Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering
Inspired by the aligned extracellular matrix and bioceramics in human bone tissue, we investigated the relative contributions of nanotopography and equine bone powders (EBPs) with human dental pulp stem cells (DPSCs) to the osteogenesis. Both nanotopography and EBPs independently promoted the osteog...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/7/2398 |
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| author | Kyoung-Je Jang Sujin Kim Sangbae Park Woochan Kim Yonghyun Gwon Sunho Park Ki-Taek Lim Hoon Seonwoo Jangho Kim |
| author_facet | Kyoung-Je Jang Sujin Kim Sangbae Park Woochan Kim Yonghyun Gwon Sunho Park Ki-Taek Lim Hoon Seonwoo Jangho Kim |
| author_sort | Kyoung-Je Jang |
| collection | DOAJ |
| description | Inspired by the aligned extracellular matrix and bioceramics in human bone tissue, we investigated the relative contributions of nanotopography and equine bone powders (EBPs) with human dental pulp stem cells (DPSCs) to the osteogenesis. Both nanotopography and EBPs independently promoted the osteogenesis of DPSCs, osteogenesis was further promoted by the two factors in combination, indicating the importance of synergistic design factor of guided bone regeneration (GBR) membrane. The osteogenesis of DPSCs was affected by the polycaprolactone-based nanotopography of parallel nanogrooves as well as EBPs coating. Interestingly, both nanopattern and EBPs affected the DPSCs morphologies; nanopattern led to cell elongation and EBPs led to cell spreading and clustering. Analysis of the DPSCs-substrate interaction, DPSCs-EBPs interaction suggests that the combined environment of both factors play a crucial role in mediating osteogenic phenotype. This simple method to achieve a suitable environment for osteogenesis via nanotopography and EBPs coating modulation may be regarded as a promising technique for GBR/GTR membranes, which widely used dental and maxillofacial surgery applications. |
| first_indexed | 2024-03-10T20:45:55Z |
| format | Article |
| id | doaj.art-656410db521049cdba7e7c905561936a |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T20:45:55Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-656410db521049cdba7e7c905561936a2023-11-19T20:21:01ZengMDPI AGApplied Sciences2076-34172020-04-01107239810.3390/app10072398Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue EngineeringKyoung-Je Jang0Sujin Kim1Sangbae Park2Woochan Kim3Yonghyun Gwon4Sunho Park5Ki-Taek Lim6Hoon Seonwoo7Jangho Kim8Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, KoreaDepartment of Rural and Biosystems Engineering, Chonnam National University, Gwangju 61186, KoreaDepartment of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, KoreaDepartment of Rural and Biosystems Engineering, Chonnam National University, Gwangju 61186, KoreaDepartment of Rural and Biosystems Engineering, Chonnam National University, Gwangju 61186, KoreaDepartment of Rural and Biosystems Engineering, Chonnam National University, Gwangju 61186, KoreaDepartment of Biosystems Engineering, College of Agricultural and Life Sciences, Kangwon National University, Chuncheon 24341, KoreaDepartment of Industrial Mechanical Engineering, College of Life Science and Natural Resources, Sunchon National University, Sunchon 57922, KoreaDepartment of Rural and Biosystems Engineering, Chonnam National University, Gwangju 61186, KoreaInspired by the aligned extracellular matrix and bioceramics in human bone tissue, we investigated the relative contributions of nanotopography and equine bone powders (EBPs) with human dental pulp stem cells (DPSCs) to the osteogenesis. Both nanotopography and EBPs independently promoted the osteogenesis of DPSCs, osteogenesis was further promoted by the two factors in combination, indicating the importance of synergistic design factor of guided bone regeneration (GBR) membrane. The osteogenesis of DPSCs was affected by the polycaprolactone-based nanotopography of parallel nanogrooves as well as EBPs coating. Interestingly, both nanopattern and EBPs affected the DPSCs morphologies; nanopattern led to cell elongation and EBPs led to cell spreading and clustering. Analysis of the DPSCs-substrate interaction, DPSCs-EBPs interaction suggests that the combined environment of both factors play a crucial role in mediating osteogenic phenotype. This simple method to achieve a suitable environment for osteogenesis via nanotopography and EBPs coating modulation may be regarded as a promising technique for GBR/GTR membranes, which widely used dental and maxillofacial surgery applications.https://www.mdpi.com/2076-3417/10/7/2398nanogroovesPCL patchesequine bone powderdental pulp stem cells |
| spellingShingle | Kyoung-Je Jang Sujin Kim Sangbae Park Woochan Kim Yonghyun Gwon Sunho Park Ki-Taek Lim Hoon Seonwoo Jangho Kim Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering Applied Sciences nanogrooves PCL patches equine bone powder dental pulp stem cells |
| title | Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering |
| title_full | Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering |
| title_fullStr | Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering |
| title_full_unstemmed | Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering |
| title_short | Lithographically-Fabricated HA-Incorporated PCL Nanopatterned Patch for Tissue Engineering |
| title_sort | lithographically fabricated ha incorporated pcl nanopatterned patch for tissue engineering |
| topic | nanogrooves PCL patches equine bone powder dental pulp stem cells |
| url | https://www.mdpi.com/2076-3417/10/7/2398 |
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