full | Discriminating between Terminal- and Non-Terminal Respiratory Unit-Type Lung Adenocarcinoma Based on MicroRNA Profiles. |
| title_fullStr | Discriminating between Terminal- and Non-Terminal Respiratory Unit-Type Lung Adenocarcinoma Based on MicroRNA Profiles. |
| title_full_unstemmed | Discriminating between Terminal- and Non-Terminal Respiratory Unit-Type Lung Adenocarcinoma Based on MicroRNA Profiles. |
| title_short | Discriminating between Terminal- and Non-Terminal Respiratory Unit-Type Lung Adenocarcinoma Based on MicroRNA Profiles. |
| title_sort | discriminating between terminal and non terminal respiratory unit type lung adenocarcinoma based on microrna profiles |
| url | http://europepmc.org/articles/PMC5004921?pdf=render |
| work_keys_str_mv | AT mihyunkim discriminatingbetweenterminalandnonterminalrespiratoryunittypelungadenocarcinomabasedonmicrornaprofiles AT jeongsucho discriminatingbetweenterminalandnonterminalrespiratoryunittypelungadenocarcinomabasedonmicrornaprofiles AT yeongdaekim discriminatingbetweenterminalandnonterminalrespiratoryunittypelungadenocarcinomabasedonmicrornaprofiles AT changhunlee discriminatingbetweenterminalandnonterminalrespiratoryunittypelungadenocarcinomabasedonmicrornaprofiles AT minkilee discriminatingbetweenterminalandnonterminalrespiratoryunittypelungadenocarcinomabasedonmicrornaprofiles AT donghoonshin discriminatingbetweenterminalandnonterminalrespiratoryunittypelungadenocarcinomabasedonmicrornaprofiles |